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Showing: 5601 - 5640 of 160711 results

  1. pTfR-mScarlet-I

    Plasmid
    #112954
    Purpose
    In vivo visualization of Transferrin receptors (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Insert
    pTfR
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112954-plasmid-map-sequence-id-327653

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  2. TFORF1062

    Plasmid
    #143751
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC1 (NFATC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143751-plasmid-map-sequence-id-407851

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  8. 8xNFAT-ZsG-muhCD4

    Plasmid
    #162745
    Purpose
    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene
    Depositor
    Maki Nakayama
    Article
    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25
    Insert
    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Human CD4 gene with glutamine to tyrosine at posi…
    Promoter
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    162745-plasmid-map-sequence-id-331322

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  10. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

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  14. pAAV-hDlx-Flex-GFP-Fishell_6 (AAV1)

    Viral Prep
    #83895-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    GFP (Cre-dependent)
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  16. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190034-plasmid-map-sequence-id-371641

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  18. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral Prep
    #121675-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    May 31, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. GPR37-DuET

    Plasmid
    #213282
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR37 (GPR37 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213282-plasmid-map-sequence-id-430904

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  21. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-327840

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  22. pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

    Plasmid
    #196219
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV CAG FLEX iGluSnFR3 v857.PDGFR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196219-plasmid-map-sequence-id-405480

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  29. pNOC_hfnCas12a_CRATES

    Plasmid
    #176251
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitpl
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176251-plasmid-map-sequence-id-347017

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  34. LRP4-Flag/pCDNA3.1

    Plasmid
    #197291
    Purpose
    Plasmid expressing an antigen targeting LRP4 (extracellular)
    Depositor
    Steve Burden
    Insert
    LRP4 (Lrp4 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197291-plasmid-map-sequence-id-388074

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Showing: 5601 - 5640 of 160711 results