165,839 results
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Plasmid#64245Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable SinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only
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pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
gag-COM-pol
Plasmid#211373PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-COM-pol
UseTagsExpressionMammalianMutationPromoterAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS2
Plasmid#81037PurposeHO endonuclease expressionDepositorInsertHYGMX
UseTagsExpressionYeastMutationPromoterTEFAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
E. coli K-12 MG1655 RARE
Bacterial Strain#61440PurposeThis E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydesDepositorBacterial ResistanceNoneAvailable SinceFeb. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro
Plasmid#68469PurposeRetroviral plasmid with multiple cloning site and puromycin resistanceDepositorInsertPuromycin Resistance
UseRetroviralTagsExpressionMammalianMutationPromoterpGKAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMT3_SRSF1
Plasmid#201056PurposeExpress sumo-tagged human SRSF1DepositorAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-scCAK1
Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
UseTagsPolyhistidine-TEVExpressionInsectMutationPromoterAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pcDNA3
Plasmid#18956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-Lamin-A
Plasmid#206027PurposeExpresses GFP-tagged Lamin A in mammalian cellsDepositorAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX_lenti_osTir1_9Myc_P2A_Bsd
Plasmid#139746Purposelentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection markerDepositorInsertrice E3 ligase Tir1 (osTir1)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)
Viral Prep#50474-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA3mt
Plasmid#140464PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertR-CEPIA3mt
UseTagsMycExpressionMammalianMutationPromoterAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-nlsABACUS2-400n
Plasmid#203726PurposeGateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acidDepositorInsertnlsABACUS2-400n
UseSynthetic BiologyTagsMycExpressionMutationPromoterAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTMB352_ZFcharm_Kv1_split-inteins
Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-tmpknot-epegRNA-puro backbone
Plasmid#214089PurposeLentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-Cofilin
Plasmid#186747PurposeExpresses wildtype GFP-tagged human cofilin in mammalian cellsDepositorAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307 Luciferase
Plasmid#117734PurposeOpen reading frame vector encoding LuciferaseDepositorInsertfirefly luciferase
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
UseTagsbdSUMOExpressionBacterialMutationPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1-F-tractin-mCherry
Plasmid#155218PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cultivarium POSSUM Toolkit
Plasmid Kit#1000000234PurposeThe POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.DepositorAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-SF2
Plasmid#99021PurposeMammalian expression of SF2DepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVE-eGFP
Plasmid#52581PurposeLentiviral transfer vector driving expression of eGFP in mammalian cellsDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GreenGate 2.0 Toolkit
Plasmid Kit#1000000241PurposeThe GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.DepositorAvailable SinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_TetRVP64
Plasmid#79126PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG1
Plasmid#80795PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotypeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40
Plasmid#100848PurposeAAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NES
Plasmid#168301PurposeChemogenetic fluorescent H2O2 reporter targeted to the cytoplasmDepositorInsertHyPer7.2DAAO-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
tFucci(SA)5
Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorInserttFucci(SA)5.
UseTagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-mCherry-Frame +1
Plasmid#66940PurposeCRISPaint frame selector +1DepositorInsertgRNA frame +1
UseTagsExpressionMutationPromoterAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV8 trial size)
Viral Prep#37825-AAV8.TPurposeReady-to-use AAV8 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-IRES-EGFP
Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-EGFP-KASH
Plasmid#154374PurposeVector for Flp-dependent expression of EGFP tagged KASH protein domainDepositorInsertEGFP-KASH
UseAAVTagsExpressionMammalianMutationPromoterAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSmartBAC-S
Plasmid#107268PurposeBroad host range bacterial artificial chromosome vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)
Viral Prep#75470-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.Luc.IRES.EGFP.SV40
Plasmid#105533PurposeAAV expression of Luciferase and EGFP from CMV promoterDepositorInsertLuciferase
UseAAV and LuciferaseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-LAMP1-HaloTag
Plasmid#164209PurposeExpresses HaloTag tagged LAMP1 under CMV promoterDepositorAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.synP.DIO.EGFP.WPRE.hGH (AAV9)
Viral Prep#100043-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.synP.DIO.EGFP.WPRE.hGH (#100043). In addition to the viral particles, you will also receive purified pAAV.synP.DIO.EGFP.WPRE.hGH plasmid DNA. Synapsin-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-His6_pET21a
Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
1227 pcDNA3 Myr HA Akt2
Plasmid#9016DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2WT
Plasmid#101221PurposeBlue-light dependent optogenetic protein Cry2DepositorInsertCry2PHR
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterSFFVAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry (AAV2)
Viral Prep#50474-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7f-5p
Plasmid#103156PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7f-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only