275,537 results
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Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ADAM17-HA
Plasmid#65105PurposeExpression of HA tagged ADAM17 (TACE) in mammalian cellsDepositorAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ADAM17-HA
Plasmid#65105PurposeExpression of HA tagged ADAM17 (TACE) in mammalian cellsDepositorAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
ExpressionMammalianAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
ExpressionMammalianAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRExpressionZebrafishAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRExpressionZebrafishAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianPromoterCBhAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianPromoterCBhAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERmoxGFP
Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorInsertmoxGFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERmoxGFP
Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmoxGFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas serviceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas serviceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct-Cas9
Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
Tags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianPromoterDox inducible Tet-ON promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
Tags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianPromoterDox inducible Tet-ON promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CibN-dCas9-CibN
Plasmid#60553PurposeExpresses CibN-dCas9-CibN in mammalian cellsDepositorInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-CibN-dCas9-CibN
Plasmid#60553PurposeExpresses CibN-dCas9-CibN in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
Available sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-IRES-Blue FP
Plasmid#52115Purposea bicistronic IRES-Blue FP containing retroviral vectorDepositorTypeEmpty backboneUseRetroviralTagsIRES-Blue FPExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-IRES-Blue FP
Plasmid#52115Purposea bicistronic IRES-Blue FP containing retroviral vectorDepositorTypeEmpty backboneUseRetroviralTagsIRES-Blue FPExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#50943PurposeBicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6f
UseAAVPromoterhSyn1Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human Lentiviral sgRNA Library - Nuclear
Pooled library#51047PurposeSub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-EGFP-Tau AP
Plasmid#46905Purposeexpresses EGFP tagged Tau AP in mammalian cellsDepositorInsertTau AP (MAPT Human)
TagsEGFPExpressionMammalianMutationall 14 S/P or T/P amino acid residues (T111, T153…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-EGFP-Tau AP
Plasmid#46905Purposeexpresses EGFP tagged Tau AP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTau AP (MAPT Human)
TagsEGFPExpressionMammalianMutationall 14 S/P or T/P amino acid residues (T111, T153…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-STAT6-IRES-Neo
Plasmid#35482DepositorAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-STAT6-IRES-Neo
Plasmid#35482DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSTAT6 (STAT6 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV500 A-CREB
Plasmid#33371DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA 3 5' cMyc SMC1 wt
Plasmid#32363DepositorPublicationAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA 3 5' cMyc SMC1 wt
Plasmid#32363DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMC1A (SMC1A Human)
Tagsc-MycExpressionMammalianAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-ER beta
Plasmid#28237DepositorAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-ER beta
Plasmid#28237DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertER beta (ESR2 Human)
TagsEGFPExpressionMammalianAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas serviceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas serviceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p-cyclinB1-mcherry (1958)
Plasmid#26063DepositorPublicationAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p-cyclinB1-mcherry (1958)
Plasmid#26063DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCyclin B1 (CCNB1 Human)
TagsmcherryExpressionMammalianAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLM-mCitrine-Sox2
Plasmid#23242DepositorInsertmCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
UseLentiviralAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLM-mCitrine-Sox2
Plasmid#23242DepositorInsertmCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
UseLentiviralAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
TagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChR2-GFP-2A-Halo-YFP
TagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
Plasmid#20299PurposeCre-activated AAV expression of mCherryDepositorHas serviceAAV9InsertmCherry
UseAAVExpressionMammalianAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
Plasmid#20299PurposeCre-activated AAV expression of mCherryDepositorHas serviceAAV9InsertmCherry
UseAAVExpressionMammalianAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV6M-Pak1 K299R
Plasmid#12210DepositorPublicationAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV6M-Pak1 K299R
Plasmid#12210DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPak1 (PAK1 Human)
TagsMycExpressionMammalianMutationCatalytically inactive Pak1: K299RAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR207 SARS-CoV-2 M
Plasmid#141274PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR207 SARS-CoV-2 M
Plasmid#141274PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by