268,891 results
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p663-UBC-miniTurbo-V5-NEK6_IDG-K
Plasmid#189872PurposeGateway destination clone of NEK6 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK6 (NEK6 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB426
Plasmid#68691PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB426
Plasmid#68691PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMitoLOC
Plasmid#58980PurposeExpression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrilaDepositorInsertsmCherry
GFP
UseTagspreCOX4 and preSU9ExpressionYeastMutationPromoterADH1 and MET17Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMitoLOC
Plasmid#58980PurposeExpression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrilaDepositorInsertsmCherry
GFP
UseTagspreCOX4 and preSU9ExpressionYeastMutationPromoterADH1 and MET17Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-p150Glued
Plasmid#36154DepositorInsertp150Glued (DCTN1 Human)
UseTagsEGFPExpressionMammalianMutationsee documentationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-p150Glued
Plasmid#36154DepositorInsertp150Glued (DCTN1 Human)
UseTagsEGFPExpressionMammalianMutationsee documentationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3117
Plasmid#144593PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMZF1 (MZF1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3117
Plasmid#144593PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMZF1 (MZF1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Sox2AV-T2A-Klf4-E2A-Myc
Plasmid#210017Purposeepisomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.DepositorUseTagsExpressionMammalianMutationSox2A61V is a point mutant of Sox2 that enhances …PromoterCAGAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
TARDBP_Halo_N_allele
Plasmid#178131PurposeDonor vector for endogenous tagging of human TARDBP at the N-terminus with halotagInsertHalotag (TARDBP Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-ABEmax-BlastR
Plasmid#152989PurposeA-to-G base editorDepositorInsertABE7.10-nSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-ABEmax-BlastR
Plasmid#152989PurposeA-to-G base editorDepositorInsertABE7.10-nSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SypHer mt
Plasmid#48251PurposepH sensor, targeted to mitochondriaDepositorInsertSypHer
UseTags2 mitochondrial targeting sequencesExpressionMammalianMutationC199SPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
SypHer mt
Plasmid#48251PurposepH sensor, targeted to mitochondriaDepositorInsertSypHer
UseTags2 mitochondrial targeting sequencesExpressionMammalianMutationC199SPromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
p405MET25
Plasmid#15961DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
enIscB-T5E
Plasmid#205411PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
UseTagsExpressionMammalianMutationE85R+H369R+S387R+S457RPromoterCAG, hU6, CMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0429
Plasmid#143213PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPBX1 (PBX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0429
Plasmid#143213PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPBX1 (PBX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast
Plasmid#205550PurposeExpress EPN24-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/TO/V5-DEST-IGFBP2-Clover
Plasmid#191008PurposeLentiviral expression vector for IGFBP2 with a fluorescent Clover tagDepositorInsertInsulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
UseLentiviralTagsCloverExpressionMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV044
Plasmid#191824PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-CHMP2B
Plasmid#115329Purposeexpresses mEGFP-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mEGFP-CHMP2B
Plasmid#115329Purposeexpresses mEGFP-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_His2-GFP11
Plasmid#153511PurposeHis2-GFP11 C-terminal Tag Donor PlasmidDepositorInsertHis2-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_His2-GFP11
Plasmid#153511PurposeHis2-GFP11 C-terminal Tag Donor PlasmidDepositorInsertHis2-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6_(Gluc)_INT(GenPurpClon)
Plasmid#68433PurposeGeneral Purpose cloning vector for "INT"-like constructs under U6-promoter expression.DepositorInsertINT construct
UseCRISPR; Cloning vector for generating int-like co…TagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(Gluc)_INT(GenPurpClon)
Plasmid#68433PurposeGeneral Purpose cloning vector for "INT"-like constructs under U6-promoter expression.DepositorInsertINT construct
UseCRISPR; Cloning vector for generating int-like co…TagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-CAAX
Plasmid#116856Purposeplasma membrane markerDepositorInsertHRAS
UseTagsTagBFP2ExpressionMammalianMutationAmino acids 172-189PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-CAAX
Plasmid#116856Purposeplasma membrane markerDepositorInsertHRAS
UseTagsTagBFP2ExpressionMammalianMutationAmino acids 172-189PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TMED9-bio-His
Plasmid#51855PurposeExpresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED9 (TMED9 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TMED9-bio-His
Plasmid#51855PurposeExpresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED9 (TMED9 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CFP
Plasmid#13030PurposeMammalian expression vector for cloning your gene fused to CFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CFP
Plasmid#13030PurposeMammalian expression vector for cloning your gene fused to CFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTK645
Plasmid#114711PurposeExpress NuMA(1700-2115)-RFP-Nano from Rosa 26 locusDepositorInsertNuMA(1700-2115)-RFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK645
Plasmid#114711PurposeExpress NuMA(1700-2115)-RFP-Nano from Rosa 26 locusDepositorInsertNuMA(1700-2115)-RFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPDK3876(TRV-RNA2-pPEBV-MCS)
Plasmid#149275PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAsDepositorInsertModified TRV RNA2 with PEBV promoter
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPDK3876(TRV-RNA2-pPEBV-MCS)
Plasmid#149275PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAsDepositorInsertModified TRV RNA2 with PEBV promoter
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
