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167,318 results

Showing: 8951 - 9000 of 167318 results

  4. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #106895
    Purpose
    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106895-plasmid-map-sequence-id-201653

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  6. pAAV-CaMKIIa-mCherry (AAV PHP.eB)

    Viral Prep
    #114469-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  9. R777-E318 Hs.SOS1-nostop

    Plasmid
    #70602
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70602-plasmid-map-sequence-id-126051

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  14. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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  17. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133654-plasmid-map-sequence-id-267529

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  21. R777-E100 Hs.GRB2-nostop

    Plasmid
    #70384
    Purpose
    Gateway ORF clone of human GRB2 [NM_002086.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    GRB2 (GRB2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70384-plasmid-map-sequence-id-125833

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  22. R777-E126 Hs.MAPK1-nostop

    Plasmid
    #70410
    Purpose
    Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70410-plasmid-map-sequence-id-125859

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  23. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Promoter
    LacI-Plac
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  24. pCAGGS-FuG-B2

    Plasmid
    #67513
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.
    Insert
    Fusion Glycoprotein type B2
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Promoter
    CAGGS
    Available Since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67513-plasmid-map-sequence-id-381535

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  32. pVL1393-6xMyc

    Plasmid
    #128035
    Purpose
    Baculovirus expression vector with C-terminal 6xMyc tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    6xMyc tag
    Expression
    Insect
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128035-plasmid-map-sequence-id-254860

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  37. R777-E054 Hs.EGFR-nostop

    Plasmid
    #70338
    Purpose
    Gateway ORF clone of human EGFR [NM_005228.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EGFR (EGFR Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70338-plasmid-map-sequence-id-125787

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  38. R777-E122 Hs.MAP2K1-nostop

    Plasmid
    #70406
    Purpose
    Gateway ORF clone of human MAP2K1 [NM_002755.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70406-plasmid-map-sequence-id-125855

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  39. R777-E214 Hs.RAF1-nostop

    Plasmid
    #70498
    Purpose
    Gateway ORF clone of human RAF1 [NM_002880.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RAF1 (RAF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70498-plasmid-map-sequence-id-125947

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  43. NanoLuc-CCNE1 Vector

    Plasmid
    #238665
    Purpose
    Express NanoLuc-CCNE1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CCNE1 (CCNE1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238665-plasmid-map-sequence-id-481961

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  44. CCNE1-NanoLuc Vector

    Plasmid
    #238666
    Purpose
    Express CCNE1-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CCNE1 (CCNE1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238666-plasmid-map-sequence-id-481962

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  45. LDHA-HiBiT Vector

    Plasmid
    #238587
    Purpose
    Express LDHA-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    LDHA (LDHA Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238587-plasmid-map-sequence-id-478465

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  47. S17-1λpir gyrAR462C

    Bacterial Strain
    #237425
    Purpose
    This engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.
    Depositor
    Xue Liu
    Article
    Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:
    Bacterial Resistance
    None
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 8951 - 9000 of 167318 results