167,820 results

1. pLEGFP-WT-STAT3

   Plasmid

   #71450

   Purpose

   Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIPZ shcGAS-Hsa

   Plasmid

   #128175

   Purpose

   Doxycyclin inducible shRNA knockdown of human cGAS gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shcGAS Mmu (CGAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. VRQR-ABEmax

   Plasmid

   #119811

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG014 (RADAR)

   Plasmid

   #157892

   Purpose

   Expresses the RADAR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   RADAR
   

   Expression

   Bacterial

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCT5-bac1.8

   Plasmid

   #119871

   Purpose

   Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

   Depositor

   Claudia Schmidt-dannert

   Article

   Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

   Inserts

   CymR'
   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   SNP in 592 bp

   Promoter

   PserA promoter and Pveg promoter with CuO sequence

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLT3-ITD-MSCV-IRES-GFP

   Plasmid

   #184779

   Purpose

   Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Activating internal tandem duplication (ITD) adde…

   Promoter

   MSCV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. c-Myc-5-HT2A

   Plasmid

   #67944

   Purpose

   N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

   Depositor

   Javier Gonzalez-Maeso

   Article

   Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

   Insert

   HTR2A (HTR2A Human)
   

   Tags

   c-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMDJ39 - Peft-3 Cre (PATCs)

   Plasmid

   #191381

   Purpose

   High efficiency CRE recombinase expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Peft-3 Cre tbb-2 UTR
   

   Expression

   Worm

   Promoter

   Peft-3

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE-60 roGFP2-Orp1-His

    Plasmid

    #64976

    Purpose

    Bacterial expression of His-tagged roGFP2-Orp1

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    His and roGFP2

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD-MSP1D1

    Plasmid

    #161607

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    D-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans were replaced b…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSC-NLS-EGFP

    Plasmid

    #221811

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NLS-EGFP
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-HisD-miniGFP1

    Plasmid

    #188896

    Purpose

    Express 6xHis-Tag miniGFP1 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nrf1-Myc pcDNA3.1(+)-C-Myc

    Plasmid

    #169125

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS314-URA3

    Plasmid

    #11004

    Depositor

    Francisco Malagon

    Insert

    URA3 (URA3 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1 Full length Importin beta

    Plasmid

    #106941

    Purpose

    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

    Insert

    human Importin beta-1 (KPNB1) (KPNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR223_CD40_WT

    Plasmid

    #82198

    Purpose

    Gateway Donor vector containing CD40 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CD40 (CD40 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTRIPZ-hDDX5/17

    Plasmid

    #71307

    Purpose

    Inducible shRNA knockdown of both human DDX5 and DDX17 genes

    Depositor

    Allan Brasier

    Article

    Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

    Insert

    DDX5/17 shRNA (DDX17 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223164

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pCFJ90 - Pmyo-2::mCherry::unc-54utr

    Plasmid

    #19327

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-2::mCherry
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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26. mRuby2-N1

    Plasmid

    #54614

    Purpose

    Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191796

    Purpose

    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13X
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    R84A, H89A, R739A, R740A, H745A, H746A, H747A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pNL [NlucP/STAT6-RE/Hygro] Vector

    Plasmid

    #236925

    Purpose

    Express STAT6 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pMT-puro

    Plasmid

    #17923

    Purpose

    Insect expression vector

    Depositor

    David Sabatini

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Drosophila metallothionein gene promoter drives e…

    Expression

    Insect

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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31. CRISPR-StAR4BN (Lenti)

    Plasmid

    #222692

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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32. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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33. pLenti-pHluorin_M153R-CD63

    Plasmid

    #172117

    Purpose

    Fluorescent reporter for CD63-positive exosome secretion

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    pHluorin_M153R in a small extracelluar loop of CD…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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35. AAV-hSyn-Soma-jGCaMP8s (AAV1)

    Viral Prep

    #169256-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from AAV-hSyn-Soma-jGCaMP8s (#169256). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8s plasmid DNA. hSyn-driven expression of soma-targeted calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

36. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid

    #178209

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.C.HA and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNC-Cam1304-RNAi

    Plasmid

    #193399

    Purpose

    Nimble cloning-compatible vector for plant RNAi

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLG015 (RADAR)

    Plasmid

    #157893

    Purpose

    Expresses the RADAR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RADAR subtype containing auxillary gene rdrD
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA6.2 EmGFP hAQP4(M23)

    Plasmid

    #126464

    Purpose

    Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Aquaporin 4 (AQP4 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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