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Showing: 4351 - 4400 of 159781 results

  1. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  6. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

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  8. pAAV-hSyn-GRAB_CRF1.0

    Plasmid
    #208658
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    208658-plasmid-map-sequence-id-411118

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  9. pAAV_BiPVe4_ChR2_mCherry

    Plasmid
    #213937
    Purpose
    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry
    Depositor
    Gordon Fishell
    Article
    Furlanis et al bioRxiv 2024.07.17.603924
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin 2-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213937-plasmid-map-sequence-id-421852

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  18. pAAV-hSyn-hChR2(H134R)-mCherry (AAV9)

    Viral Prep
    #26976-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  22. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid
    #136348
    Purpose
    Lentiviral inducible expression (TetOn) of human BIRC5
    Depositor
    Guillermo de Cárcer
    Insert
    BIRC5 (BIRC5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136348-plasmid-map-sequence-id-270811

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  24. pAC-ZEAXipi

    Plasmid
    #53287
    Purpose
    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, idi, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53287-plasmid-map-sequence-id-82991

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  26. TFORF2821

    Plasmid
    #143196
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IKZF5 (IKZF5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143196-plasmid-map-sequence-id-391500

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  35. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  37. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. TFORF0144

    Plasmid
    #141536
    Purpose
    Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PHOX2A (PHOX2A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141536-plasmid-map-sequence-id-276744

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  40. Hs.HRAS

    Plasmid
    #83181
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83181-plasmid-map-sequence-id-158144

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  44. pEGFP-Snapin

    Plasmid
    #118742
    Purpose
    Expresses EGFP tagged Snapin
    Depositor
    Beverly Koller
    Insert
    Snapin cDNA (Snapin Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118742-plasmid-map-sequence-id-229079

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  45. pGBW-m4133747

    Plasmid
    #152494
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152494-plasmid-map-sequence-id-300234

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  46. AAV-CAG-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #179254-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CAG
    Tags
    None
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only

  49. pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (AAV8)

    Viral Prep
    #130991-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE (#130991). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE plasmid DNA. CamKIIa-driven, soma-targeted ChRmine-mScarlet expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie
    Promoter
    CaMKII
    Tags
    mScarlet
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 4351 - 4400 of 159781 results