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171,784 results

Showing: 5001 - 5050 of 171784 results

  1. pMAL-AGR2

    Plasmid
    #18097
    Depositor
    Ronny Drapkin
    Insert
    AGR2 (AGR2 Human)
    Tags
    MBP
    Expression
    Bacterial
    Available Since
    June 11, 2008
    Availability
    Academic Institutions and Nonprofits only

  8. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62201-plasmid-map-sequence-id-228092

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  9. pShuttle-CMV-GFP

    Plasmid
    #128065
    Purpose
    Adenoviral shuttle vector with CMV promoter-EGFP
    Depositor
    Oskar Laur
    Insert
    CMV promoter-EGFP
    Use
    Adenoviral
    Mutation
    no
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128065-plasmid-map-sequence-id-255181

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  10. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    human PGK
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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  12. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  20. pUC19-P70a-3WJdB-T

    Plasmid
    #87311
    Purpose
    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Three-way Junction dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    P70a
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87311-plasmid-map-sequence-id-174712

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  23. TFORF0335

    Plasmid
    #144358
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF91 (ZNF91 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144358-plasmid-map-sequence-id-352989

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  25. TFORF2941

    Plasmid
    #144417
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DLX5 (DLX5 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144417-plasmid-map-sequence-id-391584

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  26. pcpBpaRS

    Plasmid
    #196484
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    CMV (synthetase), Pol III tRNA promoter (tRNA)
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196484-plasmid-map-sequence-id-388864

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  32. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190034-plasmid-map-sequence-id-371641

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  36. pCB265

    Plasmid
    #69748
    Purpose
    Retroviral expression of HURP
    Depositor
    Iain Cheeseman
    Insert
    HURP (DLGAP5 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Dec. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. IDG_TMEM63C_OE_1

    Plasmid
    #161695
    Purpose
    Overexpresses TMEM63C
    Depositor
    Mike McManus
    Insert
    TMEM63C (TMEM63C Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    July 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    161695-plasmid-map-sequence-id-319730

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  44. BTV

    Plasmid
    #84771
    Purpose
    bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production
    Depositor
    David Erle
    Article
    Zhao et al Nat Biotechnol. 2014 Apr;32(4):387-91. d
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    84771-plasmid-map-sequence-id-162487

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  45. IDG_CACNG8_OE_1

    Plasmid
    #161698
    Purpose
    Overexpresses CACNG8
    Depositor
    Mike McManus
    Insert
    CACNG8 (CACNG8 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Dec. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161698-plasmid-map-sequence-id-319344

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  46. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  47. TFORF0196

    Plasmid
    #143204
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF844 (ZNF844 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143204-plasmid-map-sequence-id-396099

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Showing: 5001 - 5050 of 171784 results