32,069 results
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Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
lox2272 Neo* lox2272
Plasmid#168769Purposelox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
Tags6xHis and MBPExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLS7
Plasmid#32056PurposeVector optimized for constitutive gene expression in Burkholderia genus and related bacteriaDepositorTypeEmpty backboneExpressionBacterialPromoterPs7 promoter, originated from Paraburkholderia xe…Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA028
Plasmid#200419PurposethrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloningDepositorTypeEmpty backboneTagsPAmCherry1ExpressionBacterialAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + filler DNA reporter plasmid
Plasmid#156454PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsNeoR/KanR
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET42b-ABE7.10
Plasmid#120398PurposeExpresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertABE7.10
TagsHisExpressionBacterialMutationMammalian codon-optimizedPromoterT7Available SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-truncated
Plasmid#198286PurposeContains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCsDepositorInsertPVCpnf_pvc13-truncated
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
SB_concave
Plasmid#110102PurposepBXPHM3 with scaffold Sybody of the concave libraryDepositorInsertscaffold Sybody of the concave library
TagsHisExpressionBacterialPromoterPBADAvailable SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH5
Plasmid#199771PurposeExpresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)DepositorInsertcMSPdH5
TagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike
Plasmid#173921PurposeExpresses the original SARS-Cov-2 Spike Protein under a CMV PromoterDepositorInsertSARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
Tagsno tags, native proteinExpressionBacterial and MammalianPromoterstandard CMVAvailable SinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEVA3b67Rb
Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
ExpressionBacterial and YeastAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBDC
Plasmid#135188PurposeIntegration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.DepositorInsertCmR
UseSynthetic BiologyExpressionBacterialMutationNonePromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXT128_TwinStrep-SUMO-ShCas12k
Plasmid#135524PurposeProtein expression construct for ShCast12kDepositorInsertShCas12k
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCL149
Plasmid#134457PurposeCmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.DepositorInsertPara-popZ-rbs-H3H4-msfGFPN-CTM.
ExpressionBacterialPromoterParaAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKW106alp7A
Plasmid#69360PurposeStabilized lacZ expression vector containing alp7A and hok/sokDepositorInsertslacZ
alp7A
hok
placIq-lacI
ExpressionBacterialPromoterpTac and placIqAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-HRas-1-167-WT
Plasmid#245306PurposeExpresses G-domain of human HRas (residues 1-167 wild type) in E. coliDepositorInsertHRas (HRAS Human)
Tags6HisExpressionBacterialMutationdeleted amino acids 168-189PromoterT7lacAvailable SinceOct. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23B
Plasmid#201457PurposeExpresses human RAD23B with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T7-6h-GST-α-Synuclein
Plasmid#225224PurposeAlpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.DepositorInsertsGST
α-Synuclein
Tags6xHis Tag and TEV Cleavage SiteExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
TagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSZBAD1PG
Plasmid#11964DepositorInsertSspDnaEC
TagsGB1ExpressionBacterialAvailable SinceSept. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_13
Plasmid#60584Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_13
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2A
Plasmid#15780DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF3
Plasmid#70088PurposeEncode YTHDF3 isoform a with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11b-SpyCatcher-HA2
Plasmid#165991PurposeSpyCatcher-fused influenza HA ministem with removable HisTagDepositorInsertSpyCatcher-HA2
TagsFoldon, HisTag, and SpyCatcherExpressionBacterialPromoterT7Available SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFNR5
Plasmid#149483PurposeBroad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
VN1263 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_WeaKRBS_TIR51_KanR
Plasmid#235115PurposeQuantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.DepositorInsertsUseSynthetic Biology; Quantitative bacterial two-hyb…TagscI_E34P-rpoAExpressionBacterialPromoterLtetO and lpp+lacUV5Available SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU, aa 52-209)
Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINM
Plasmid#26044DepositorInsertHis-MBP-3C-lacZ
TagsMAHHHHHHSSG_MBP_LEVLFQGPExpressionBacterial, Insect, and Mamm…Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
ExpressionBacterial and MammalianPromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-Z1-PETase
Plasmid#218703PurposeEncodes codon optimized Z1-PETase enzyme for bacterial expressionDepositorInsertZ1-PETase
Tags10xHisExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean
Plasmid#103968PurposePlasmid encoding mCeruleanDepositorInsertmCerulean
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterProCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only