4,073 results

1. pRc/RSV-m CBP-HA

   Plasmid

   #16701

   Depositor

   Richard Goodman

   Article

   Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBMN YFP-LID

   Plasmid

   #31767

   Depositor

   Thomas Wandless

   Article

   Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

   Insert

   YFP-LID (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

   Promoter

   LTR

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_Luciferase

   Plasmid

   #25894

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   Luciferase
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tetO-ALN

   Plasmid

   #43918

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Gylcine-serine-glycine (GSG) linker followed by v…

   Promoter

   TRE promoter, Tet-ON

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQLinkN

   Plasmid

   #13670

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FU-tet-o-hOct4

   Plasmid

   #19778

   Depositor

   Konrad Hochedlinger

   Article

   Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

   Insert

   Oct4 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 Flag FKHR AAA mutant

   Plasmid

   #13508

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   FKHR AAA (FOXO1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Promoter

   Available since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBabe-puro Ras V12

   Plasmid

   #1768

   Depositor

   Bob Weinberg

   Insert

   ras V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V, constitutively active

   Promoter

   Available since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWZL Neo Myr Flag TBK1

    Plasmid

    #20648

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSin-EF2-Nanog-Pur

    Plasmid

    #16578

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens Nanog homeobox (NANOG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p1324 HPV-16 E7

    Plasmid

    #8643

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E7 (E7 Human papillomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

13. mRuby2-C1

    Plasmid

    #54768

    Purpose

    Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAMPK alpha2 K45R

    Plasmid

    #15992

    Depositor

    Morris Birnbaum

    Article

    Mu et al Mol Cell. 2001 May . 7(5):1085-94.

    Insert

    AMPK alpha2 (Prkaa2 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K45R

    Promoter

    CMV

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CaMKIIa-eNpHR 3.0-EYFP

    Plasmid

    #26971

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Has Service

    AAV1 and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking Signal (TS) ER Export Signal

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    CAG

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pH3U3-mcs

    Plasmid

    #12609

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Multiple cloning site in His3 Ura3 reporter plasmid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLionII

    Plasmid

    #1730

    Purpose

    This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

    Depositor

    Garry Nolan

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2/BH

    Plasmid

    #26556

    Depositor

    Perry Hackett

    Insert

    pT2/BH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Reduced Expression GFP beta actin

    Plasmid

    #31502

    Depositor

    Rick Horwitz, Tim Mitchison

    Article

    Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

    Insert

    beta actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    91-544 of enhancer region of CMV promoter deleted

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PGKneoF2L2DTA

    Plasmid

    #13445

    Depositor

    Philippe Soriano

    Article

    Hoch et al Development. 2006 Feb . 133(4):663-73.

    Insert

    PGKneoF2L2DTA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR223-EGFR

    Plasmid

    #23935

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pGL4 mIL-17 2kb promoter

    Plasmid

    #20124

    Depositor

    Warren Strober

    Article

    Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

    Insert

    mIL-17 (Il17a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TS module

    Plasmid

    #26021

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Tension Sensor module
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pdCas9::BFP-humanized

    Plasmid

    #44247

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 fused to BFP
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS & tagBFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

    Plasmid

    #20943

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available since

    July 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3/Myc-DNMT3L

    Plasmid

    #35523

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3L (DNMT3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBABE-puro-mTwist

    Plasmid

    #1783

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2+hSpCas9

    Plasmid

    #51815

    Purpose

    Encode hSpCas9 for in vitro transcription with Sp6

    Depositor

    Masato Kinoshita

    Article

    Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

    Insert

    humanized S.pyogenes Cas9
    

    Use

    CRISPR; Medaka, zebrafish

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-HA-GASC1

    Plasmid

    #24214

    Purpose

    Mammalian expression of human JMJD2C with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    GASC1 (KDM4C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Rictor_2 shRNA

    Plasmid

    #1854

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBABE YAP1

    Plasmid

    #15682

    Depositor

    Joan Brugge

    Article

    Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    YAP (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HA-HIF2alpha-P405A/P531A-pBabe-puro

    Plasmid

    #19006

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: P405A and P531A

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-Hu-L-Myc

    Plasmid

    #26022

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    L-Myc (MYCL Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pN1 Lck-GCaMP5G

    Plasmid

    #34924

    Purpose

    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

    Depositor

    Baljit Khakh

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    Lck-GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    CMV

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCR-Flag-IKKalpha

    Plasmid

    #15467

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. YFP-tagged FRB (YR)

    Plasmid

    #20148

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FRB (YR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge