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We narrowed to 14,447 results for: cas9 genes

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Showing: 2281 - 2320 of 14447 results

  3. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

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  5. p300 core-dCas9-CBP core

    Plasmid
    #179559
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179559-plasmid-map-sequence-id-354771

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  8. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  13. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179558-plasmid-map-sequence-id-354773

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  15. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

    Plasmid
    #209298
    Purpose
    A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.
    Depositor
    Birgitt Schuele
    Article
    Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1
    Inserts
    dCas9
    tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    tetracycline-inducible promoter (TRE3G)
    Available Since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    209298-plasmid-map-sequence-id-414164

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  17. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  19. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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  20. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-492918

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  23. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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  32. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

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  38. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68345-plasmid-map-sequence-id-438782

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Showing: 2281 - 2320 of 14447 results