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Showing: 7001 - 7040 of 160936 results

  2. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  4. pYPQ202

    Plasmid
    #86198
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis ubiquitin 10 (AtUbi10)
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86198-plasmid-map-sequence-id-174281

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  6. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160730-plasmid-map-sequence-id-357931

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  13. pOpen-P45

    Plasmid
    #165570
    Purpose
    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    P45
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165570-plasmid-map-sequence-id-340824

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  15. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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  18. pAAV-hSyn-JEDI-2P-WPRE (AAV9)

    Viral Prep
    #179464-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-JEDI-2P-WPRE (#179464). In addition to the viral particles, you will also receive purified pAAV-hSyn-JEDI-2P-WPRE plasmid DNA. hSyn-driven expression of the genetically encoded voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Francois St-Pierre
    Article
    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00
    Promoter
    Syn
    Tags
    None
    Available since
    Jan. 24, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. pAcBac1.tR4-MbPyl

    Plasmid
    #50832
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Pyl tRNA cassette
    MbPyl
    two copy Pyl tRNA cassette
    Use
    Baculoviral
    Tags
    Expression
    Insect
    Mutation
    Promoter
    CMV and U6 and H1
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50832-plasmid-map-sequence-id-331231

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  24. AiP13790 - pAAV-AiE0475m-minBG-iCre(R297T)-BGHpA (Alias: CN3790)

    Plasmid
    #230384
    Purpose
    AiE0475m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available since
    Feb. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230384-plasmid-map-sequence-id-462402

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  26. pG108 - K

    Plasmid
    #178039
    Purpose
    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers
    Depositor
    Janina Lewis
    Article
    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1
    Insert
    Kanamycin resistance
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ErmAM has been replaced with Kanamycin for select…
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    178039-plasmid-map-sequence-id-378887

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  29. pLenti6.2_mCherry_ mito_roGFP2-Orp1

    Plasmid
    #155042
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Orp1 (HYR1 Budding Yeast)
    Use
    Lentiviral
    Tags
    mitochondrial targeting sequence and roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155042-plasmid-map-sequence-id-311567

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  31. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41390-plasmid-map-sequence-id-352470

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  39. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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  40. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217342
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217342-plasmid-map-sequence-id-429351

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Showing: 7001 - 7040 of 160936 results