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173,387 results

Showing: 3301 - 3350 of 173387 results

  10. pMVP-SEC24C

    Plasmid
    #231559
    Purpose
    pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    silent PAM mutations (G67 (GGG>GGA) and A115 (…
    Promoter
    CMV
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231559-plasmid-map-sequence-id-459740

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  11. pJBL7048

    Plasmid
    #167218
    Purpose
    cell-free nitrate sensing reporter
    Depositor
    Julius Lucks
    Insert
    pNar-sfGFP
    Use
    Synthetic Biology
    Mutation
    ydfJ115 promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167218-plasmid-map-sequence-id-331012

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  13. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Expression
    Bacterial
    Promoter
    N/A
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190566-plasmid-map-sequence-id-393237

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  14. pD649-HAsp-LILRB5-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156860
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LILRB5 (LILRB5 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156860-plasmid-map-sequence-id-302829

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  17. pTSHZ2.1.0-gDNA

    Plasmid
    #113778
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TSHZ2
    Depositor
    Kevin White
    Insert
    TSHZ2 (TSHZ2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113778-plasmid-map-sequence-id-230569

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  21. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  26. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74224-plasmid-map-sequence-id-238509

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  36. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  42. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV2)

    Viral Prep
    #100836-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  43. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral Prep
    #137141-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Flp-dependent)
    Available Since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  45. pAAV-CAG-iSeroSnFR-Nlgn (AAV9)

    Viral Prep
    #128485-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31
    Promoter
    CAG
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  47. pAAV-hsyn-GRAB_5-HT1.0 (AAV9)

    Viral Prep
    #140552-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_5-HT1.0 (#140552). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_5-HT1.0 plasmid DNA. Synapsin-driven expression of GRAB_5-HT1.0 sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s
    Promoter
    Syn
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  48. pDV044

    Plasmid
    #191824
    Purpose
    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR
    Depositor
    Jim Roberts
    Article
    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi
    Type
    Empty backbone
    Use
    Unspecified
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191824-plasmid-map-sequence-id-375877

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  50. pAAV_hSynapsin_psychLight2 (AAV9)

    Viral Prep
    #163909-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV_hSynapsin_psychLight2 (#163909). In addition to the viral particles, you will also receive purified pAAV_hSynapsin_psychLight2 plasmid DNA. Synapsin-driven expression of the genetically encoded fluorescent sensor psychLight2 to detect behaviorally relevant serotonin release. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003
    Promoter
    Syn
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 3301 - 3350 of 173387 results