163,073 results
-
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC-Pfv-Sapphire_PURO
Plasmid#203651PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry
Plasmid#197420PurposeCas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNADepositorInsertCas9
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV5 trial size)
Viral Prep#50465-AAV5.TPurposeReady-to-use AAV5 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)
Plasmid#197510PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSExpressionMutationABE8e mutations in TadA and nSpRY(D10A/A61R)PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-mCherry
Plasmid#72892PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherryDepositorInserthBACCS2-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 D614G Spike
Plasmid#185692PurposeExpresses codon-optimized full length SARS-CoV-2 D614G SpikeDepositorInsertSARS-CoV-2 D614G Spike (S )
UseTagsExpressionMammalianMutationContains the following mutation: D614GPromoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
FUW-Luc-mCherry-puro
Plasmid#183685PurposeLentiviral vector expressing luciferase and mCherry.DepositorInsertLuc-E2A-mCherry-T2A-PuroR
UseLentiviralTagsExpressionMutationPromoterHuman Ubiquitin C (UbC) promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWT019a
Plasmid#107893PurposeW1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1DepositorInsertPTetO-sd2U-Cas9, PLacO-sgRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)
Viral Prep#162380-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Con/Fon-GCaMP6M (AAV8)
Viral Prep#137119-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EF1a-Con/Fon-GCaMP6M (#137119). In addition to the viral particles, you will also receive purified pAAV-EF1a-Con/Fon-GCaMP6M plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6M. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GCaMP7a
Plasmid#184817PurposeUsed to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).DepositorInsertQUAS:GCaMP7a-pA
UseZebrafish tol2 transgenesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV9)
Viral Prep#100833-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)
Viral Prep#104491-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.MCS.IRES.GFP
Plasmid#169280PurposeConstitutive overexpression of cDNA with GFP as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH1R-P2A-mCherry-N1
Plasmid#84330PurposeCo-expresses (untagged) Histamine-1 receptor and mCherryDepositorInsertH1R-P2A-mCherry (HRH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV Retrograde trial size)
Viral Prep#50465-AAVrg.TPurposeReady-to-use AAV Retrograde trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterSynTagsEGFPAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralTagsExpressionMutationPromoterhEF1aAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-W
Plasmid#211701PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry (AAV PHP.eB)
Viral Prep#114469-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2 (AAV9)
Viral Prep#99130-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDLX5/6TagsmRuby2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
POLD1_pLX307
Plasmid#98358PurposeLentiviral expression of POLD1DepositorInsertPOLD1 (POLD1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR3-CFP
Plasmid#13641DepositorInsertToll-Like Receptor 3 (TLR3 Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV2)
Viral Prep#105537-AAV2PurposeReady-to-use AAV2 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsNoneAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-ChR2-mCherry-Fishell-3
Plasmid#83898PurposeChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertChR2
UseAAVTagsExpressionMammalianMutationPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-N-Ezrin
Plasmid#202684Purposefluorescent protein tagged ezrinDepositorInsertMus musculus EZR(1-586):EGFP (Ezr Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_TEAD2
Plasmid#167804PurposeExpresses TEAD2 in mammalian cellsDepositorInsertTEAD2 (TEAD2 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD
Plasmid#195549PurposedCas12a effector fusion for manipulating transcriptionDepositorInsertLenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD
UseCRISPR and LentiviralTags3XHAExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-AAV-sh[control]
Plasmid#75438Purposecontrol shRNA vectorDepositorInsertGFP
UseAAV and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1h
Plasmid#140557PurposeExpresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA sensor GRAB_rDA1h (high affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opie2-PuroR
Plasmid#131613PurposeExpresses PuroR and dsRed.DepositorInsertsPuroR
dsRed
UseTagsExpressionInsectMutationPromoterOpie2 and hr5Ie1Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Active Wnt4-V5
Plasmid#43812DepositorInsertWNT4 (WNT4 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide
Plasmid#117986PurposepLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.DepositorInsertsgRNA backbone (F+E)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP2SA11
Plasmid#89163PurposeExpress Rotavirus SA11 VP2 in mammalian cellsDepositorInsertRotavirus SA11 VP2
UseTagsExpressionMammalianMutationPromoterT7 promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
APT3-Ykt6
Plasmid#168003Purposepositive selection PACE Accessory PlasmidDepositorInsertYkt6(L161-L177)-T3'PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
DRH337: AAV-hsyn-CheRiff-eGFP
Plasmid#51697PurposeAAV production of CheRiff channelrhodopsin actuatorDepositorInsertCheRiff-eGFP
UseAAVTagseGFPExpressionMutationPromoterhSynapsinAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 Lnp-mCherry
Plasmid#86687PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorInsertLNPK (LNPK Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Circular-oc-CVB3-GFP
Plasmid#226262PurposeProduction of in vitro circularizable RNA via IVTDepositorInsertEGFP
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP1SA11
Plasmid#89162PurposeExpress Rotavirus SA11 VP1 in mammalian cellsDepositorInsertRotavirus SA11 VP1
UseTagsExpressionMammalianMutationPromoterT7 promotorAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3
Plasmid#189755PurposeProduces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet-I3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC3A2
Plasmid#132213PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC3A2 (SLC3A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits