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167,150 results

Showing: 241 - 270 of 167150 results

  1. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  2. pET28-GG-PUF

    Plasmid
    #52137
    Purpose
    Receiving vector for PUM-HD assembly and expression in E.coli
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Type
    Empty backbone
    Tags
    PUM-HD flanking region 1' and PUM-HD flankin…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    52137-plasmid-map-sequence-id-80978

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  3. pJD849

    Plasmid
    #245490
    Purpose
    Binary Plasmid for plant transformation expressing RUBY from the 35S promoter and the terminator from the Arabidopsis thaliana HSP gene. Kanamycin plant selection
    Depositor
    Beth Rowan
    Insert
    p35S-RUBY
    Expression
    Plant
    Promoter
    35S
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245490-plasmid-map-sequence-id-488119

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  7. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Available Since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

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  9. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available Since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  11. TFORF1106

    Plasmid
    #143762
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EPAS1 (EPAS1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143762-plasmid-map-sequence-id-385793

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  22. mKeima-Red-Mito-7

    Plasmid
    #56018
    Purpose
    Localization: Mitochondria, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    mKeima-Red
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    56018-plasmid-map-sequence-id-201345

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  27. pGL3 Basic Vector

    Plasmid
    #212936
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available Since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212936-plasmid-map-sequence-id-419196

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  28. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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Showing: 241 - 270 of 167150 results