Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

284,094 results

Showing: 2001 - 2050 of 284094 results

  13. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

    Enlarge

  14. cr3Ec

    Plasmid
    #184860
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184860-plasmid-map-sequence-id-363182

    Enlarge

  22. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Britta Eickholt
    Article
    An et al PLoS One. 2015 Dec 30;10(12):e0145783. d
    Insert
    PTEN (PTEN Human)
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Available Since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86781-plasmid-map-sequence-id-169199

    Enlarge

  23. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Britta Eickholt
    Article
    An et al PLoS One. 2015 Dec 30;10(12):e0145783. d
    Insert
    PTEN (PTEN Human)
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Available Since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86781-plasmid-map-sequence-id-169199

    Enlarge

  34. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

    Enlarge

  36. p406MET3

    Plasmid
    #17436
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET3 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17436-plasmid-map-sequence-id-8610

    Enlarge

  37. p406MET3

    Plasmid
    #17436
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET3 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17436-plasmid-map-sequence-id-8610

    Enlarge

  38. pLenti.puro.MCV.ER.RAZ.2

    Plasmid
    #114382
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.
    Depositor
    Reety Arora, Sudhir Krishna
    Article
    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    MCV NCCR (viral own promoter)
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114382-plasmid-map-sequence-id-221614

    Enlarge

  39. pLenti.puro.MCV.ER.RAZ.2

    Plasmid
    #114382
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.
    Depositor
    Reety Arora, Sudhir Krishna
    Article
    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    MCV NCCR (viral own promoter)
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114382-plasmid-map-sequence-id-221614

    Enlarge

  42. pD649-HAsp-TEK-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156957
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TEK (TEK Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156957-plasmid-map-sequence-id-382014

    Enlarge

  43. pD649-HAsp-TEK-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156957
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TEK (TEK Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156957-plasmid-map-sequence-id-382014

    Enlarge

  45. PEAR1-bio-His

    Plasmid
    #51860
    Purpose
    Expresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PEAR1 (PEAR1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51860-plasmid-map-sequence-id-79749

    Enlarge

  46. PEAR1-bio-His

    Plasmid
    #51860
    Purpose
    Expresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PEAR1 (PEAR1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51860-plasmid-map-sequence-id-79749

    Enlarge

  47. MAGEA4

    Plasmid
    #39001
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA4 (MAGEA4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39001-plasmid-map-sequence-id-218943

    Enlarge

  48. MAGEA4

    Plasmid
    #39001
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA4 (MAGEA4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39001-plasmid-map-sequence-id-218943

    Enlarge

Showing: 2001 - 2050 of 284094 results