We narrowed to 15,977 results for: LEA

1. SeeSaw Reporter (SSR1.0)

   Plasmid

   #50392

   Purpose

   SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

   Depositor

   Pablo Huertas Sánchez

   Article

   Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

   Insert

   SSR1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211841

   Purpose

   Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA and mCherry

   Expression

   Plant

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Dendra2-LaminB1-10

   Plasmid

   #57728

   Purpose

   Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   Dendra2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. PEP110E

   Plasmid

   #97396

   Purpose

   binary construct of nuclear mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Tags

   Nucleus target

   Expression

   Plant

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4046814

   Plasmid

   #145601

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. BbZIP-cALFA

   Plasmid

   #160498

   Purpose

   For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   BbZIP
   

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME-nlsGFP-P2A

   Plasmid

   #80808

   Purpose

   nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   minimal cytomegalovirus immediate early enhancer/promoter
   

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-REPORT(PGK)-12xHRE

   Plasmid

   #172334

   Purpose

   hypoxic responsive reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. V1-MESA-45F-M-tTA

   Plasmid

   #84500

   Purpose

   MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTA

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V1-MESA-45F-M-tTA
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJOG536

    Plasmid

    #105423

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Nuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST14-SpyCatcher002 EQ

    Plasmid

    #102830

    Purpose

    Bacterial expression of unreactive version of SpyCatcher002

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002 EQ
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    E77Q mutation prevents isopeptide bond formation

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGem-nHyPer2:sHyper2

    Plasmid

    #84737

    Purpose

    Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Tags

    NLS-SV40

    Expression

    Plant

    Promoter

    pFMV/p35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046754

    Plasmid

    #145647

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046747

    Plasmid

    #149249

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SP6-hCas9-Ce-mRNA

    Plasmid

    #47911

    Purpose

    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

    Depositor

    Paul Sternberg

    Article

    Chiu et al Genetics. 2013 Aug 26.

    Insert

    hCas9 with C. elegans 5' and 3' UTRs
    

    Use

    CRISPR

    Promoter

    SP6

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046733

    Plasmid

    #145665

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pmScarlet-i_NES_C1

    Plasmid

    #85062

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCB11

    Plasmid

    #201111

    Purpose

    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

    Depositor

    Mary Dunlop

    Article

    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

    Inserts

    CcaR
    tetA(C)
    sfGFP
    

    Expression

    Bacterial

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    J23100 and PcpcG2-172

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGBW-m4046795

    Plasmid

    #145598

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pGBW-m4046910

    Plasmid

    #145672

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pISH-NQO1

    Plasmid

    #136766

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Nqo1

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Nqo1 (Nqo1 Mouse)
    

    Use

    In situ probe

    Mutation

    ISH Probe insert, V184A- Please see depositor co…

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGBW-m4046740

    Plasmid

    #145588

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. mPapaya-Nup50-N-10

    Plasmid

    #56348

    Purpose

    Localization: Nuclear Pore, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Nup50 (NUP50 Human)
    

    Tags

    mPapaya

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBW-m4046809

    Plasmid

    #145685

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGBW-m4046916

    Plasmid

    #145627

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pAAV- CAG-h2BmRuby2FLAG

    Plasmid

    #116872

    Purpose

    Nuclear localized mRuby2FLAG

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    H2BmRuby2FLAG
    

    Use

    AAV

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMEH280 NLS dTomato 3x high affinity MS2 loops

    Plasmid

    #168211

    Purpose

    Mammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGBW-m4046871

    Plasmid

    #145724

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pCS-nlstdTomato

    Plasmid

    #132985

    Purpose

    full length of nuclear localized tdTomato in pCS expression vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    tdTomato
    

    Tags

    NLS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGBW-m4046731

    Plasmid

    #145633

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGBW-m4046833

    Plasmid

    #145649

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pEGFP-N1-mt-ro2GFP

    Plasmid

    #82408

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pQE80L TriCatcher-RGDSP

    Plasmid

    #112631

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central RGDSP integrin-binding sequence

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. H2A-mTurquoise-RHOG-QL-deltaCAAX

    Plasmid

    #176096

    Purpose

    Constitutively active, nuclear localized RhoG

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOG (RHOG Human)
    

    Expression

    Mammalian

    Mutation

    QL

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Tags

    HA and NLS

    Expression

    Insect

    Promoter

    tubulin

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGBW-m4046806

    Plasmid

    #145746

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pKL1-222

    Plasmid

    #219409

    Purpose

    Nuclear expression of Synrophin PDZ clamp protein

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    pTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
    

    Expression

    Yeast

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCDNA-FRT-FAK-kinase30

    Plasmid

    #59124

    Purpose

    FAK fret control sensor without FERM domain with 30 nm linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pHisIHFαIHFβ

    Plasmid

    #149384

    Purpose

    Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Inserts

    Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
    Pseudomonas aeruginosa PA14 IHFbeta
    

    Tags

    6xHis tag and HRV3C cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pUE1-TSP-ORF68

    Plasmid

    #162650

    Purpose

    Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pVRC8400-SARS-CoV-2-RBD-BA.4/5-AVI

    Plasmid

    #192114

    Purpose

    Expresses SARS-CoV-2 RBD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Insert

    SARS-CoV-2-RBD-BA.4/5 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

50. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only