Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,239 results

Showing: 5201 - 5250 of 76239 results

  1. TFORF1479

    Plasmid
    #143504
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143504-plasmid-map-sequence-id-400403

    Enlarge

  7. pCMV3flag8HOIL-1L

    Plasmid
    #50016
    Purpose
    Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.
    Depositor
    Martin Dorf
    Article
    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2
    Insert
    RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
    Use
    Tags
    3 x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    50016-plasmid-map-sequence-id-169718

    Enlarge

  8. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128059-plasmid-map-sequence-id-256675

    Enlarge

  10. TFORF3007

    Plasmid
    #144483
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMAD1 (SMAD1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144483-plasmid-map-sequence-id-407358

    Enlarge

  21. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179430
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-HS4-mch (SH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179430-plasmid-map-sequence-id-355528

    Enlarge

  24. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

    Enlarge

  26. pLKO.1 mCherry

    Plasmid
    #128073
    Purpose
    puro exchanged to mCherry
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    no
    Promoter
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128073-plasmid-map-sequence-id-255273

    Enlarge

  34. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid
    #221357
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221357-plasmid-map-sequence-id-436816

    Enlarge

  35. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid
    #221358
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221358-plasmid-map-sequence-id-436819

    Enlarge

  36. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

    Enlarge

  39. pHRIG-Akt1

    Plasmid
    #53583
    Purpose
    lentiviral expression of constitutively active human Akt1 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    cAkt1 (AKT1 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Oct. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53583-plasmid-map-sequence-id-448192

    Enlarge

Showing: 5201 - 5250 of 76239 results