Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Applied Filters

20+ requests Clear filter

20,807 results

Showing: 11401 - 11450 of 20807 results

  6. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

    Enlarge

  22. pGR

    Plasmid
    #46002
    Purpose
    Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose
    Depositor
    Christopher Voigt
    Article
    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    June 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    46002-plasmid-map-sequence-id-64680

    Enlarge

  26. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Kathleen Collins
    Insert
    PP7 coat protein optimized
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Available Since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28174-plasmid-map-sequence-id-13350

    Enlarge

  31. mEos3.2-Lifeact-7

    Plasmid
    #54696
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Lifeact-mEos3.2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54696-plasmid-map-sequence-id-137746

    Enlarge

  34. MSP1-bio

    Plasmid
    #47709
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47709-plasmid-map-sequence-id-68315

    Enlarge

  35. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV5)

    Viral Prep
    #112005-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Tags
    mRuby3
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only

  40. CCR5-Tango

    Plasmid
    #66242
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CCR5 (CCR5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  41. EGFP-hCaM

    Plasmid
    #47602
    Depositor
    Emanuel Strehler
    Insert
    CaM (CALM3 Human)
    Tags
    EGFP-HA-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47602-plasmid-map-sequence-id-231168

    Enlarge

  46. HK2

    Plasmid
    #25529
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HK2 (HK2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pTwist-SARS-CoV-2 Δ18 B.1.1.529

    Plasmid
    #179907
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    179907-plasmid-map-sequence-id-351282

    Enlarge

  50. pRK GFP Paxillin

    Plasmid
    #50529
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    Paxillin (PXN Human)
    Tags
    GFP21
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50529-plasmid-map-sequence-id-203086

    Enlarge

Showing: 11401 - 11450 of 20807 results