76,416 results

1. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

   Plasmid

   #154850

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dCas9-GCN5 FL

   Plasmid

   #179552

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mEmerald-ARP2-C-14

   Plasmid

   #53992

   Purpose

   Localization: Actin Related Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   ARP2 (ACTR2 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDONR221_SLC1A4

   Plasmid

   #131962

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A4 (SLC1A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pIC291

   Plasmid

   #51403

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska1 IMAGE 3909951 (SKA1 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL-p27UTR

   Plasmid

   #20881

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p27 3'UTR (Cdkn1b Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pBabe-puro Ras V12

   Plasmid

   #1768

   Depositor

   Bob Weinberg

   Insert

   ras V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V, constitutively active

   Promoter

   Available since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mNeonGreen-UtrCH

   Plasmid

   #98879

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   UtrCH
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCEP4-myc-HLA-B7

   Plasmid

   #135509

   Purpose

   Mammalian expression of myc-tagged HLA-B*07:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*07:02 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAT077

    Plasmid

    #180511

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

    Plasmid

    #187574

    Purpose

    Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    RNPS1 (RNPS1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HTR1F-Tango

    Plasmid

    #66408

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1F (HTR1F Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. aCKAR

    Plasmid

    #124427

    Purpose

    FRET-based reporter for monitoring aPKC activity in cells

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

    Insert

    aCKAR
    

    Use

    Tags

    mCFP and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-HDAC6.ΔBuz-FLAG

    Plasmid

    #30484

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal Ubiquitin binding doma…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-C-dTAG-Blast

    Plasmid

    #207716

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3-sACE2(WT)-Fc(IgG1)

    Plasmid

    #145163

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV_Stargazin-mTurquoise2-iLID

    Plasmid

    #161001

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Stargazin-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA)

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFSp-GFP-YAP

    Plasmid

    #174168

    Purpose

    Lentiviral vector to constitutively express YAP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. Flag-HA-USP16

    Plasmid

    #22595

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP16 (USP16 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-Fzd4 subtype NGS Wnt

    Plasmid

    #159625

    Purpose

    Express Fzd4 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd4 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1/Puro-CAG-JEDI-1P

    Plasmid

    #202607

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV p16 INK4A

    Plasmid

    #10916

    Depositor

    Bob Weinberg

    Article

    Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

    Insert

    p16 INK4 (CDKN2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ptdTomato-SMCR8

    Plasmid

    #83438

    Purpose

    Expresses SMCR8-tdTomato in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    Human SMCR8 (SMCR8 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMSCVII

    Plasmid

    #162750

    Purpose

    MSCV backbone

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC1-HyPerRed-mito

    Plasmid

    #60247

    Purpose

    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HyPerRed-mito
    

    Use

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GPR78-Tango

    Plasmid

    #66374

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR78 (GPR78 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pRK5-HA-Sestrin2

    Plasmid

    #72593

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

30. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDIRE

    Plasmid

    #26745

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    iCre expression cassette
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFH2.9_MMLV_Gag-P2A-eGFP

    Plasmid

    #205524

    Purpose

    Express MMLV Gag-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

    Plasmid

    #221361

    Purpose

    Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC; to be used with Anti-ProC [IPI-HPC-4] light chain (Plasmid 221362) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa

    Plasmid

    #221362

    Purpose

    Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa; to be used with Anti-ProC [IPI-HPC-4] heavy chain (Plasmid 221361) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid

    #155043

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG2-EGFP-C-IFT144

    Plasmid

    #218740

    Purpose

    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT144 (WDR19 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. phNFM

    Plasmid

    #132597

    Purpose

    Expresses human NFM

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFM (NEFM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ACTN2-pEGFP

    Plasmid

    #52669

    Purpose

    Overexpression of GFP-tagged α-actinin-2

    Depositor

    Johannes Hell

    Article

    Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

    Insert

    actinin 2 (ACTN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. M_ST1n_VPR

    Plasmid

    #63799

    Purpose

    ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    ST1-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEST47-CYFIP1.GFP

    Plasmid

    #109139

    Purpose

    GFP-tagged CYFIP1 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. mNeptune2-C1

    Plasmid

    #54836

    Purpose

    Localization: C1 Cloning Vector, Excitation: 600, Emission: 650

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mNeptune2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pcDNA-Wnt6

    Plasmid

    #35913

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt6 (WNT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent Mutation G (567) --> A

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFPN1-human Dynamin 1aa K44A

    Plasmid

    #22197

    Depositor

    Pietro De Camilli

    Article

    Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

    Insert

    human Dynamin 1aa K44A (DNM1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. KU70-eGFP

    Plasmid

    #213517

    Purpose

    Expresses human KU70-eGFP in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    human KU70-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAV-TRE-GCaMP6s-WPRE-sv40

    Plasmid

    #184414

    Purpose

    AAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNTI825

    Plasmid

    #216633

    Purpose

    Cal-ID (V5) for AAV production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PH-Akt(R25C)-GFP

    Plasmid

    #51466

    Purpose

    Non-PIP3 binding control for PH-Btk-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

48. pDY1314_RADARS_Luciferase

    Plasmid

    #193191

    Purpose

    Express Gaussia Luciferase conditional upon RNA transcript

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    RADARS Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCAG-Sema4D-Flag

    Plasmid

    #190646

    Purpose

    Expresses Mouse Sema4D with C Terminal Flag Tag

    Depositor

    Victor Tarabykin, Andrew Newman

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCAG-sp-myc-mSema4D

    Plasmid

    #190645

    Purpose

    Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

    Depositor

    Victor Tarabykin, Andrew Newman

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge