173,903 results

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EF1a-TBET-T2A-mCHERRY

Plasmid

#224296

Purpose

Expresses mouse TBET and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

TBET T2A mCherry (Tbx21 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHflu1

Plasmid

#203878

Purpose

pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer

Depositor

Bogumil Karas

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc Metap2

Plasmid

#199685

Purpose

Transient expression of Metap2

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJK345

Plasmid

#71559

Purpose

Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

alanine racemase

Tags

His6

Expression

Bacterial

Mutation

deletes alanine-367 and alanine-368

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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EBFP2-H2B-6

Plasmid

#55243

Purpose

Localization: Nucleus/Histones, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

EBFP2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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HyPer7.2DAAO-NLS

Plasmid

#168302

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-bARGSer-AxeTxe

Plasmid

#192473

Purpose

Second-generation bacterial acoustic reporter gene derived from Serratia

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Inserts

bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Native promoter from Enterococcus faecium and pBAD

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-TERT

Plasmid

#51631

Purpose

Mammalian expression of 3xFLAG tagged TERT, N-terminal.

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TERT (TERT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFOK

Plasmid

#166651

Purpose

Suicide vector for gene manipulation

Depositor

Dirk Bumann

Article

Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

Insert

I-sceI, sacB

Use

Synthetic Biology

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-RSET-iGABASnFR2-6xHis

Plasmid

#218885

Purpose

Bacterial expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Tags

6xHis

Expression

Bacterial

Mutation

S99A F102Y F104Y L178S

Promoter

T7

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal TDP43 GFP

Plasmid

#27467

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Tags

GFP

Expression

Yeast

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pTU2-AmyE-Pveg-eGFP

Plasmid

#112792

Purpose

Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

Pveg-eGFP

Expression

Bacterial

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEV53D

Plasmid

#44168

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Equine infectious anemia virus (EIAV) gag-pol,rev, and S2

Use

Lentiviral

Expression

Mammalian

Mutation

A131T mutation in gag; Deletion of viral LTR sequ…

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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ADORA1-DuET

Plasmid

#213173

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADORA1 (ADORA1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg_opt-3xFlag

Plasmid

#223251

Purpose

Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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HA-SFPQ

Plasmid

#166959

Purpose

Expresses HA-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJRD215

Plasmid

#167555

Purpose

Backbone plasmid to pRMA154

Depositor

Renato Morona

Article

Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

Type

Empty backbone

Use

Broad host range mobilisable

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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mEos3.2-N1

Plasmid

#54525

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson, Tao Xu

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-hAXL

Plasmid

#124797

Purpose

Tet-inducible AXL expression in mammalian cells

Depositor

Kenneth Pienta

Article

Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

Insert

AXL (AXL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-TurboID-NES_pLX304

Plasmid

#202019

Purpose

expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-TurboID-NES

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

Plasmid

#138418

Purpose

CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Promoter

EF1a

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSVG

Plasmid

#11912

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, A206K relative the wild type GFP sequ…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
amilCP chromoprotein

Plasmid

#117847

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Article

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_c-MYC

Plasmid

#26818

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-c-MYC-3'UTR (MYC Human)

Expression

Mammalian

Mutation

Sequence begins from second ATG; Synthetic UTR se…

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

Plasmid

#20942

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

EF1alpha

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST53-LRRK2-WT

Plasmid

#25044

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)

Plasmid

#242593

Purpose

A Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin, mCherry-zCdt1

Use

Zebrafish expressio

Promoter

zebrafish Ubi

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pOSIP-KL-mcherry

Plasmid

#116947

Purpose

Integrating mCherry into lambda attB in E. coli chromosome.

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

mcherry

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV_3XHA-ATP6V1B2-mNeonGreen

Plasmid

#228865

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA and mNeonGreen

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p6XHis_NLS-SaCas9

Plasmid

#101086

Purpose

Express SaCas9 in bacteria with a 6xHis tag for purification

Depositor

Rick Tarleton

Article

Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

Insert

CRISPR-associated protein Cas9/Csn1

Use

CRISPR

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS307

Plasmid

#51785

Purpose

an integrating (YIp) vector with a LYS2 selectable marker

Depositor

David Stillman

Article

Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

Type

Empty backbone

Expression

Yeast

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLM494

Plasmid

#100539

Purpose

CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

Inserts

GGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase

Use

Cre/Lox and Synthetic Biology

Expression

Yeast

Promoter

PGK1, TDH3, TIP1, and ZEO1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-ChRmine-mScarlet-WPRE

Plasmid

#130994

Purpose

AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIND21-puro_SOX10-OLIG2-NKX6.2-3xFLAG

Plasmid

#250320

Purpose

Tetracycline-inducible expression of SOX10-OLIG2-NKX6.2 as polyprotein linked by self cleavage sites P2A and T2A. rtTA (Tet-ON) is driven the EF1alpha promoter.

Depositor

Michael Wehr

Article

Raabe et al Cells. 2022 Jan 11;11(2):241. doi: 10.33

Insert

SOX10-P2A-OLIG2-T2A-NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE2

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-KRAS4B(G12V)

Plasmid

#35633

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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