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Showing: 2441 - 2480 of 160228 results

  2. pAAV-hSyn-DIO-EGFP (AAV Retrograde)

    Viral Prep
    #50457-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-CAG-GFP (AAV Retrograde trial size)

    Viral Prep
    #37825-AAVrg.T
    Purpose
    Ready-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. pAK_DR30_CasRx_GFP

    Plasmid
    #134843
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    134843-plasmid-map-sequence-id-266705

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  8. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV.CMV.ffLuciferase.SV40 (AAV8)

    Viral Prep
    #105532-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.ffLuciferase.SV40 (#105532). In addition to the viral particles, you will also receive purified pAAV.CMV.ffLuciferase.SV40 plasmid DNA. CMV-driven ffLuciferase control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    ffLuciferase
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pFLAG_WT MDA5

    Plasmid
    #216798
    Purpose
    Expresses wild type (wt) melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216798-plasmid-map-sequence-id-428831

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  19. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid
    #26730
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26730-plasmid-map-sequence-id-334693

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  21. Lck-mScarlet-I

    Plasmid
    #98821
    Purpose
    In vivo visualization of the plasma membrane (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lck
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98821-plasmid-map-sequence-id-358010

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  23. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  30. pAAV-GFAP-hM4D(Gi)-mCherry (AAV5)

    Viral Prep
    #50479-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP-hM4D(Gi)-mCherry (#50479). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM4D(Gi)-mCherry plasmid DNA. GFAP-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    GFAP
    Tags
    mCherry
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)

    Viral Prep
    #137128-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  35. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Promoter
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  37. M13cp-dg3 hp

    Plasmid
    #218095
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218095-plasmid-map-sequence-id-432463

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  40. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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Showing: 2441 - 2480 of 160228 results