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179,538 results

Showing: 9151 - 9200 of 179538 results

  4. pMasterK

    Plasmid
    #165081
    Purpose
    Episomal expression of reprogramming factors.
    Depositor
    Hideyuki Okano
    Publication
    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    Use
    Episomal
    Expression
    Mammalian
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    165081-plasmid-map-sequence-id-514763

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  5. pAAV-Ef1a-Con/Fon-oScarlet (AAV8)

    Viral prep
    #137136-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-oScarlet (#137136). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-oScarlet plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of oScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    oScarlet (Cre and Flp-dependent)
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S (AAV5)

    Viral prep
    #106180-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.hSynap-FLEX.SF-iGluSnFR.A184S (#106180). In addition to the viral particles, you will also receive purified pAAV.hSynap-FLEX.SF-iGluSnFR.A184S plasmid DNA. Synapsin-driven, Cre-dependent expression of glutamate sensor iGluSnFR (high affinity). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Promoter
    Syn
    Available since
    March 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-hDlx-Flex-GFP-Fishell_6 (AAV9)

    Viral prep
    #83895-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    GFP (Cre-dependent)
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  14. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral prep
    #112677-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  24. pHR-BRD4ΔN-mCherry-sspB

    Plasmid
    #121968
    Purpose
    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.
    Depositor
    Clifford Brangwynne
    Publication
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    BRD4 (BRD4 Human)
    Use
    Lentiviral
    Tags
    mCherry-sspB
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-461
    Promoter
    SFFV
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    121968-plasmid-map-sequence-id-237552

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  26. mNeonGreen-2A-mTurquoise2

    Plasmid
    #98885
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Publication
    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeonGreen-2A-mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98885-plasmid-map-sequence-id-191168

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  34. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    114404-plasmid-map-sequence-id-220815

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  35. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EJ7-GFP variant of EGFP
    Expression
    Mammalian
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    113617-plasmid-map-sequence-id-218254

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  36. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (AAV1)

    Viral prep
    #98927-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (#98927). In addition to the viral particles, you will also receive purified pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of GFPsm_myc spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Publication
    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:
    Promoter
    CAG
    Tags
    GFPsm-myc (spaghetti monster construct)
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Publication
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Yeast
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    100539-plasmid-map-sequence-id-194476

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  42. pAAV.GfaABC1D.PI.Cre.SV40

    Plasmid
    #105603
    Purpose
    AAV expression of Cre from GfaABC1D promoter
    Depositor
    Baljit Khakh
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    105603-plasmid-map-sequence-id-378896

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  43. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 (AAV5)

    Viral prep
    #98930-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 (#98930). In addition to the viral particles, you will also receive purified pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 plasmid DNA. GFAP-driven glutamate sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Promoter
    GFAP
    Available since
    Feb. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  44. AAV-FLEX-Arch-GFP (AAV1)

    Viral prep
    #22222-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.
    Promoter
    CAG
    Tags
    GFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  47. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)

    Viral prep
    #55645-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only

  48. DNMT3A-dCas9

    Plasmid
    #100090
    Purpose
    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A (DNMT3A Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3A-dCas9-NLS
    Expression
    Mammalian
    Mutation
    includes aa 602-912
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    100090-plasmid-map-sequence-id-197562

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Showing: 9151 - 9200 of 179538 results