We narrowed to 22,490 results for: SCR

1. NylCp2-TS_pET28a

   Plasmid

   #215421

   Purpose

   Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D122G/H130Y/D36A/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRDA_078

   Plasmid

   #158582

   Purpose

   Lentiviral expression of BE4max and customizable gRNA

   Depositor

   David Root, John Doench

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZR060_CROPseq-sgCtrl-CRISPRi

   Plasmid

   #180269

   Purpose

   Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   No-Target Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA-FOXO3a WT

   Plasmid

   #1787

   Depositor

   Michael Greenberg

   Article

   Brunet et al Cell 1999 Mar 19;96(6):857-68.

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. actc1b-Lifeact-mKate2

   Plasmid

   #109493

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-Lifeact-mKate2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SaCas9

   Plasmid

   #188494

   Purpose

   Expresses FLAG tagged SaCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ141B2.0

   Plasmid

   #99897

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-KLF5

   Plasmid

   #40900

   Depositor

   Jin-Tang Dong

   Article

   Chen et al Oncogene. 2002 Sep 26;21(43):6567-72.

   Insert

   Kruppel-like factor 5 (KLF5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PCMV

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 Flag FKHR

    Plasmid

    #13507

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    FKHR (FOXO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1A-Dm-miR-92a

    Plasmid

    #22767

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

    Insert

    micro RNA 92a (mir-92a Fly, primary transcript)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Expression

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBRα-β flap (831-1057)

    Plasmid

    #53734

    Purpose

    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    alpha NTD-Beta flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Use

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_501

    Plasmid

    #96922

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAMBIA1301-RmMYB108

    Plasmid

    #173180

    Purpose

    Overexpresses Rosa multiflora MYB108 in plants

    Depositor

    Daidi Che

    Article

    Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

    Insert

    RmMYB108
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDA_256

    Plasmid

    #158581

    Purpose

    Lentiviral expression of BE3.9max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCas9-EcRT-Z3-Hyg

    Plasmid

    #232095

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xIRS luciferase

    Plasmid

    #13511

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    3xIRS (IGFBP1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EcNusGFL

    Plasmid

    #113122

    Purpose

    Expresses full-length E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZR069_CROPseq-sgPTPRC-CRISPRi

    Plasmid

    #180271

    Purpose

    Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    PTPRC (CD45) sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-PKCA WT

    Plasmid

    #195202

    Purpose

    Mammalian expression vector for GFP tagged PKCA.

    Depositor

    Peter Parker, David Bryant

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. tdTomato-LacI

    Plasmid

    #183921

    Purpose

    Lac repressor labeled with tdTomato; can be used to label lacO sites

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdTomato-LacI (lacI Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-hG9a

    Plasmid

    #33025

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    hG9a (EHMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRDA_118

    Plasmid

    #133459

    Purpose

    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BirA-Myc_N_TAL1

    Plasmid

    #167728

    Purpose

    Expresses TAL1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    TAL1 (TAL1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-ReaChR-citrine

    Plasmid

    #50956

    Purpose

    ReaChR-citrine in lentival transfer vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    Lentiviral

    Tags

    citrine

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXD70LacZ1-promoterless-LacZ

    Plasmid

    #191617

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-FOXB1

    Plasmid

    #153112

    Purpose

    Expression of Flag tagged human FOXB1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXB1 (FOXB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK2020

    Plasmid

    #171932

    Purpose

    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

    Depositor

    Judith Kimble

    Article

    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

    Insert

    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    

    Use

    Tags

    superfolder GFP, mCherry

    Expression

    Worm

    Mutation

    Promoter

    -

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Flag-FOXA3

    Plasmid

    #153111

    Purpose

    Expression of Flag tagged human FOXA3 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXA3 (FOXA3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cherry-ICP0FxE-C3

    Plasmid

    #134562

    Purpose

    Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)

    Depositor

    Susan Janicki

    Article

    Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

    Insert

    ICP0
    

    Use

    Tags

    Cherry

    Expression

    Mammalian

    Mutation

    RING finger deletion (FXE)

    Promoter

    CMV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag-FOXB2

    Plasmid

    #153113

    Purpose

    Expression of Flag tagged human FOXB2 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXB2 (FOXB2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. T7RNAP Cyslight

    Plasmid

    #205077

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight (T7p07 Bacteriophage T7)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LentiV2-mU6-evopreQ1

    Plasmid

    #210190

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYPQAT59

    Plasmid

    #223431

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTRIP CMV GFP1-9

    Plasmid

    #130271

    Purpose

    Lentiviral expression vector for stable expression of GFP1-9

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    GFP 1-9 OPT
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Flag-FOXL1

    Plasmid

    #153134

    Purpose

    Expression of Flag tagged human FOXL1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXL1 (FOXL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLNCX2-mCherry-VPS4A

    Plasmid

    #115334

    Purpose

    expresses mCherry-VPS4A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation in position 4050 C to T

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge