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159,557 results

Showing: 4901 - 4950 of 159557 results

  2. MSCV-opEBNA2-IRES-GFP

    Plasmid
    #220354
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    3 x HA tag
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220354-plasmid-map-sequence-id-437068

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  3. pM-CBE

    Plasmid
    #196293
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding CBE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    196293-plasmid-map-sequence-id-389996

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  5. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52319-plasmid-map-sequence-id-237583

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  6. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  14. pAAV-hSyn-EGFP

    Plasmid
    #50465
    Purpose
    EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50465-plasmid-map-sequence-id-240907

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  21. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

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  22. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid
    #196415
    Purpose
    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smMyc
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and GfaABC1D
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196415-plasmid-map-sequence-id-385068

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  25. pM-ABE

    Plasmid
    #196292
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding ABE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196292-plasmid-map-sequence-id-390025

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  40. pM-mTagBFP2

    Plasmid
    #228615
    Purpose
    Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction
    Depositor
    Marc Birtwistle
    Article
    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.
    Insert
    mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    We introduced some silent mutations to avoid esse…
    Promoter
    CMV
    Available since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228615-plasmid-map-sequence-id-457660

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  41. pCMV-PE7-SB2

    Plasmid
    #228868
    Purpose
    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence
    Depositor
    Sangsu Bae
    Insert
    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    228868-plasmid-map-sequence-id-456310

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  42. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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Showing: 4901 - 4950 of 159557 results