171,723 results
-
Plasmid#225350Purposepiggyback backbone for non-fluorescent GFP tether and H2B-IRFP expressionDepositorInsertH2B (H2B Human, Synthetic)
TagsmiRFP and non-fluorescent GFP (Y67F)-P2AExpressionMammalianAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Puro
Plasmid#214680PurposeLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[R257Q]-P2A-Electra1 (pAH11)
Plasmid#227616PurposeOverexpression of SLC25A46 with R257Q mutation in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
TagsP2A-mElectra1ExpressionMammalianMutationR257QPromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)
Plasmid#227614PurposeOverexpression of SLC25A46 in mammalian cell linesDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
macroH2A2-GFP (pc2191)
Plasmid#223709PurposeExpression of macroH2A2 tagged N-terminal to GFPDepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3-N-GFP
Plasmid#222602PurposeExpression of MAC3-tagged GFP (N-terminal)DepositorInsertEGFP
TagsMAC3ExpressionBacterial and MammalianPromoterCMVAvailable SinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-ArgiNLS-mKate2
Plasmid#220599PurposeConstitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.DepositorInsertmKate2
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-ArgiNLS-EGFP
Plasmid#220601PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent proteinDepositorInsertEGFP
UseAAV and Cre/LoxTagsArgiNLSExpressionMammalianPromoterCAGAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
PSMC3_BirA_Nterm
Plasmid#221524PurposeFlag-tagged biotinylation enzyme for co-expression of Psmc3DepositorAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM9
Plasmid#220167PurposeExpresses a reporter for an alpha-helical OMM proteinDepositorAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_STRN:1-731
Plasmid#211230PurposeMammalian expression of full-length human STRN isoform 2. N-terminal HiBiT tag.DepositorInsertSTRN:M1-V731 (STRN Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationisoform 2PromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOBD2
Plasmid#196826PurposeYeast two-hybrid ; Gal4 DNA binding domainDepositorTypeEmpty backboneTagsGal4 DNA binding domainExpressionYeastPromoterADHAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-SNAPf-mTurquoise2
Plasmid#197497PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in actin filaments.DepositorInsertSNAPf
ExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF17-COMP5AP-AviTag-9xHis
Plasmid#157053PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
bu6-sgCebpa_v1-mU6-sgCebpb_v1-hU6-sgCebpd_v1
Plasmid#177257PurposeExpresses Cebpa_v1 (bU6), Cebpb_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgCebpa_v1/sgCebpb_v1/sgCebpd_v1
UseLentiviralPromoterbU6/mU6/hU6Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_KLF12
Plasmid#167767PurposeExpresses KLF12 in mammalian cellsDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
O-FHL1-1
Plasmid#166543PurposescFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA15c-RFP
Plasmid#126108PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23100Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
PKMYT1 gRNA (BRDN0001146095)
Plasmid#77278Purpose3rd generation lentiviral gRNA plasmid targeting human PKMYT1DepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E340 Hs.STK4-nostop
Plasmid#70624PurposeGateway ORF clone of human STK4 [NM_006282.2] without stop codon (for C-terminal fusions)DepositorInsertSTK4 (STK4 Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
STING-miRFP680
Plasmid#200940PurposeSTING fused with miRFP680 for live cell imagingDepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-HaloTag7-LC3
Plasmid#184899PurposeStable expression of HaloTag7-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
TagsHaloTag7ExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP Cortactin
Plasmid#50728Purposemammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_WNV_PrME
Plasmid#207313PurposeMammalian expression of West Nile Virus PrME proteinDepositorInsertWNV_PrME (POLY West Nile Virus)
ExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-ubi-ASAP1
Plasmid#121987Purposeubiqutously express ASAP1 voltage biosensorDepositorInsertASAP1
UseTol2 transposon destination vectorPromoterzebrafish ubiquitin promoterAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH824-ssr
Plasmid#198027PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Apr resistance cassette
ExpressionBacterialPromoterAprR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
Plasmid#171019PurposeLentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cellsDepositorInsertLargeBit-SNAPtag- Beta-actin
UseLentiviral and LuciferaseTagsBeta actin and LargeBiTExpressionMammalianPromoterEF1Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human Metabolic Gene CRISPRa sgRNA library
Pooled Library#187080PurposeLibrary targeting 2,989 human metabolic genes for activation of gene expression.DepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT56
Plasmid#223428PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TadA8e-PcrA M6
Plasmid#221229PurposeExpress HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.DepositorInsertTadA8e-PcrA M6
ExpressionMammalianAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc CIITA (P#808)
Plasmid#14650DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pREDSIX
Plasmid#74103PurposemCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other AlphaproteobacteriaDepositorTypeEmpty backboneUseConjugative bacterial vectorAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry
Plasmid#166599PurposeEncodes Cre-dependent hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-pSu9-HaloTag7-mGFP
Plasmid#184905PurposeStable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activityDepositorInsertPresequence of Fo-ATPase subunit 9
TagsHaloTag7 and monomeric EGFPExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB402-omega
Plasmid#74797PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneExpressionPlantPromoter2xCaMV35S enhancer and omegaAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Renilla_Luciferase
Plasmid#74444PurposeControl lentiviral vector expressing Renilla luciferaseDepositorInsertRenilla Luciferase
UseLentiviralExpressionMammalianAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TCF1
Plasmid#40620DepositorAvailable SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-HuC-NE2h
Plasmid#208689PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafishDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseCloning vectorPromoterHucAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pTH-iCre-WPREpA
Plasmid#213130PurposeTruncated rat TH promoter expressing iCreDepositorInsertiCre
UseAAV and Mouse TargetingTagsNoneExpressionMammalianPromoterTruncated TH promoter from rat (Rattus norvegicus…Available SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2609
Plasmid#144135PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CD40_WT
Plasmid#82198PurposeGateway Donor vector containing CD40 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG-BACH2
Plasmid#232275PurposeExpression of FLAG-tagged BACH2 (H. sapiens) in human cell linesDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV40-EF1a-hBCL10-SO
Plasmid#75256Purposeretroviral expression vector for expression of StrepOne-Tagged human BCL10DepositorInsertBcl10 (BCL10 Human)
UseRetroviralTagsStrepOne TagExpressionMammalianPromoterpMSCV-LTRs, EF1aAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS1C
Plasmid#55168PurposeEmpty vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
LBR-mCherry
Plasmid#172446PurposeExpression of nuclear envelope protein LBR with a C-terminal mCherry tagDepositorAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)
Viral Prep#83897-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLYS1-FLAG-MitoGFP-HA
Plasmid#50057PurposeExpression of GFP targeted to mitochondria with Flag and HA tagsDepositorInsertMito-GFP
UseLentiviralTagsFlag and HAExpressionMammalianAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro eGFP
Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyExpressionMammalianPromoterTRE3GAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only