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173,479 results

Showing: 10251 - 10300 of 173479 results

  5. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229851-plasmid-map-sequence-id-460711

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  12. mTagRFP-T2-Tubulin-6

    Plasmid
    #58044
    Purpose
    Localization: Microtubules, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 31, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58044-plasmid-map-sequence-id-218690

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  13. Pv-RNR-R2

    Plasmid
    #25589
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-RNR-R2
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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  23. pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #162382-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  27. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Christoph Bock
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219680-plasmid-map-sequence-id-462884

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  28. pLV-REPORT(PGK/CMV)

    Plasmid
    #172330
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK/CMV
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172330-plasmid-map-sequence-id-497743

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  30. TFORF3348

    Plasmid
    #144824
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PITX2 (PITX2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144824-plasmid-map-sequence-id-391553

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  35. T4 Lysozyme WT*

    Plasmid
    #18111
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    No cysteines: C54T, C97A
    Available Since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18111-plasmid-map-sequence-id-174143

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  36. pLenti-CMV-Pink Flamindo-p2a-bPAC

    Plasmid
    #202716
    Purpose
    Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Inserts
    Pink Flamindo
    bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    202716-plasmid-map-sequence-id-402843

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  50. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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Showing: 10251 - 10300 of 173479 results