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169,450 results

Showing: 11361 - 11400 of 169450 results

  2. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197024
    Purpose
    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized OsCas12f1
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197024-plasmid-map-sequence-id-388957

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  7. pV1393

    Plasmid
    #111430
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111430-plasmid-map-sequence-id-212561

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  16. pWS158

    Plasmid
    #90517
    Purpose
    Cas9 gap repair vector - URA3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90517-plasmid-map-sequence-id-287206

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  18. phage UbiC scAB-GFP

    Plasmid
    #104998
    Purpose
    Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    single chain Antibody against GCN4
    Use
    Lentiviral
    Tags
    GB1, HA tag, and sfGFP
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104998-plasmid-map-sequence-id-202897

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  21. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

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  24. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid
    #136445
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-717). Codon-optimize…
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136445-plasmid-map-sequence-id-269879

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  28. pAAV.hSyn.eGFP.WPRE.bGH (AAV1)

    Viral Prep
    #105539-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.eGFP.WPRE.bGH (#105539). In addition to the viral particles, you will also receive purified pAAV.hSyn.eGFP.WPRE.bGH plasmid DNA. Synapsin-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pNuc-TrxRFP1

    Plasmid
    #98998
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and nuclear localization sequence
    Tags
    His-tag and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98998-plasmid-map-sequence-id-190366

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  34. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid
    #188383
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    Use
    Lentiviral
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188383-plasmid-map-sequence-id-371054

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  36. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid
    #136453
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136453-plasmid-map-sequence-id-271682

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  39. TFORF1863

    Plasmid
    #143327
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU2F2 (POU2F2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143327-plasmid-map-sequence-id-391414

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Showing: 11361 - 11400 of 169450 results