283,910 results

1. Gal:mCherry-uTEV3

   Plasmid

   #135453

   Purpose

   Yeast expression of uTEV3 fused to mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-uTEv3
   

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   S219V mutation improves stability , I138T/S153N/T…

   Promoter

   Gal

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 JellyOp

   Plasmid

   #41432

   Depositor

   Robert Lucas

   Article

   Bailes et al PLoS One. 2012;7(1):e30774. Epub 2012 Ja

   Insert

   Box jellyfish opsin
   

   Tags

   1D4 epitope at 3' end

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-GFAP-iGABASnFR2-WPRE (AAV5)

   Viral Prep

   #218871-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pGP-AAV-GFAP-iGABASnFR2-WPRE (#218871). In addition to the viral particles, you will also receive purified pGP-AAV-GFAP-iGABASnFR2-WPRE plasmid DNA. GFAP-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Promoter

   GFAP

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

4. dCas9-dMSK1

   Plasmid

   #165602

   Purpose

   Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 S. Pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL278_P23-DsRed-Express2

   Plasmid

   #121505

   Purpose

   E. coli-streptococcal shuttle vector that expresses rfp

   Depositor

   Robert Shields

   Article

   Shields et al Appl Environ Microbiol. 2019 May 17. pii

   Insert

   DsRed-Express2
   

   Use

   E. coli streptococcal shuttle vector

   Promoter

   P23

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Dre-T2A-TagRFP

   Plasmid

   #167968

   Purpose

   Transient expression of Dre and TagRFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   Dre recombinase
   

   Tags

   TagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAX APP C99

   Plasmid

   #30146

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   APP C99 (APP Human)
   

   Expression

   Mammalian

   Mutation

   C-terminal from BACE site of APP

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-puro-cMyc

   Plasmid

   #46970

   Purpose

   lentiviral expression of c-Myc

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUtdTW

    Plasmid

    #22478

    Depositor

    Connie Cepko

    Article

    Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    tandem dimer tomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. SypHer

    Plasmid

    #48250

    Purpose

    pH sensor

    Depositor

    Nicolas Demaurex

    Article

    Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

    Insert

    SypHer
    

    Expression

    Mammalian

    Mutation

    C199S

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pJMK001

    Plasmid

    #33780

    Depositor

    Adam Cohen

    Article

    Kralj et al Science. 2011 Jul 15;333(6040):345-8.

    Insert

    Proteorhodopsin Optical Proton Sensor
    

    Expression

    Bacterial

    Mutation

    D97N

    Promoter

    AraBAD

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. METTL3 shRNA 2 tet pLKO puro

    Plasmid

    #162984

    Purpose

    Tet-inducible shRNA targeting human METTL3 #2

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Lentiviral

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ERK-KTR-mStrawberry reporter

    Plasmid

    #158686

    Purpose

    This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLYS1-FLAG-MitoGFP-HA

    Plasmid

    #50057

    Purpose

    Expression of GFP targeted to mitochondria with Flag and HA tags

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    Mito-GFP
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pD649-HAsp-CEACAM1-COMP5AP-AviTag-9xHis

    Plasmid

    #157390

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CEACAM1 (CEACAM1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSGKP-km

    Plasmid

    #117233

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. GW1-pHRed

    Plasmid

    #31473

    Purpose

    genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Expression

    Mammalian

    Available Since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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26. ER-SPLICSs-cFAST10

    Plasmid

    #210431

    Purpose

    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    ER-SPLICSs-cFAST10
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. mCh-Mfn2

    Plasmid

    #141156

    Purpose

    Mammalian expression and labeling mitofusin 2

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 2 (MFN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLV-REPORT(PGK/CMV)-12xHRE

    Plasmid

    #172335

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK/CMV

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. AAV-EF1a-BbChT (AAV9)

    Viral Prep

    #45186-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Promoter

    EF1a

    Tags

    mCherry, mTFP, or none

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

31. pmEmerald-ERGIC-53

    Plasmid

    #170717

    Purpose

    Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pMAL-XPG

    Plasmid

    #39215

    Depositor

    Aziz Sancar

    Article

    Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

    Insert

    XPG (ERCC5 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Note that XPG starts at amino acid 5

    Promoter

    Ptac

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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33. pET28a-uvsX-H6

    Plasmid

    #163913

    Purpose

    Expresses uvsX for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsX
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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34. pMF230

    Plasmid

    #62546

    Purpose

    Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

    Depositor

    Michael Franklin

    Article

    Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

    Insert

    GFP mut2
    

    Use

    Broad host range plasmid

    Promoter

    trc

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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35. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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36. pTRE3G-EGFP-IRES(Neo)R

    Plasmid

    #158068

    Purpose

    eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    eGFP
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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37. pDestattB (pCM268)

    Plasmid

    #68313

    Purpose

    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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38. pEGFP-hTipin (pc4732)

    Plasmid

    #216862

    Purpose

    Mammalian expression vector encoding GFP tagged human Tipin

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Tipin (TIPIN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mEmerald-Vimentin-C-18

    Plasmid

    #54300

    Purpose

    Localization: Intermediate Filaments, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pHIV-dTomato

    Plasmid

    #21374

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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