167,601 results

1. GST-AR-624-919

   Plasmid

   #104200

   Purpose

   Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

   Depositor

   Elizabeth Wilson

   Article

   He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

   Insert

   human androgen receptor aa 624-919 (AR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Dec. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-TBK1

   Plasmid

   #23851

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Expression

   Mammalian

   Mutation

   Δ40-903

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. S-HBsAg

   Plasmid

   #103013

   Purpose

   Expresses HBV Small envelope glycoprotein in mammalian cells

   Depositor

   Wang-Shick Ryu

   Article

   Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

   Insert

   HBV Small envelope protein
   

   Expression

   Mammalian

   Available Since

   Jan. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Sec61β

   Plasmid

   #172445

   Purpose

   Expression of ER protein Sec61beta with an N-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Protein transport protein Sec61 subunit beta
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Inzolia library in pRDA_550

   Pooled Library

   #209552

   Purpose

   The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

8. pSIRV-NF-kB-eGFP

   Plasmid

   #118093

   Purpose

   Fluorescent reporter for NF-kB activity

   Depositor

   Peter Steinberger

   Article

   Jutz et al Oncotarget. 2017 May 4;8(39):64892-64906

   Insert

   eGFP
   

   Use

   Retroviral

   Promoter

   minimal promoter with NF-kB responsive element

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. shYAP1 # 1

   Plasmid

   #42540

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKL06

    Plasmid

    #104430

    Purpose

    super Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    Super Ecliptic pHluorin-mRuby2
    

    Expression

    Yeast

    Promoter

    Tef1

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-blast-SCRAMBLE

    Plasmid

    #26701

    Purpose

    3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-luciferase

    Plasmid

    #55764

    Purpose

    luciferase reporter for in vivo bioluminescence imaging

    Depositor

    Snorri Thorgeirsson

    Article

    Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

    Insert

    CMV enhancer/chicken beta actin promoter
    

    Expression

    Mammalian

    Promoter

    CMV enhancer/chicken beta actin promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ExRai-CKAR

    Plasmid

    #118409

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT/FLAG/HA-DEST QKI

    Plasmid

    #19891

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    quaking homolog, KH domain RNA binding (QKI Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_hSyn-PdCO-mScarlet-WPRE (AAV5)

    Viral Prep

    #198510-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    Syn

    Tags

    mScarlet

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

17. pFRT-TODestFLAGHA_HuC

    Plasmid

    #65756

    Purpose

    expresses FLAGHA-tagged HuC in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRXIP-GFP-PI4K2A

    Plasmid

    #221760

    Purpose

    Stable expression of GFP-PI4K2A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCZGY546

    Plasmid

    #90466

    Purpose

    Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pmec-4-mcherry
    

    Tags

    mcherry

    Expression

    Worm

    Promoter

    Pmec-4

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Tags

    TurboRFP

    Expression

    Mammalian

    Promoter

    13X repeated p21-p53 response element

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET22b-mEB1-mCherry-6xHis

    Plasmid

    #130984

    Purpose

    Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-mCherry-6xHis (Mapre1 Mouse)
    

    Tags

    mCherry-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA4 HisMax-V5-GFP-RRBP1

    Plasmid

    #92150

    Purpose

    Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 (RRBP1 Human)
    

    Tags

    Calreticulin signal peptide, EGFP (A207K mutation…

    Expression

    Mammalian

    Mutation

    In RRBP1: N951S and T1329L. E1279 is also missing.

    Promoter

    CMV

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFRT-TODestFLAGHA_HuD

    Plasmid

    #65757

    Purpose

    expresses FLAGHA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-DIO-mTurquoise2

    Plasmid

    #104059

    Purpose

    AAV expression of Cre-dependent reporter mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    mTurquoise2
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Active Wnt10B-V5

    Plasmid

    #43823

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT10B (WNT10B Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pWALIUM-intron 3x UAS attB

    Plasmid

    #206360

    Purpose

    includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

    Depositor

    Erdem Bangi

    Article

    Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. AAV-DJ8R

    Plasmid

    #213974

    Purpose

    AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Neurosci Bull. 2023 Jul 11. doi: 10.1007

    Type

    Empty backbone

    Use

    AAV

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMV306hsp

    Plasmid

    #26155

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins

    Pooled Library

    #101929

    Purpose

    Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.

    Depositor

    Michael Bassik

    Article

    Morgens et al Nat Commun. 2017 May 5;8:15178. doi: 10.

    Expression

    Mammalian

    Use

    CRISPR

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.3_eGFP

    Plasmid

    #26822

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-eGFP-3'UTR
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. LARRYv2-EGFP library 1

    Pooled Library

    #233215

    Purpose

    Barcoding pooled library

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

34. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GST-AR-1-660

    Plasmid

    #104196

    Purpose

    Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-660 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA 3.1 myc-Rab10

    Plasmid

    #208366

    Purpose

    Mammalian expression of myc-tagged human Rab10

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10 (RAB10 Human)
    

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-pmEKAR4

    Plasmid

    #174440

    Purpose

    Plasma membrane-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    pmEKAR4
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mEmerald-Calreticulin-N-16

    Plasmid

    #54023

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. spNW25

    Plasmid

    #173484

    Purpose

    Expresses spNW25 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW25
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

    Plasmid

    #160110

    Purpose

    Expresses murine Ifih1 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ifih1 (Ifih1 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5 and V5

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge