166,779 results
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Plasmid#17442PurposeDestination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reactionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only
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pAAV-syn-flex-iGluSnFR4s-NGR-WPRE (AAV1)
Viral Prep#234441-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-flex-iGluSnFR4s-NGR-WPRE (#234441). In addition to the viral particles, you will also receive purified pAAV-syn-flex-iGluSnFR4s-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4s-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
The Human Druggable CRISPR library
Pooled Library#200013PurposeThe library contains ~10,000 sgRNAs targeting 1,980 human genes (5 sgRNAs per gene and 100 nontargeting control sgRNAs).DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFR WT
Plasmid#11011PurposeRetroviral construct for expressing human EGFR in human cellsDepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified) (AAV PHP.S)
Viral Prep#59462-PHP.SPurposeReady-to-use AAV PHP.S particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHP.S serotype, which permits efficient transduction of the peripheral nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (codon diversified)Available SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-WT
Plasmid#17608PurposeMammalian expression of HA tagged ubiquitinDepositorAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
AiP12251-pAAV-AiE0453m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2251)
Plasmid#164453PurposeAiE0453m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neuronsDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
UseTagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoGFP
Plasmid#44385DepositorInsertmitoGFP (COX8A Human)
UseLentiviralTagsCox8 targeting sequence and GFPExpressionMammalianMutationPromoterCMVAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW62.YY1.FKBP.knock-in.mCherry
Plasmid#104370PurposeFor knocking in FKBP F36V P2A mCherry onto YY1DepositorInsertFKBP12(F36V) -P2A-mCherry
UseTagsExpressionMutationPromoterAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6s.WPRE.SV40 (AAV5)
Viral Prep#100843-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.Syn.GCaMP6s.WPRE.SV40 (#100843). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA
Plasmid#163480PurposeDirect-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpADepositorInserttTA2
UseAAVTagsExpressionMutationPromoterBeta Globin minimal promoterAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5_mCherry_P2AT2A_GFP-nb-2xKS
Plasmid#238236PurposeFor overexpression of mCherry_P2AT2A_GFP-nb-2xKSDepositorInsertmCherry_P2AT2A_GFP-nb-2xKS
UseTagsExpressionMammalianMutationPromoterAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3'3xFlag
Plasmid#208616PurposeEmpty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlagDepositorTypeEmpty backboneUseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
UseTagsExpressionMammalianMutationPromoterU6Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 puro
Plasmid#98290PurposeVariant of lentiCRISPRv2 that confers puromycin resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe4_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213944-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiSSTe4_dTomato_nlsdTomato (#213944). In addition to the viral particles, you will also receive purified pAAV_BiSSTe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the SST interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM-mStayGold
Plasmid#234317Purposecodon-optimized mStayGold with synthetic introns for the expression in C. elegansDepositorInsertC. elegans codon optimized mStayGOld
UseTagsmStayGoldExpressionWormMutationPromoterAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-HTG
Plasmid#27437DepositorInserthistone-GFP-2A-TVA receptor-2A-rabies glycoprotein
UseAAV; Adeno-associated viralTagsExpressionMutationN/APromoterAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP-LC3ΔG
Plasmid#84572PurposeExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable SinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
UseTagsExpressionMammalianMutationPromoterCAGAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2max-BSD
Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralTagsExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP (Concentrated Lentiviral Prep)
Viral Prep#61422-LVCPurposeReady-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.DepositorPromoterEF1aTagsNoneAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries
Pooled Library#1000000228DepositorAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA1er
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
UseTagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLXIN-Luc
Plasmid#60683Purposestable expresses luciferase in mammalian cells by retrovirus systemDepositorInsertFirefly Luciferase
UseRetroviralTagsExpressionMutationPromoterAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiFUCCI(CA)5
Plasmid#223176PurposeLentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)DepositorInsertFUCCI(CA)5
UseLentiviralTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionMammalianMutationPromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorInsertRfx-Cas13d
UseCRISPRTagsHAExpressionMutationPromoterAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-4mtD3cpv
Plasmid#36324DepositorInsert4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
UseTagsFirst 29 aa of human COX subunit 8aExpressionMutationPromoterCMVAvailable SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-EGFP
Plasmid#80605PurposeMammalian expression vector that expresses HIV-1 GAG with EGFP fused to itDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterSRalphaAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-B19G (AAV1)
Viral Prep#100799-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.DepositorPromoterTREtightTagsBFP (in presence of Cre and 100798)Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Blast DEST (w524-1)
Plasmid#19065Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, BlastDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorTagsExpressionMutationPro2Ala, possibly Asp613Gly and changes made to A…PromoterAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (AAV9)
Viral Prep#108912-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (#108912). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 plasmid DNA. CAG-driven, Cre-dependent ChroME-ST-P2A-H2B-mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3 (Cre-dependent)Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP1122
Plasmid#104159PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BP1-2 pLPC-Puro
Plasmid#19835DepositorInsertFragment of p53-Binding Protein 1 (TP53BP1 Human)
UseRetroviralTagsmCherryExpressionMutationFragment of human 53BP1 aa 1220 - 1711PromoterAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral Prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sybody Generation Toolbox
Plasmid Kit#1000000160PurposeThe Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.DepositorAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pArk313
Plasmid#200257PurposeArk313 rep/capDepositorInsertArk313 Rep/Cap
UseAAVTagsExpressionMutationPromoterAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-PBX1
Plasmid#170699Purposedoxycycline-inducible overexpression of PBX1DepositorInsertPBX1 (PBX1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_526
Plasmid#184859PurposeLentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only