171,784 results
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Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-GFP
Plasmid#58867PurposeAAV expression of GFP under the Syn promoterDepositorInsertGFP
UseAAVExpressionMammalianPromoterSynAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-iGluSnFR4f-NGR-WPRE
Plasmid#234438PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas ServiceAAV1InsertiGluSnFR4f-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
phASCL1-N106
Plasmid#31781DepositorInsertachaete-scute complex homolog 1 (ASCL1 Human)
UseLentiviralAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mNeonGreen2
Plasmid#113727PurposeExpresses the fluorescent protein mNeonGreen2 in a lentiviral backboneDepositorInsertmNeonGreen2
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A
Plasmid#166954PurposeExpresses FLAG-tagged KIF5A protein in pcDNA3.1DepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TMEM63A-peGFP-N1
Plasmid#228590PurposeExpresses mouse TMEM63A-eGFP in mammalian cellsDepositorInsertTMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)
ExpressionMammalianAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8s
Plasmid#162371PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8s
Tags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCMVAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/PD-L1
Plasmid#121478Purposeexpression of human PD-L1 in mammalian cellsDepositorAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRExpressionMammalianPromoterhSynAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3
Plasmid#244092PurposeCytosolic expression of green glucose sensor with non-responsive mRuby3DepositorInsertiGlucoSnFR2.mRuby3
UseAAVTagsmRuby3Available SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Anti-GST [N100/13R ]
Plasmid#188214PurposeMammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13DepositorInsertanti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody
ExpressionMammalianPromoterDual CMVAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PEX14_Pex14
Plasmid#178484PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX208 CMV sTurboID (C)-HA-FRB-ERM
Plasmid#153007PurposeExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membraneDepositorInsertsTurboID (C) (G74)-ERM (Cb5)
UseLentiviralTagsFRB and HAExpressionMammalianPromoterCMVAvailable SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianPromoterdSV40 PromoterAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM234
Plasmid#226656PurposepcDNA3.1-p97-A232E-FLAGDepositorAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_wt p53
Plasmid#22945Purpose3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tagDepositorAvailable SinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2
Plasmid#59904PurposeExpresses 6xHis-Ago2 (N-termi tag) in mammalian cellsDepositorAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3482
Plasmid#144958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMEM63A_OE_1
Plasmid#161667PurposeOverexpresses TMEM63ADepositorAvailable SinceDec. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
DDX5
Plasmid#155523PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-ccdB
Plasmid#14146DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 DOX off blast POLE
Plasmid#241437PurposeExpresses DOX-suppressible POLEDepositorInsertPOLE (POLE Human)
Tags3xFLAGExpressionMammalianMutationSilent Mutations disrupt shRNA targeting sitesAvailable SinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4)P2 Probe
Plasmid#211511PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteriaDepositorInsertTAPP1 cPHx3
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-Chrimson-TS
Plasmid#158702PurposeReporter constructDepositorInsertTRE-mcherry-p2A-Chrimson-TS
UseSynthetic BiologyTagsmCherryExpressionMammalianPromoterTREAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFBR2
Plasmid#116800PurposeLentiviral expression of TGFBR2DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
spMSP1D1
Plasmid#173482PurposeExpresses spMSP1D1 in Bl21DepositorInsertspMSP1D1
TagsHistagExpressionBacterialAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p(A)n-PAUSE
Plasmid#118068PurposeTranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin geneDepositorInsertSynthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ENVLPE+
Plasmid#232427PurposeExpression plasmid to produce ENVLPE+ particlesDepositorInsertENVLPE+
ExpressionMammalianPromoter2xCMVAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-tmpknot-GG-acceptor
Plasmid#174039PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianPromoterhU6Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-3WJdB-T
Plasmid#87308PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.DepositorInsertThree-way junction dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-3HA-SREBP1-C-Flag
Plasmid#134286PurposeLentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1DepositorInsertSREBP1 (Srebf1 Mouse)
UseLentiviralTags3x HA and FlagExpressionMammalianMutationmouse SREBP1 isoform a precursorPromoterEF1aAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMVV052
Plasmid#215303PurposeExpresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPRhaBAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE59
Plasmid#90404PurposeSTAT3 - Sis-inducible element (SIE) gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterSTAT3Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CRISPR_DpmrA
Plasmid#160903PurposeCRISPR_Cas9, lambda red recombineeringDepositorInsertsCas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette
PromoteraraBADAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1
Plasmid#138418PurposeCAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1DepositorInsertdCas9
UseLentiviralTagsBioTAP-tagPromoterEF1aAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ASCL1_P2A_Hygro_Barcode
Plasmid#120427PurposeBarcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-oct4
Plasmid#20323DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSIP403
Plasmid#122028PurposePlasmid for expression of gene of interest in Lactobacillus plantarum and L. sakeiDepositorInsertgusA
ExpressionBacterialPromotersppAAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFlag DVL2 (WT)
Plasmid#24802DepositorAvailable SinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorArticleInsertHalotag-CNA35
TagsHalotagExpressionBacterialAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS9
Plasmid#111762PurposeExpresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS9-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY004 (pcDNA3.1-hFnCpf1)
Plasmid#69976PurposeExpresses humanized FnCpf1DepositorInserthFnCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only