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Showing: 3251 - 3300 of 19980 results

  10. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  11. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  13. pAAV-Ef1a-fDIO mCherry (AAV8)

    Viral Prep
    #114471-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only

  24. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

    Plasmid
    #89347
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    Jurkat TCRalpha/beta alleles and CD3e and CD3z
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89347-plasmid-map-sequence-id-176322

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  27. pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV8)

    Viral Prep
    #65014-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    Syn
    Tags
    GFP
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. pmal_c5x_RNAse_Inhib

    Plasmid
    #153314
    Purpose
    Bacterial expression of an RNase inhibitor
    Depositor
    Philippa Marrack, Drew Endy
    Insert
    RNAse Inhibitor
    Use
    Tags
    Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153314-plasmid-map-sequence-id-298771

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  31. pDisplay-mgSrtA

    Plasmid
    #125792
    Purpose
    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
    Depositor
    P. Chen
    Article
    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18
    Insert
    mgSrtA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125792-plasmid-map-sequence-id-258287

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  33. pAAV.GFAP.Cre.WPRE.hGH (AAV5)

    Viral Prep
    #105550-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    GFAP
    Tags
    None
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. p11-LacY-wtx1

    Plasmid
    #69056
    Purpose
    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.
    Depositor
    Huimin Zhao
    Article
    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15
    Inserts
    ccdB (ccdB )
    lacY (lacY )
    Use
    Tags
    A177C
    Expression
    Mutation
    Promoter
    pBAD and pLac
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69056-plasmid-map-sequence-id-372941

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  39. pCS-kI

    Plasmid
    #51553
    Purpose
    Mammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired.
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    phiC31 integrase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    51553-plasmid-map-sequence-id-261191

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  47. eIF2a 3

    Plasmid
    #21809
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S52D
    Promoter
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21809-plasmid-map-sequence-id-358561

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  49. pUK21

    Plasmid
    #49788
    Purpose
    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)
    Depositor
    Joachim Messing
    Article
    Vieira et al Gene. 1991 Apr;100:189-94.
    Type
    Empty backbone
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lacZ
    Available since
    July 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49788-plasmid-map-sequence-id-160334

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Showing: 3251 - 3300 of 19980 results