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179,538 results

Showing: 9001 - 9050 of 179538 results

  1. CaMKII-somBiPOLES-mCerulean (AAV9)

    Viral prep
    #154948-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Simon Wiegert
    Publication
    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:
    Promoter
    CaMKIIa(0.4)
    Tags
    mCerulean
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  2. pYPQ-dzCas9-Act3.0

    Plasmid
    #158414
    Purpose
    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158414-plasmid-map-sequence-id-332091

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  6. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid
    #159149
    Purpose
    expresses His-tagged HIV reverse transcriptase and untagged HIV protease
    Depositor
    Andrea Pauli
    Publication
    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d
    Inserts
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    Tags
    His
    Expression
    Bacterial
    Mutation
    HIV-1 group M/subtype B – BH10 strain
    Available since
    Sept. 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    159149-plasmid-map-sequence-id-310074

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  8. pETM33_Nsp5_Mpro

    Plasmid
    #156475
    Purpose
    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Publication
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    156475-plasmid-map-sequence-id-307727

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  15. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128095-plasmid-map-sequence-id-249667

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  16. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    128096-plasmid-map-sequence-id-249669

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  17. pSH-EFIRES-B-AtAFB2-mCherry

    Plasmid
    #129718
    Purpose
    AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry
    Depositor
    Elina Ikonen
    Publication
    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    CRISPR and TALEN; Human safe harbor locus (a avs1…
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    EF1a
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    129718-plasmid-map-sequence-id-254826

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  22. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Mark Arbing
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    124296-plasmid-map-sequence-id-240453

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  26. pcDNA3-ExRai-AktAR

    Plasmid
    #118462
    Purpose
    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AktAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    118462-plasmid-map-sequence-id-230151

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  33. pENN.AAV.CAG.tdTomato.WPRE.SV40 (AAV1)

    Viral prep
    #105554-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.CAG.tdTomato.WPRE.SV40 (#105554). In addition to the viral particles, you will also receive purified pENN.AAV.CAG.tdTomato.WPRE.SV40 plasmid DNA. CAG-driven tdTomato expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CAG
    Tags
    tdTomato
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV5)

    Viral prep
    #100841-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  36. pBad-CA

    Plasmid
    #105665
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105665-plasmid-map-sequence-id-205511

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  41. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    102244-plasmid-map-sequence-id-197289

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  44. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Publication
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  49. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Publication
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    85484-plasmid-map-sequence-id-169864

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Showing: 9001 - 9050 of 179538 results