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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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178,032 results

Showing: 3151 - 3200 of 178032 results

  3. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available Since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    40306-plasmid-map-sequence-id-115990

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  5. CMV-G-GECO1.1

    Plasmid
    #32445
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    G-GECO1.1
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32445-plasmid-map-sequence-id-150291

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  13. T4 Lysozyme WT

    Plasmid
    #18110
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Available Since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18110-plasmid-map-sequence-id-398030

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  24. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral Prep
    #104492-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. pRK5-myc-Rac1-wt

    Plasmid
    #12985
    Depositor
    Gary Bokoch
    Insert
    Rac1 (RAC1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12985-plasmid-map-sequence-id-217442

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  40. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Expression
    Mammalian
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  43. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV PHP.eB)

    Viral Prep
    #100854-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only

  44. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

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  46. pAAV-Ef1a-DIO EYFP (AAV Retrograde)

    Viral Prep
    #27056-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, Cre-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Tags
    EYFP (Cre-dependent)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV-hsyn-flex-Voltron-ST (AAV1)

    Viral Prep
    #119036-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Article
    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641
    Promoter
    Syn
    Available Since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 3151 - 3200 of 178032 results