167,514 results

1. pX330-PITCh-TUBA1B

   Plasmid

   #207763

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_BiVIPe4_ChR2_mCherry

   Plasmid

   #213856

   Purpose

   AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   Channelrhodopsin 2-mCherry
   

   Use

   AAV

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Floxed-STOP mCherry

   Plasmid

   #122963

   Purpose

   mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_Floxed-STOP mCherry
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MLRV

   Plasmid

   #118069

   Purpose

   Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Inserts

   TB:5xNF-κβ:RedF:bGHpA
   TB:4xTGF-β:FLuc:bGHpA
   TB:5xE-box:Renilla:bGHpA
   TB:2xp53:NLuc:bGHpA
   TB:6xAP-1:GrRenilla:bGHpA
   CMV:ELuc:bGHpA
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Expression

   Mammalian

   Promoter

   2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Glycogene CRISPR Library

   Pooled Library

   #140961

   Purpose

   Targets human genes involved in cellular glycosylation.

   Depositor

   Sriram Neelamegham

   Article

   Zhu et al Glycobiology. 2020 Aug 8. pii: 5889976.

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

6. pMAL-c2X

   Plasmid

   #75286

   Purpose

   Expresses proteins in the cytoplasm as fusions to maltose-binding protein

   Depositor

   Paul Riggs

   Article

   Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

   Type

   Empty backbone

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 18, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAH01

   Plasmid

   #128162

   Purpose

   IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

   Depositor

   Calvin Henard, Michael Guarnieri

   Article

   Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. H3K9me3Probe_FullLength

   Plasmid

   #186463

   Purpose

   Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

   Depositor

   Chongli Yuan

   Article

   Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

   Insert

   dimeric CDY1 (CDY1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP

   Plasmid

   #165830

   Purpose

   EGFP green fluorescent protein CDS

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   EGFP
   

   Expression

   Insect

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-Sos1

    Plasmid

    #32920

    Depositor

    Dafna Bar-Sagi

    Article

    Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

    Insert

    SOS1 (SOS1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

    Pooled Library

    #75312

    Purpose

    Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV8)

    Viral Prep

    #105547-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pPB-cT3G-cERP2-NR4A1

    Plasmid

    #192925

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR4A1 (NR4A1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSDest2

    Plasmid

    #22424

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Type

    Empty backbone

    Use

    Destination vector

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF-5xT7-TtCsm

    Plasmid

    #128572

    Purpose

    For expression of T. thermophilus Csm in E. coli (Csm1-5)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Csm1
    Csm2, Csm3, Csm4, Csm5
    

    Tags

    HRV 3C protease cleavage site and 10xHis tag on C…

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli and Cod…

    Promoter

    T7 promoter, lac operator

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. BL21ΔF

    Bacterial Strain

    #102259

    Purpose

    ompF deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pVL1393-6xMyc

    Plasmid

    #128035

    Purpose

    Baculovirus expression vector with C-terminal 6xMyc tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    6xMyc tag

    Expression

    Insect

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLP-hyg

    Plasmid

    #87831

    Purpose

    Expresses FLP recombinase with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    heat-shock inducible

    Available Since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Brm WT-Flag pShuttle

    Plasmid

    #122105

    Purpose

    Expression of full length Brm in mammalian cells

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    BRM (SMARCA2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Expression

    Bacterial

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVoPo-mCh

    Plasmid

    #187841

    Purpose

    Constitutive mCherry-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSFFV_mNG3K(1-10)

    Plasmid

    #157993

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G27D, D42E, M128I, K153M, T170I

    Promoter

    SFFV

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti X1 Puro DEST (694-6)

    Plasmid

    #17297

    Purpose

    3rd gen lentiviral promoter-less Gateway destination vector, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. epi-BE4max

    Plasmid

    #135975

    Purpose

    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-FLAG-KD LKB1

    Plasmid

    #8591

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K78I: lysine 78 mutated to isoleucine; kinase dead

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV.GFA104.PI.eGFP.WPRE.bGH (AAV5)

    Viral Prep

    #100896-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.GFA104.PI.eGFP.WPRE.bGH (#100896). In addition to the viral particles, you will also receive purified pAAV.GFA104.PI.eGFP.WPRE.bGH plasmid DNA. GFA104-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Philip Haydon

    Article

    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi

    Promoter

    GFA104 (GFAP promoter)

    Tags

    EGFP

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

29. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMD1 anti-GFP nanobody-Tn5

    Plasmid

    #215567

    Purpose

    Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

    Depositor

    Isabelle Mansuy

    Article

    Maria A. et al bioRxiv 2024.07.12.603224

    Insert

    anti-GFP nanobody-Tn5 fusion
    

    Tags

    3xFlag and intein-CBD

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TS module

    Plasmid

    #26021

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Tension Sensor module
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAWPSG-Po-nCas9-BE

    Plasmid

    #195740

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Po (phoenol-inducible promoter)

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

    Plasmid

    #172067

    Purpose

    Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pInducer20 ALPK1 Y254C

    Plasmid

    #231587

    Purpose

    ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed tyrosine 254 to cysteine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

    Plasmid

    #40810

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(31-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

37. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-ninein

    Plasmid

    #73519

    Purpose

    Encodes mouse ninein tagged with an EGFP tag at its N-terminus

    Depositor

    Michel Bornens

    Article

    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

    Insert

    ninein (Nin Mouse)
    

    Tags

    EGFP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pT7-NSP4SA11

    Plasmid

    #89171

    Purpose

    Express Rotavirus SA11 NSP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP4
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MAC-DPP4

    Plasmid

    #172416

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Dipeptidyl peptidase 4 (DPP4 Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFL

    Plasmid

    #110739

    Purpose

    Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Polyhedrin and p10 promoters

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLV-EgI-HygroR

    Plasmid

    #207177

    Purpose

    Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pJFRC7-20XUAS-IVS-mCD8::GFP

    Plasmid

    #26220

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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