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pNP2322 [Cloning backbone, mCherry Inversion Activity Reporter]

Plasmid

#247250

Purpose

Cloning backbone for measuring bridge recombinase mediated inversion

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-gfaABC1D-PinkyCaMP

Plasmid

#232859

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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LT3REVIR

Plasmid

#111176

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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tdTomato-N1

Plasmid

#54642

Purpose

Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Promoter

CMV/R/U5

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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FU- dio PSD95-mCherry- W

Plasmid

#73919

Purpose

Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

PSD-95-mCherry

Use

Lentiviral

Tags

mCherry and myc

Expression

Mammalian

Promoter

Ubi-C

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVtight-UPRT-T2A-RFP-IRES-CD

Plasmid

#126677

Purpose

Doxycycline inducible expression of UPRT and CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP2

Plasmid

#59037

Purpose

for baculovirus expression of CAMSAP2 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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10279-M30-663

Plasmid

#225663

Purpose

Lentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCherry

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-N LMP1

Plasmid

#37962

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

LMP1 (LMP-1 H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only

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GFP::L4440

Plasmid

#11335

Depositor

Guy Caldwell

Insert

GFP

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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CRY2olig-FUS

Plasmid

#218921

Purpose

Expression of CRY2olig-mCherry fused to FUS

Depositor

Tatyana Shelkovnikova

Insert

CRY2olig-mCherry tagged FUS

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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OPRM1-Tango

Plasmid

#66464

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

OPRM1 (OPRM1 Human)

Tags

FLAG and tTA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pICH41744

Plasmid

#48017

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-hSyn-JEDI-2P-WPRE

Plasmid

#179464

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL CD86 HLA-A201

Plasmid

#183248

Purpose

Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Inserts

CD86, 41BBL
HLA-A2 (HLA-A Human)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2

Plasmid

#17999

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_JN.1

Plasmid

#218678

Purpose

expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

Depositor

David Nemazee

Insert

Spike (JN.1)

Expression

Mammalian

Mutation

L455S on the basis of BA.2.86

Promoter

CMV

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7

Plasmid

#62276

Purpose

mammalian expression of human Alpha7 (CHRNA7)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRNA7 (CHRNA7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-2xFLAG-SREBP-1a

Plasmid

#26801

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1a (SREBF1 Human)

Tags

2xFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCold CL7-Cas12a

Plasmid

#124891

Purpose

obtaining full Cas12a RNP

Depositor

Yi Liu

Article

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas12a

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA GFP10-ZIP-GFP11

Plasmid

#182243

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP-11

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/CIP2A(1-905) WT V5 His

Plasmid

#119287

Purpose

CIP2A WT V5 overexpression

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Tags

V5 His

Expression

Mammalian

Promoter

T7

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

Plasmid

#216478

Purpose

lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
tagBFP

Use

Cre/Lox and Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE TRE dCas9-VP64

Plasmid

#50916

Purpose

Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A nuclease-deficient

Promoter

TRE

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CaMKAR

Plasmid

#205315

Purpose

Fluorescent Reporter for CaMKII Activity

Depositor

Mark Anderson, Elizabeth Luczak

Article

Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

Insert

CaMKAR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-5X-1-Full-Length-MePCE

Plasmid

#113539

Purpose

Produces an N-terminal GST-tagged full length MePCE protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_ALFA-tag-SNAP

Plasmid

#136628

Purpose

Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA_Cloning Vector BbsI

Plasmid

#128433

Purpose

Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

Depositor

Izuho Hatada

Type

Empty backbone

Expression

Mammalian

Promoter

U6 promoter

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

Plasmid

#216479

Purpose

lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

wtYAP (YAP1) (YAP1 Human)
tagBFP

Use

Cre/Lox and Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-GRAB_ATP1.0

Plasmid

#167577

Purpose

Express the ATP sensor GRAB_ATP1.0 in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Article

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
ankG-mCherry

Plasmid

#42566

Depositor

Benedicte Dargent

Article

Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

ankyrin G (Ank3 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
EC67907

Plasmid

#211794

Purpose

Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas9, GR-GRF-GIF

Expression

Plant

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti sfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164478

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
nlsSalicS1

Plasmid

#232190

Purpose

Novel FRET-based biosensor for salicylic acid

Depositor

Alexander Jones

Article

Tang et al Science. 2025 Oct 9;390(6769):188-194. d

Insert

nlsSalicS1

Tags

His-tag

Expression

Plant

Promoter

p16

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41722

Plasmid

#48016

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAC-69

Plasmid

#31241

Purpose

Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish

Depositor

Seok-Yong Choi

Article

Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

Insert

mitochondrial localization enhanced green fluorescent protein

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
YAP1-V5 in pLX304

Plasmid

#42555

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTEF2

Plasmid

#43971

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-(-2)AP-Ub

Plasmid

#232582

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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