20,196 results

Showing: 3681 - 3720 of 20196 results

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SPLICS Po-ER Long P2A

Plasmid

#164113

Purpose

Detect the long Peroxisomes-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP Po-ER Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ii-OVA.I+II

Plasmid

#64596

Purpose

Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Tags

Invariant chain

Expression

Mammalian

Mutation

Only amino acids 242-353 (class I and class II ep…

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEQ176

Plasmid

#83943

Purpose

express beta-galactosidase in eukaryotic cells

Depositor

Adam Geballe

Article

Schleiss et al J Virol. 1991 Dec;65(12):6782-9.

Insert

CMV enhancer/promoter

Tags

beta-galactosidase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60229

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153

Plasmid

#64268

Purpose

GFP-tagged mammalian expression of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

NuP153 (NUP153 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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Emerin pEGFP-C1 (637)

Plasmid

#61993

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS*12-MS2-GFP

Plasmid

#26245

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

MS2-GFP

Expression

Bacterial

Mutation

five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

Available Since

Nov. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRFHUE-eGFP

Plasmid

#89469

Purpose

GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

Depositor

Luis González-Candelas

Article

Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

Insert

eGFP

Use

Binary plasmid for atmt of filamentous fungi

Promoter

A. nidulans gpdA

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.eGFP.WPRE.bGH

Plasmid

#105539

Purpose

AAV expression of EGFP from hSyn promoter

Depositor

James M. Wilson

Has Service

AAV1

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRpHLuorin2-N1

Plasmid

#171716

Purpose

Ratiometric pHLuorin2 backbone (C terminal fusion)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Type

Empty backbone

Expression

Mammalian

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMK444 (EF1-OsTIR1(F74G) Puro)

Plasmid

#214374

Purpose

EF1-OsTIR1(F74G) (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

EF1-OsTIR1(F74G)

Expression

Mammalian

Promoter

EF1 promoter

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TrkB

Plasmid

#32500

Depositor

Rosalind Segal

Insert

TRKB (Ntrk2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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SPYtag-GFPnanobody

Plasmid

#214837

Purpose

This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-GFPnanobody

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ1651-sgTelomere(F+E)

Plasmid

#51024

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

Optimized sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pICH47772

Plasmid

#48004

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-HA-BHLHE40

Plasmid

#110154

Purpose

Expression of human BHLHE40 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-ABE8e-NG

Plasmid

#200447

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn FAK biosensor

Plasmid

#78299

Purpose

FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Lyn Fak biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCIneoEGFP

Plasmid

#46949

Purpose

GFP in Promega pCIneo (uses CMV promoter). Superior to pfwB for some purposes

Depositor

John Schiller

Article

Buck et al Curr Protoc Cell Biol. 2007 Dec;Chapter

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 Y701F

Plasmid

#12302

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 Y701F (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Y701F

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

Plasmid

#64222

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet S2

Plasmid

#171921

Purpose

Express RNA-based (p)ppGpp sensor and a NirFP

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Inserts

RNA-based (p)ppGpp sensor
NirFP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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35S-eGFP-nosT

Plasmid

#80127

Purpose

Transient expression vector for eGFP in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Type

Empty backbone

Tags

eGFP

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
6xHis-SNAP-PI(3,4)P2 Probe

Plasmid

#211511

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

TAPP1 cPHx3

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB401

Plasmid

#74795

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP STAT1 S727E

Plasmid

#12305

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727E (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727E

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT5-bac1.8

Plasmid

#119871

Purpose

Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR'
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

SNP in 592 bp

Promoter

PserA promoter and Pveg promoter with CuO sequence

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PKD1-F (pCI-PKD1-Flag)

Plasmid

#21369

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK229

Plasmid

#71256

Purpose

luciferase reposter with 229 bp of the HSV TK promoter

Depositor

Peter Cockerill

Article

Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

Insert

Thymidine Kinase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

HSV Thymidine Kinase

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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20,196 results