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We narrowed to 13,029 results for: HAL

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Showing: 8751 - 8800 of 13029 results

  7. dCas9-FOG1[N+C]

    Plasmid
    #100085
    Purpose
    dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    FOG1(aa 1-45) (ZFPM1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-FOG1-dCas9-FOG1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100085-plasmid-map-sequence-id-197489

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  8. pNUT N6His Y95F/Y188F hTFNG

    Plasmid
    #69601
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    69601-plasmid-map-sequence-id-124729

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  16. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Article
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  21. pAAV-flex-GFP-shRNA-GluN1

    Plasmid
    #182502
    Purpose
    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Expression
    Mammalian
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available Since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182502-plasmid-map-sequence-id-355962

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  30. FcD265A-FLAG

    Plasmid
    #65420
    Purpose
    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production
    Depositor
    Dane Wittrup
    Article
    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.
    Insert
    FcD265A-FLAG
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D265A
    Promoter
    Modified CMV Promoter
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid
    #182395
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    Tags
    Vesicle Nucleating peptide (VNp) & Leucine Zi…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182395-plasmid-map-sequence-id-364922

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  33. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

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  34. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Mustard Weed, Human)
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103803-plasmid-map-sequence-id-199226

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  38. pCS2+-PIF6-EGFP

    Plasmid
    #154913
    Purpose
    1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    phytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    1-100 amino acids of Arabidopsis PIF6
    Promoter
    CMV
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154913-plasmid-map-sequence-id-308719

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  45. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available Since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84244-plasmid-map-sequence-id-160887

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  46. pOUPc-UL148-mCherry

    Plasmid
    #179279
    Purpose
    All in one "tet-on" lentivirus vector that expresses human cytomegalovirus UL148 fused to an mCherry tag at its cytoplasmic tail. UL148 reorganizes the ER and activates the UPR.
    Depositor
    Jeremy Kamil
    Article
    Zhang et al mBio. 2019 Dec 10;10(6). pii: mBio.02110
    Insert
    UL148 fused to mCherry
    Use
    Lentiviral; Tet-on, all-in-one lentiviral vector
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Tet responsive element 3G
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    179279-plasmid-map-sequence-id-355068

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Showing: 8751 - 8800 of 13029 results