Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

159,134 results

Showing: 4901 - 4950 of 159134 results

  3. Non tagged Avidin

    Plasmid
    #214835
    Purpose
    This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Non tagged Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214835-plasmid-map-sequence-id-423116

    Enlarge

  4. pNLF1-C_PLCG1:1-1291

    Plasmid
    #211089
    Purpose
    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    PLCG1:M1-L1291 (PLCG1 Human)
    Use
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    isoform 2. I813T natural variant (VAR_011908)
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211089-plasmid-map-sequence-id-425967

    Enlarge

  5. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Article
    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

    Enlarge

  9. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

    Plasmid
    #197595
    Purpose
    Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)
    Depositor
    Patrik Verstreken
    Insert
    mRuby2-T2A-GCaMP6f
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hSynI
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197595-plasmid-map-sequence-id-389365

    Enlarge

  16. pQC V5 MCS IRES Puro

    Plasmid
    #110342
    Purpose
    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110342-plasmid-map-sequence-id-218292

    Enlarge

  18. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Promoter
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    108665-plasmid-map-sequence-id-260875

    Enlarge

  20. R777-E160 Hs.PDPK1-nostop

    Plasmid
    #70444
    Purpose
    Gateway ORF clone of human PDPK1 [NM_002613.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PDPK1 (PDPK1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70444-plasmid-map-sequence-id-125893

    Enlarge

  21. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50454-plasmid-map-sequence-id-214300

    Enlarge

  25. TFORF1777

    Plasmid
    #144310
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WHSC1 (NSD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    144310-plasmid-map-sequence-id-471158

    Enlarge

  27. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid
    #223115
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223115-plasmid-map-sequence-id-447640

    Enlarge

  28. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid
    #223116
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223116-plasmid-map-sequence-id-447639

    Enlarge

  32. MSCV-FoxP3-IRES-Thy1.1

    Plasmid
    #229616
    Purpose
    Mammalian expression of full length mouse FoxP3
    Depositor
    Sun Hur
    Insert
    full length FoxP3 (Foxp3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229616-plasmid-map-sequence-id-457336

    Enlarge

  33. MSCV-FoxP3-R337Q-IRES-Thy1.1

    Plasmid
    #229617
    Purpose
    Mammalian expression of full length mouse FoxP3 (R337Q)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R337Q
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229617-plasmid-map-sequence-id-457335

    Enlarge

  34. MSCV-FoxP3-A372S-IRES-Thy1.1

    Plasmid
    #229618
    Purpose
    Mammalian expression of full length mouse FoxP3 (A372S)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A372S
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229618-plasmid-map-sequence-id-457333

    Enlarge

  35. MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

    Plasmid
    #229619
    Purpose
    Mammalian expression of full length mouse FoxP3 (R337Q, A372S)
    Depositor
    Sun Hur
    Insert
    full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R337Q/A372S
    Promoter
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229619-plasmid-map-sequence-id-457331

    Enlarge

  46. TFORF1614

    Plasmid
    #144188
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TADA2B (TADA2B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144188-plasmid-map-sequence-id-404077

    Enlarge

  47. TFORF1434

    Plasmid
    #144175
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKBID (NFKBID Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144175-plasmid-map-sequence-id-404065

    Enlarge

  50. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

    Enlarge

Showing: 4901 - 4950 of 159134 results