76,036 results

1. pRC54-DHX37-GFP

   Plasmid

   #192149

   Purpose

   express DHX37-GFP

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Insert

   DHX37-GFP (Dhx37 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DPYSL2-pLJC2

   Plasmid

   #189866

   Purpose

   Expresses human DPYSL2 (CRMP2) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

   Insert

   DPYSL2 (DPYSL2 Human)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

   Plasmid

   #192636

   Purpose

   Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HTR1B-Tango

   Plasmid

   #66405

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HTR1B (HTR1B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Raptor_2 shRNA

   Plasmid

   #1858

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTagRFP-T-VASP-5

   Plasmid

   #58027

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   VASP
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCMV-Neo-Bam p53 wt

   Plasmid

   #16434

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NES-EGFP-cPHx3

   Plasmid

   #116855

   Purpose

   PI(3,4)P2 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amino acids 169-329 (tandem trimer)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

   Plasmid

   #100833

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB-cT3G-cERP2-HDAC2

    Plasmid

    #222553

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HDAC2 (HDAC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-IRES_Tomato

    Plasmid

    #107229

    Purpose

    Tomato expressing plasmid

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid

    #127090

    Purpose

    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Has Service

    AAV PHP.eB

    Insert

    Channelrhodopsin-2
    

    Use

    AAV

    Tags

    eYFP (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Humanized ChR2 gene with histidine 134 changed to…

    Promoter

    CAG

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX302

    Plasmid

    #25896

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti_rtTA-P2A-BFP_HygroR

    Plasmid

    #186738

    Purpose

    rtTA-P2A-BFP expression construct cloned into lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRES proHB-EGF WT

    Plasmid

    #11602

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

17. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-CAG-hCas3

    Plasmid

    #134920

    Purpose

    Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas3 with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid

    #198451

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid

    #198914

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. hnRNPA1_D262V-FLAG-IRES-eGFP

    Plasmid

    #234620

    Purpose

    Mammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequence

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1 D262V (HNRNPA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Famillial ALS mutation D262V, C-terminal FLAG tag…

    Promoter

    CMV

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

    Plasmid

    #228721

    Purpose

    Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-FIP200(1-640)-MBP

    Plasmid

    #203543

    Purpose

    FIP200 NTD-GFP mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    MBP-10His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pD649-HAsp-PTPRQ(Trunc1)-COMP5AP-AviTag-9xHis

    Plasmid

    #157614

    Purpose

    Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PTPRQ (PTPRQ Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-MBP-ATG9

    Plasmid

    #188076

    Purpose

    Recombinant overexpression of human ATG9 in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFX-iRFP-Rab7

    Plasmid

    #174459

    Purpose

    Express iRFP with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with late endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Use

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-PE-Plus

    Plasmid

    #214079

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMV-G-GECO1.2

    Plasmid

    #32446

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLDPuro-hsnSR100N

    Plasmid

    #35172

    Depositor

    Benjamin Blencowe

    Article

    Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

    Insert

    SRRM4 (SRRM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 1b34

    Plasmid

    #177692

    Purpose

    HCV E1E2 expression plasmid

    Depositor

    Justin Bailey

    Article

    Salas et al Gastroenterology. 2022 Feb;162(2):562-57

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENN.AAV.CAG.tdTomato.WPRE.SV40

    Plasmid

    #105554

    Purpose

    AAV expression of tdTomato from CAG promoter

    Depositor

    James M. Wilson

    Insert

    tdtomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEmerald-N1

    Plasmid

    #53976

    Purpose

    Localization: N1 Cloning Vector, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA4/Full length FVIII

    Plasmid

    #41036

    Depositor

    Robert Peters

    Article

    Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

    Insert

    FVIII (F8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid

    #107790

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMVTNT-T7-KPNA4

    Plasmid

    #26680

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 4 (KPNA4 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge