167,514 results

1. pBad-PhoCl2c

   Plasmid

   #164035

   Purpose

   Improved PhoCl variant with enhanced dissociation efficiency

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2c
   

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Promoter

   araBad promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA364

   Plasmid

   #215956

   Purpose

   Fragmid fragment: (guide cassette) epegRNA expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dU6:3_v1; BsmBI_v14; BsmBI_v15; evopreQ1_v1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-HA-JMJD2B

   Plasmid

   #24181

   Purpose

   Mammalian expression of human JMJD2B with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2B (KDM4B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a- gfp-2a-puro

   Plasmid

   #129443

   Purpose

   expression of GFP and Puro R

   Depositor

   Oskar Laur

   Insert

   gfp-puro
   

   Use

   Lentiviral

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73022

   Purpose

   Luminescent and fluorescent dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

   Plasmid

   #213119

   Purpose

   Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmGFP-P2A-K(AAA)20-P2A-RFP

   Plasmid

   #105688

   Purpose

   Double fluorescent stalling reporter K(AAA)20

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K(AAA)20
   P2A-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV (from vector)

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS puro HA-hUSP27X

   Plasmid

   #225718

   Purpose

   Bacterial expression of USP27X with HA tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304-KDM6A

   Plasmid

   #203580

   Purpose

   Expresses wild-type KDM6A in mammalian cells.

   Depositor

   William Kaelin, Abhishek Chakraborty

   Article

   Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

   Insert

   KDM6A (KDM6A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-Puro-CLDN18-ARHGAP26

    Plasmid

    #69465

    Purpose

    Expresses Cldn18-Arhgap26 fusion gene.

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    CLDN18-ARHGAP26 (ARHGAP26, CLDN18 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

    Plasmid

    #208692

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSPneoHHgRNAH

    Plasmid

    #63557

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-FLAG-BRAT1

    Plasmid

    #161920

    Purpose

    Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    BRCA1-associated ATM activator 1 (BRAT1 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR5'-attP (pCM254)

    Plasmid

    #68311

    Purpose

    Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

    Depositor

    Leonard Zon, Christian Mosimann

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    attP
    

    Use

    Gateway entry vector

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-mEGFP(A206K)-paCaMKII(K42M)

    Plasmid

    #165440

    Purpose

    Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)
    

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Tags

    mKate

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. RpH-ILV-3xALFA

    Plasmid

    #225135

    Purpose

    RpH-ILV probe strongly colocalises with lysosomal markers.

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. spNW50

    Plasmid

    #173486

    Purpose

    Expresses spNW50 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW50
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. EF.CMV.RFP

    Plasmid

    #17619

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLminP_Luc2P_RE57

    Plasmid

    #90402

    Purpose

    Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    GR

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid

    #117720

    Purpose

    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    mAID-nanobody
    IBB-mCherry
    

    Tags

    importin-beta bining domain (IBB) and triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV and None, IRES-driven

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. VPS35 shRNA2

    Plasmid

    #163859

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRDA_479

    Plasmid

    #179099

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (SpG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TSC2-fl

    Plasmid

    #24940

    Depositor

    Kunliang Guan

    Insert

    TSC2 (Tsc2 Mouse)
    

    Use

    N/a

    Available Since

    Sept. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

28. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

    Plasmid

    #158962

    Purpose

    Cre-dependent Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    hsp70l promoter
    floxed DsRed cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBJ6

    Plasmid

    #167135

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. hprtSAGFP

    Plasmid

    #41594

    Purpose

    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

    Insert

    SA-GFP reporter
    

    Use

    Mouse Targeting; Dsb repair reporter substrate

    Expression

    Mammalian

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3-HA-HA-humanCMYC

    Plasmid

    #74164

    Purpose

    Mammalian expression of human cMyc

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Tags

    2x HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGI3EM20C

    Plasmid

    #135487

    Purpose

    Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr H2B-tdTomato
    

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Promoter

    SpunH2A/Bpr (divergent)

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR223_STAT3_p.A662C_N664C

    Plasmid

    #82184

    Purpose

    Gateway Donor vector containing STAT3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Gateway entry vector

    Mutation

    A662C, N664C

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-ApoE2

    Plasmid

    #197146

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE2 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. HPV16 E6 TCR

    Plasmid

    #122727

    Purpose

    Express HPV16 E6 TCR

    Depositor

    Christian Hinrichs

    Article

    Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

    Insert

    HPV16 E6 TCR
    

    Use

    Retroviral

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPHR-mCherry-hNPM

    Plasmid

    #181887

    Purpose

    Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNPM (NPM1 Mustard Weed, Human)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223_FGF1_WT

    Plasmid

    #81825

    Purpose

    Gateway Donor vector containing FGF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FGF1 (FGF1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET-alpha-synuclein-BiFC

    Plasmid

    #87855

    Purpose

    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    alpha-synuclein-VC155
    alpha-synuclein-VN154
    

    Tags

    C-terminal fragment of mVenus BiFC protein and N-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHAGE-EFS-MCP-3XBFPnls

    Plasmid

    #75384

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLV-beta-catenin deltaN90

    Plasmid

    #36985

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    beta-catenin deltaN90 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90 (Constitutively Acti…

    Promoter

    EF1a

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. SLC25A1_pLX307

    Plasmid

    #98374

    Purpose

    Lentiviral expression of SLC25A1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    SLC25A1 (SLC25A1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET-NtLNtC60αC20C60β

    Plasmid

    #160874

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: pBAD, chaperones: T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEGFPC1-EPSIN 2

    Plasmid

    #22242

    Depositor

    Pietro De Camilli

    Article

    Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

    Insert

    Epsin 2 (EPN2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

47. pPBT-PE2-PuroTK-pegRNA_GG

    Plasmid

    #173222

    Purpose

    piggyBac transposon containing both PE2 and a pegRNA cloning site.

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    PuroTK
    pegRNA cloning cassette
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    CMV, P2A, and hU6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-hSyn-Cre-P2A-dTomato (AAV2)

    Viral Prep

    #107738-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Syn

    Tags

    dTomato (physically separate, not a fusion protein)

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

49. XLone-SOX18

    Plasmid

    #163822

    Purpose

    expresses human SOX18 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    SOX18 (SOX18 Human)
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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