179,553 results
-
Pooled library#86538DepositorAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
ExpressionBacterialPromoterPF6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pF CAG luc-EGFP-cre puro
Plasmid#67503PurposeConstitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cellsDepositorInsertluciferase-EGFP-cre
UseCre/Lox, Lentiviral, and Luciferase ; Fluorescent…ExpressionMammalianPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBK-EQR-BE3
Plasmid#85172PurposeExpresses EQR-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEQR-BE3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
ExpressionBacterial and MammalianPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2 mCherry-Atl1
Plasmid#86678PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCRISPRi-v2, Membrane Proteins (m6), top 5 sgRNAs/gene
Pooled library#83994PurposeMouse CRISPRi Pooled Library targeting membrane proteinsDepositorAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene
Pooled library#83972PurposeHuman CRISPRi Pooled Library targeting cancer and apoptosis genesDepositorAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
ExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RAS mutant clone collection
Plasmid kit#1000000089PurposeGateway entry clones containing expression vectors for RAS protein mutants for in vitro and in vivo research.DepositorApplicationGene Expression and LabelingVector typeMammalian ExpressionAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 NLS1, 5F-L YFP
Plasmid#84913PurposeMammalian expresion of TDP-43 NLS1, 5F-L YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTDP-43 (TARDBP Human)
TagsYFPExpressionMammalianMutationK82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…PromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
SRC_HUMAN_D0
Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorPublicationInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hsgk1
Plasmid#83432Purposefull length human sgk1 with Flag tagDepositorAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
TagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-
pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTag-BFP-C-h-Rab11a
Plasmid#79805PurposeExpress TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab11a (RAB11A Human)
TagsTagBFPExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-V1-ORF
Plasmid#73635PurposeGateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).DepositorTypeEmpty backboneTagsVenus fragment 1 (V1)ExpressionMammalianPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNICKclos2.0
Plasmid#73228PurposeGenome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9 nickase
sgRNA to xylR
UseE.coli - clostridium shuttle vectorMutationD10APromoterPj23119 and PthlAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 91-710
Plasmid#75140PurposeExpresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 91-710PromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZW1-FCS-circRNA
Plasmid#73449PurposeExpression of circular RNADepositorAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIP-VEGFA121
Plasmid#73017PurposeRetroviral expression of human VEGFA121DepositorAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DH-BB(5'SIN;TE12ORF)
Plasmid#72309PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virusDepositorInsertDH-BB(5'SIN;TE12ORF)
UseSindbis virus helper constructAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-EF1-DIO-tdTomato
Plasmid#72254PurposeExpress tdTomato under EF-1 promoter when Cre is expressed by another vectorDepositorInserttdTomato
UseCre/Lox and LentiviralExpressionMammalianPromoterelongation factor-1Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGZ108 (pFA6a-3FLAG-MNase-kanMX6)
Plasmid#70231PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GRM2-Tango
Plasmid#66388PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRM2 (GRM2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CXCR2-Tango
Plasmid#66260PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR2 (CXCR2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CXCR7-Tango
Plasmid#66265PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR7 (ACKR3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTK305
Plasmid#66812PurposeIVT vector for Fluc mRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFirefly luciferase
UseLuciferase; Mrna ivtPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJH46
Plasmid#67272PurposeConjugally transferable red recombinogenic plasmidDepositorInserttraJ, cat, oriT
UseRed recombinasePromotercat gene promoter from PGNS-BACAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GalToxBFP
Plasmid#68073Purposemammalian expression of GC localized oxBFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGalT signal anchor transmembrane domain
TagsmoxBFPExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Prig-3::CD4::spGFP1-10
Plasmid#65829PurposeFor membrane contactDepositorPublicationInsertHomo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
TagsspGFP1-10ExpressionWormPromoterPrig-3Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRC_RR_VP1_r1c3
Plasmid#65724PurposeRescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSRDepositorInsertmCherry
UseAAVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERoxBFP
Plasmid#68126Purposemammalian expression of ER localized oxBFPDepositorInsertoxBFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINS-UBE3C
Plasmid#66711PurposeExpresses UBE3C HECT domain in E. coliDepositorAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-SEP-Glur2 (MCA10)
Plasmid#64941Purposemammalian expression of rat GluR2DepositorAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPCX-IRES-GFP
Plasmid#65436PurposeFor transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.DepositorInsertIRES-GFP
UseRetroviralExpressionMammalianPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPCX-Cx43-IRES-GFP
Plasmid#65433PurposeMammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral productionDepositorAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-Tight-Puro_Sox10
Plasmid#46536PurposeA lentiviral vector for the expression of mouse Sox10 under doxycyline control.DepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGDestTol2LC-3sgRNA
Plasmid#64241Purposedestination vector for three U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGDestTol2LC-2sgRNA
Plasmid#64240Purposedestination vector for two U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-pLKO-puro-SOX2
Plasmid#47540PurposeTet inducible knockdown of SOX2.DepositorInsertSOX2 shRNA
UseLentiviral and RNAiExpressionMammalianPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQHA-USP7 WT puroR
Plasmid#46753PurposeRetroviral vector that expresses wild type HA-tagged human USP7DepositorAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOS001: CMV-Optopatch2_FCK
Plasmid#62984PurposeCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoterDepositorInsertOptopatch2
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mDsRed-Golgi-7
Plasmid#55832PurposeLocalization: Golgi, Excitation: 557, Emission: 585DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGolgi (B4GALT1 Human)
TagsmDsRedExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Spectrin-C-cpstFRET
Plasmid#61111PurposeExpression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fieldsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspectrin-cpstFRET
ExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only