163,409 results

1. pAAV-DIO-Ef1a-TVA-2A-N2cG

   Plasmid

   #172360

   Purpose

   Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CREBBP

   Plasmid

   #38977

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CREBBP (CREBBP Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCR3.1-mIL2

   Plasmid

   #74467

   Purpose

   expression of mouse IL2 in mammalian cells

   Depositor

   Loree Heller

   Article

   Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

   Insert

   IL2 (Il2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCDH-METTL16-3*Flag

   Plasmid

   #196207

   Purpose

   overexpress METTL16

   Depositor

   Jianjun Chen

   Insert

   WT METTL16 (METTL16 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NO

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCVneo-DEST

   Plasmid

   #119746

   Purpose

   Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHB-3

   Plasmid

   #140956

   Purpose

   For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C2-MVP

   Plasmid

   #204543

   Purpose

   Expresses EGFP-tagged MVP in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-mScarlet-EFS-Puro

   Plasmid

   #227259

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-2(KW)2-eGFP

   Plasmid

   #112736

   Purpose

   Express 2(KW)2-eGFP in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-eGFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

13. Strep-KIFC1*-HA

    Plasmid

    #120171

    Purpose

    Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member C1 (KIFC1 Human)
    

    Use

    Tags

    HA and Streptavidin

    Expression

    Mammalian

    Mutation

    Truncated KIFC1: 125-673 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. hygII-UT-ABAleon2.1

    Plasmid

    #106982

    Purpose

    ABAleon2.1 in plant expression vector (Hygromycin resistance)

    Depositor

    Julian Schroeder

    Article

    Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

    Insert

    ABAleon2.1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-KIF1A*-strep

    Plasmid

    #120165

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 1A (Kif1a Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF1A: 1-405 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNC01

    Plasmid

    #233643

    Purpose

    For bacterial expression of sfGFP c-terminally tagged with VitelloTagA.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    sfGFP with c-terminal VitelloTagA
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-GFP [N86/34R]

    Plasmid

    #220419

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCLIP-tag (m) Vector

    Plasmid

    #101136

    Purpose

    Expression of protein of interest with CLIP-Tag in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Type

    Empty backbone

    Use

    Tags

    CLIP-tag (CLIP10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pHAGE-PIK3CA-E542K

    Plasmid

    #116479

    Purpose

    Lentiviral expression of PIK3CA E542K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E542K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti-Tet-GBP1-C589A

    Plasmid

    #138090

    Purpose

    Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Insert

    human GBP1 (GBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    cysteine 589 to alanine (C589A)

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEF1a-Ronin-FLAG-IRES-Neo

    Plasmid

    #28020

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Insert

    Ronin (THAP11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-HA-rat Nrxn1beta AS4(-)

    Plasmid

    #58267

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    see comments

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCEC-red

    Plasmid

    #196040

    Purpose

    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

    Depositor

    Paola Branduardi

    Article

    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Grna cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB6633

    Plasmid

    #106162

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-SpyTag-Flag-SRSF3-V5-mCherry

    Plasmid

    #235091

    Purpose

    SRSF3 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-SpyTag-Flag-SRSF1-V5-mCherry

    Plasmid

    #235089

    Purpose

    SRSF1 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. amilCP_Orange chromoprotein

    Plasmid

    #117850

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Orange
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    64V,65G, BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs-Sox2

    Plasmid

    #13367

    Purpose

    Retroviral expression of mouse Sox2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    SRY-box containing gene 2 (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Tol2 8xSTAR-mScarletI-NLS. PGK-puro

    Plasmid

    #136263

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRVL-5

    Plasmid

    #104583

    Purpose

    Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Human IgG1 CH1/CH2/CH3 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV1)

    Viral Prep

    #198516-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    EF-1a

    Tags

    mScarlet

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

35. pCAG-HA-rat Nrxn2beta AS4(-)

    Plasmid

    #58268

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn2beta AS4(-) (Nrxn2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMCherry F8-2

    Plasmid

    #186034

    Purpose

    Express VHH F8-2 as a mCherry fusion in mammalian cells

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. shMEK1/2 puro

    Plasmid

    #72567

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HTR1B-Tango

    Plasmid

    #66405

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1B (HTR1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Desmoglein2-mCherry

    Plasmid

    #36991

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsg2 (DSG2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid

    #158135

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-Gal4VP64
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge