Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

165,839 results

Showing: 3551 - 3600 of 165839 results

  1. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

    Enlarge

  3. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

  4. WT-MEK1-V5

    Plasmid
    #217297
    Purpose
    Lentivirus vector for expression of MEK1
    Depositor
    Channing Der
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217297-plasmid-map-sequence-id-431009

    Enlarge

  9. Dual-sgRNA_hU6-mU6

    Plasmid
    #154194
    Purpose
    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154194-plasmid-map-sequence-id-308648

    Enlarge

  10. pDUAL CD81 (GFP)

    Plasmid
    #86980
    Purpose
    Lentiviral expression construct encoding CD81 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    CD81 (CD81 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV/hPGK
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86980-plasmid-map-sequence-id-173290

    Enlarge

  15. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

    Enlarge

  18. pAAV-hSyn-mScarlet (AAV1)

    Viral Prep
    #131001-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie
    Promoter
    Syn
    Tags
    mScarlet
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-EF1a-fDIO-Cre (AAV9)

    Viral Prep
    #121675-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available Since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  21. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

    Viral Prep
    #50942-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#50942). In addition to the viral particles, you will also receive purified pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Mark Huebener, Tobias Rose, Tobias Bonhoeffer
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Promoter
    Syn
    Tags
    mRuby2
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  33. pAAV-Ef1a-Con/Foff 2.0-EYFP (AAV8)

    Viral Prep
    #137162-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-EYFP (#137162). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-EYFP plasmid DNA. EF1a-driven expression of EYFP in the presence of Cre (inhibited in the presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only

  40. FSW_jGCaMP8f

    Plasmid
    #197034
    Purpose
    Express GCaMP8f from hSyn1 promoter
    Depositor
    Ronald Hart
    Insert
    GCaMP8f
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197034-plasmid-map-sequence-id-392710

    Enlarge

  42. YIp204-PADH1-atTIR1-9myc

    Plasmid
    #99532
    Purpose
    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    TIR1 (TIR1 Mustard Weed)
    Use
    Tags
    9xMyc
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99532-plasmid-map-sequence-id-192011

    Enlarge

  44. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

    Enlarge

  49. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV Retrograde)

    Viral Prep
    #105558-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    None
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 3551 - 3600 of 165839 results