32,156 results
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Plasmid#51557PurposeE. coli fis ORF cloned into pBAD24 expression vectorDepositorInsertFIS (fis E. coli)
ExpressionBacterialAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
Tags6XHIS tagExpressionBacterialPromoterT7Available SinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSum-LacZ-gfp
Plasmid#109285PurposeExpresses gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationGFP modified for expression in prokaryotes; inclu…PromoterLacZalphaAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-T17N
Plasmid#22029PurposeExpression of photoactivatable Rac1 (dominant-negative mutant).DepositorInsertPA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545-dCas9Str
Plasmid#153516PurposeExpresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backboneDepositorInsertdCas9Str
ExpressionBacterialPromoternisAAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
TagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
TagsHis6x tagExpressionBacterialPromoterT7Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C
Plasmid#159540PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12CDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12CAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hfYFP
Plasmid#186522PurposeHyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertHyperfolder YFP
Tags6xHisExpressionBacterialMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
TagsNT* tag derived from spider silk proteinsExpressionBacterialPromoterT7Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-CatC-HA-CfaNm
Plasmid#241424PurposeProtein Transposon Cassette Containing CatC and CfaCNmDepositorInsertHis5-Sumo-CatC-HA-CfaNm
TagsHis5ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-15b_T. maritima His-tRNase Z
Plasmid#242436PurposeExpresses T. maritima tRNase Z in E. coli.DepositorInserttRNase Z
Tags6x HisExpressionBacterialPromoterT7Available SinceSept. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVRb19_up1315
Plasmid#49713DepositorInsertsfgfp
UseSynthetic BiologyExpressionBacterialPromoterPecf19_up1315Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsR_TS-E10
Plasmid#137944PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expressionDepositorInsertsmRFP1
mWasabi
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101 and pTlpA-C7Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-mCherry
Plasmid#136615PurposeDox-inducible lentivral vector expressing mCherry red fluorescent proteinDepositorInsertmCherry
UseLentiviralExpressionBacterialAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC_MS2_DNA_VLP
Plasmid#179156PurposeExpression of maturation protein and his-tagged coat protein dimer from MS2 phageDepositorInsertsMaturation Protein
Coat Protein Dimer
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOSIP-KT (KanR, P21)
Plasmid#45987DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
NSUN5
Plasmid#25491PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAUC40
Plasmid#71298PurposeSuicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-StefinA
Plasmid#182405PurposeBacterial expression of Vesicle Nucleating peptide15-stefinA fusionDepositorInsertVNp15-stefin (CSTA Human)
TagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD-KPS12-Pman
Plasmid#133832PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKEW336-MBP-TEV-MbCas12a-33362
Plasmid#115670PurposeExpresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purificationDepositorInsertMbCas12a
UseCRISPRTagsMBP-TEVExpressionBacterialPromoterT7 RNAPAvailable SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pttq18
Plasmid#69122Purposeregulated expression of genes in Escherichia coliDepositorTypeEmpty backboneExpressionBacterialPromotertacAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-pHAT
Plasmid#83928PurposehsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorAvailable SinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
plRL61
Plasmid#163633PurposeOptimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
TagsN-His6-GST-3CExpressionBacterialPromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only