We narrowed to 15,039 results for: NOL

1. pCfB2227

   Plasmid

   #67536

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAs-Cpf1-pGL3-U6-sgRNA

   Plasmid

   #107683

   Purpose

   Expresses AsCpf1 crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNH082

   Plasmid

   #42165

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB2190

   Plasmid

   #67535

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR-P2rP4

   Plasmid

   #141015

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMK390(Stag-3FLAG-BSD)

   Plasmid

   #121191

   Purpose

   C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Stag-3FLAG-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB4786

   Plasmid

   #106142

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G10

    Plasmid

    #67144

    Purpose

    Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    HIS and sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TAL2369

    Plasmid

    #36780

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Right (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TALEN1259

    Plasmid

    #32284

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G03 SP2

    Plasmid

    #67109

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SP2 (SP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pFREE2-xCas9-sgRNA

    Plasmid

    #179579

    Purpose

    Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    xCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBT138b-proA

    Plasmid

    #138514

    Purpose

    P3g

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TALEN1258

    Plasmid

    #32281

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA

    Plasmid

    #64983

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p1.2-Zeo

    Plasmid

    #162740

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBS-IndHom-Tol2-TetR-cryaa-dsRed

    Plasmid

    #73204

    Purpose

    BAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsRed

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    dsRed
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GWF135

    Plasmid

    #133614

    Purpose

    ANR-ANR-BFP-CAAX

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    ANR-ANR-BFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YSBE 596 : pNAT_mCherry

    Plasmid

    #92088

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTMS1

    Plasmid

    #87799

    Purpose

    For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pATD

    Plasmid

    #141012

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCfB2374

    Plasmid

    #67534

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. myc-NLRC5 K234A

    Plasmid

    #37523

    Depositor

    Thomas Kufer

    Article

    Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

    Insert

    NLRC5 (NLRC5 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K234A

    Promoter

    CMV

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pCellFree_G03 USF2

    Plasmid

    #67069

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    USF2 (USF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pBS-TarHom-Tol2-kanaR-cryaa-YFP

    Plasmid

    #74154

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-YFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-zeoCV

    Plasmid

    #73890

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    zeoCV
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJ291_WT_med

    Plasmid

    #128856

    Purpose

    Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

    Depositor

    Mark Pallen

    Article

    Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

    Insert

    Flagellin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMK389 (mCherry-BSD)

    Plasmid

    #121190

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCellFree_G03 TNF

    Plasmid

    #67062

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TNF (TNF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pCellFree_G03 VPS29

    Plasmid

    #67052

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed

    Plasmid

    #73203

    Purpose

    BAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsRed

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    dsRed
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pGEMT-T-HSV2.3 TCR alpha chain

    Plasmid

    #74076

    Purpose

    gBT-I TCR a chain cDNA in pGEM-T

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR alpha clone HSV2.3 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBT100.110

    Plasmid

    #122602

    Purpose

    clone CDA1 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB4796

    Plasmid

    #106131

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJC175e-DddI

    Plasmid

    #179693

    Purpose

    Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTK101

    Plasmid

    #59561

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) mKate-G8-L7Ae-PEST 4xFF4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP

    Plasmid

    #73200

    Purpose

    BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Cerulean

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    CFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGL2B -376/+64

    Plasmid

    #24945

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

44. YSBE 559 : pNAT_6xHA

    Plasmid

    #92087

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    6xHA
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pBW829_pCAG-pExpr-LUT

    Plasmid

    #87554

    Purpose

    Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Id2-Halo donor

    Plasmid

    #113116

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Id2 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCellFree_G05

    Plasmid

    #67139

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His and mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

48. pMM763

    Plasmid

    #68900

    Purpose

    IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    G1104E

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCAG-TetR-Rad18UBD

    Plasmid

    #119010

    Purpose

    Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEF‐PIK3IP1

    Plasmid

    #49214

    Purpose

    expresses PIK3IP1 in mammalian cells

    Depositor

    Lawrence Kane

    Article

    DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

    Insert

    PIK3IP1 (Pik3ip1 Mouse)
    

    Use

    Tags

    6X-his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only