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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 128,551 results for: CIL

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Showing: 41 - 60 of 128551 results

  3. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid
    #12457
    Purpose
    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.
    Depositor
    Randall Moon
    Publication
    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mutant TCF/LEF binding sites (Ctnnb1 )
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutant TCF/LEF binding sites
    Available since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12457-plasmid-map-sequence-id-322378

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  8. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61425-plasmid-map-sequence-id-457486

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  9. hCas9

    Plasmid
    #41815
    Purpose
    Expresses human codon optimized Cas9 nuclease for genome engineering
    Depositor
    George Church
    Publication
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41815-plasmid-map-sequence-id-106044

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  12. pCMV-PE2

    Plasmid
    #132775
    Purpose
    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See manuscript
    Promoter
    CMV
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132775-plasmid-map-sequence-id-259720

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  13. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid
    #62987
    Purpose
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
    Depositor
    Feng Zhang
    Publication
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9n-2A-Puro
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 D10A nickase mutant
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62987-plasmid-map-sequence-id-435258

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  14. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41393-plasmid-map-sequence-id-364440

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  15. pAAV2/9n

    Plasmid
    #112865
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap9
    Use
    AAV
    Promoter
    Truncated P5
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112865-plasmid-map-sequence-id-350300

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  17. C. difficile toxin B mRNA toehold switch sensor

    Plasmid
    #110715
    Purpose
    Toehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output
    Depositor
    James Collins
    Publication
    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    C. difficile toxin B toehold switch sensor
    Use
    Synthetic Biology
    Promoter
    T7
    Available since
    Sept. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    110715-plasmid-map-sequence-id-217849

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  18. pcDNA3 APEX2-NES

    Plasmid
    #49386
    Purpose
    Expresses APEX2 in the cytosol of mammalian cells
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flag - APEX2 - NES
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K14D, W41F, E112K, A134P on soybean APX; improved…
    Promoter
    CMV
    Available since
    Nov. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49386-plasmid-map-sequence-id-327822

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Showing: 41 - 60 of 128551 results