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We narrowed to 9,391 results for: Coli

Showing: 4121 - 4160 of 9391 results

  1. pTU2-A-RFP (p15A origin)

    Plasmid
    #74090
    Purpose
    pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    R0010 Const. Promoter controlling RFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74090-plasmid-map-sequence-id-138311

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  2. pET22b_EcSSB_1

    Plasmid
    #78203
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1
    Insert
    ssb (G26C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G26C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78203-plasmid-map-sequence-id-151729

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  3. pCW17 - pAdapter

    Plasmid
    #174664
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    MS2 coat protein
    Use
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174664-plasmid-map-sequence-id-361957

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  4. pDP085

    Plasmid
    #198425
    Purpose
    pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120
    Depositor
    Markus Schmid
    Article
    You et al Nat Commun. 2017 May 17;8:15120. doi: 10
    Insert
    E. coli BirA
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    198425-plasmid-map-sequence-id-388239

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  6. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

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  8. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  9. pRB117.2

    Plasmid
    #181949
    Purpose
    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181949-plasmid-map-sequence-id-356570

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  14. pTU2-A-RFP (colE1 origin)

    Plasmid
    #74093
    Purpose
    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    R0010 Const. Promoter controlling RFP
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74093-plasmid-map-sequence-id-138340

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  16. pSCL.028

    Plasmid
    #184965
    Purpose
    Test effect of extending -Eco1 a1/a2 in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    Use
    Tags
    Expression
    Yeast
    Mutation
    human codon optimized RT, a1/a2 length extended t…
    Promoter
    Gal7
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184965-plasmid-map-sequence-id-376534

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  17. pTU2S-b (p15A origin)

    Plasmid
    #74089
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74089-plasmid-map-sequence-id-138299

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  20. pT2SCb

    Plasmid
    #59385
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-cat2
    Use
    Template
    Tags
    Expression
    Mutation
    The 3’-end of the chloramphenicol resistance gene…
    Promoter
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59385-plasmid-map-sequence-id-92126

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  21. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

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  23. pTS2356_Tier3(PB)-PuroR

    Plasmid
    #169655
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169655-plasmid-map-sequence-id-364560

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  25. pET28a-baPrs-hdNadV

    Plasmid
    #91950
    Purpose
    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi
    Depositor
    George Cătălin Marinescu
    Article
    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10
    Inserts
    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Leucine 135 to Isoleucine
    Promoter
    T7
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91950-plasmid-map-sequence-id-194125

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  29. prCAG-RBS"m1"-sfGFP

    Plasmid
    #191400
    Purpose
    Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)
    Depositor
    Ariel Lindner
    Article
    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.
    Insert
    47xCAG RBS"m1"-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191400-plasmid-map-sequence-id-382526

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  33. pSC201

    Plasmid
    #129387
    Purpose
    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.
    Depositor
    Silvia Cardona
    Article
    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do
    Insert
    oriR6K and oriT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    N/A
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    129387-plasmid-map-sequence-id-257366

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  36. pLaNGo11-1

    Plasmid
    #126053
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126053-plasmid-map-sequence-id-255676

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  40. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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Showing: 4121 - 4160 of 9391 results