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Showing: 4951 - 5000 of 157990 results

  2. TFORF0889

    Plasmid
    #143735
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF384 (ZNF384 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143735-plasmid-map-sequence-id-415860

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  5. pCDH-CMV

    Plasmid
    #72265
    Purpose
    Express gene of interest under CMV promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72265-plasmid-map-sequence-id-436455

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  6. PB-TO-NFIA.1-SOX9

    Plasmid
    #182310
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182310-plasmid-map-sequence-id-359522

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  11. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

    Plasmid
    #44361
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44361-plasmid-map-sequence-id-148598

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  24. pLEX305_3xFLAG_STAT1

    Plasmid
    #220323
    Purpose
    Expresses full length human STAT1 with an N-terminal 3xFLAG tag
    Depositor
    Jonathan Brown
    Insert
    STAT1 alpha (STAT1 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    PGK
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220323-plasmid-map-sequence-id-437225

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  25. pBBR-Cas9ΔyqhD-HA500

    Plasmid
    #220369
    Purpose
    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
    Depositor
    Yilan Liu
    Inserts
    SpCas9
    sgRNA for yqhD in P. putida KT2440
    Use
    CRISPR; Broad host range
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220369-plasmid-map-sequence-id-437258

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  26. pLenti.PGK.LifeAct-GFP.W

    Plasmid
    #51010
    Purpose
    Lentivector that expresses LifeAct-GFP from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-GFP
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51010-plasmid-map-sequence-id-291815

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  28. pAC-CANTHipi

    Plasmid
    #53301
    Purpose
    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtO
    Use
    Low copy number bacterial cloning vector
    Tags
    6His
    Expression
    Mutation
    Promoter
    Trc
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53301-plasmid-map-sequence-id-99820

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  33. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid
    #50478
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50478-plasmid-map-sequence-id-166302

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  34. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

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  39. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125518-plasmid-map-sequence-id-248252

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  44. Hs.NRAS

    Plasmid
    #83173
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83173-plasmid-map-sequence-id-158136

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  46. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid
    #61593
    Purpose
    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
    Depositor
    Feng Zhang
    Article
    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    HA, NLS, and OLLAS tag
    Expression
    Mammalian
    Mutation
    K175R and K736R (deUb mutations)
    Promoter
    Available since
    Feb. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61593-plasmid-map-sequence-id-470051

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  49. pIRES-H3.3-WT-FLAG-BFP

    Plasmid
    #206139
    Purpose
    Expression of a FLAG tagged human histone H3.3 gene with BFP
    Depositor
    Maria Castro
    Insert
    H3.3 (H3-3A Human)
    Use
    Tags
    FLAG-eBFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    206139-plasmid-map-sequence-id-405576

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Showing: 4951 - 5000 of 157990 results