179,463 results
-
Plasmid#78264PurposeLentiviral empty cloning vector containing IRES and GFPDepositorTypeEmpty backboneUseLentiviralTagsIRES-EGFPExpressionMammalianPromoterCMV, lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pAVU6+27-F30-2xdBroccoli
Plasmid#66842PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
ExpressionMammalianPromoterU6Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-P4M-SidMx2
Plasmid#51472PurposeBiosensor for PtdIns4PDepositorPublicationInsertsP4M-SidM(546-647)
P4M-SidM(546-647)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-P4M-SidM
Plasmid#51471PurposeBiosensor for PtdIns4PDepositorPublicationInsertP4M-SidM(546-647)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCalN-ddGFP-A
Plasmid#40290DepositorAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-Jmjd3-F
Plasmid#17440PurposeExpression of Jmjd3 in mammalian cellsDepositorAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-beta-catenin-S33Y
Plasmid#13371PurposeRetroviral expression of mouse beta-catenin S33Y mutantDepositorInsertcatenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)
UseRetroviralExpressionMammalianMutationSerine 33 to Tyrosine and Leucine 388 to ProlineAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLAG/HA-DGCR8
Plasmid#10921DepositorAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIreSneo-FLAG/HA Ago3
Plasmid#10823DepositorAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1236 pcDNA3 Myr HA Akt3
Plasmid#9017DepositorAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas serviceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-ChR2(H134R)-GFP (AAV8)
Viral prep#58880-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-ChR2(H134R)-GFP (#58880). In addition to the viral particles, you will also receive purified pAAV-Syn-ChR2(H134R)-GFP plasmid DNA. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG1
Plasmid#80795PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotypeDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRMC2
Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…ExpressionBacterialAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
TagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
Plasmid#50460PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR - EGFP sgRNA 1
Plasmid#51760PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 1
UseCRISPR and LentiviralExpressionMammalianPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
Plasmid#51083PurposeCre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas serviceAAV RetrogradeInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C321.ΔA
Bacterial strain#48998PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)DepositorBacterial resistanceAmpicillinSpeciesEscherichia coliAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-
TS module
Plasmid#26021DepositorInsertTension Sensor module
ExpressionMammalianAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
N-Myc-53BP1 WT pLPC-Puro
Plasmid#19836DepositorAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL43.1 - Pglh-2::MosTase::glh-2utr
Plasmid#19332DepositorPublicationInsertPglh-2::Mos1 transposase
ExpressionWormAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD44S pBabe-puro
Plasmid#19127DepositorPublicationAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCyPet-His
Plasmid#14030DepositorInsertCyPet
Tags6xHisExpressionBacterialAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cdk4-HA
Plasmid#1876DepositorAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
413 pSG5L HA RB
Plasmid#10720DepositorAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV9)
Viral prep#83898-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsmCherryAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH (AAV PHP.eB)
Viral prep#105550-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven expression of Cre. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-ACE2
Plasmid#141185PurposeMammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tagDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-mCh-Cry2WT
Plasmid#101221PurposeBlue-light dependent optogenetic protein Cry2DepositorInsertCry2PHR
UseLentiviralTagsmCherryExpressionMammalianPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS DRR mCherry Donor EF1a BFP
Plasmid#98896PurposeHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.DepositorInsertNeomycin-T2A-mCherry-GFP
UseSynthetic BiologyExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
Tags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianPromoterCMVAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
TagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
4XCLEAR-luciferase reporter
Plasmid#66800PurposeReporter for activity of the CLEAR networkDepositorInsert4XCLEAR
UseLuciferasePromoterFour CLEAR elements (GTCACGTGAC) in tandem derive…Available sinceNov. 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPCX cyto Grx1-roGFP2
Plasmid#64975PurposeMammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)DepositorAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC119-gRNA
Plasmid#52255PurposeCan use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.DepositorInsertguide RNA targeting AtPDS3 (PDS3 Mustard Weed)
UseCRISPR; Plant expressionPromoterArabidopsis U6 polymerase III promoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
wtTDP43tdTOMATOHA
Plasmid#28205DepositorPublicationAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BirA in pET28a (w400-2)
Plasmid#26624DepositorInsertBirA
TagsHisExpressionBacterialAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2XMyc-LRRK2-WT
Plasmid#25361DepositorPublicationAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-DsRed Express
Plasmid#22724DepositorInserta variant of Discosoma sp. red fluorescent protein
UseRetroviralExpressionMammalianMutationN/AAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEGFP-HRas G12V
Plasmid#18666DepositorAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
multi-shRNA vector
Plasmid#12391DepositorPublicationAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK792
Plasmid#8830DepositorInsertTEV protease, S219P mutant
TagsHis, MBP (cleaved), and polyarginineExpressionBacterialMutationS219P mutationAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE hBRM
Plasmid#1961DepositorPublicationAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by