20,112 results
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Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorInsertER localized P450
ExpressionBacterial and MammalianAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.PI.Lck-GFP.SV40
Plasmid#105598PurposeAAV expression of membrane bound EGFP from GfaABC1D promoterDepositorHas ServiceAAV5InsertLck-EGFP
UseAAVExpressionMammalianPromoterGfaABC1DAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2FLAG
Plasmid#16331DepositorInsertFlag tag
UseVertebrate expressionTagsFlagMutationFlag tag inserted into pCS2+ polylinkerAvailable SinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-HA-humanCMYC
Plasmid#74164PurposeMammalian expression of human cMycDepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p46Cpf1-OP2
Plasmid#98592PurposeProduces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationCodon optimized for E. coliPromoteraraBADAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of Pa01 recombinase from Ef1a promoterDepositorInsertPa01
ExpressionMammalianPromoterEf1aAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-Fc-His
Plasmid#72104PurposeExpresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-LC3
Plasmid#11546DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)
Plasmid#48285DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable SinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ezh2[SET]-dCas9
Plasmid#100087PurposeCatalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertEzh2[SET] (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[SET]-dCas9-NLSExpressionMammalianMutationEzh2[SET] includes aa 482-746PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionMutationDetailed in manuscriptPromoterT7 (inactivated)Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-EGFP-WPREpA
Plasmid#84469PurposepAAV-RAM-EGFPDepositorInsertEGFP
UseAAVPromoterTREAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
psfaMs-Tn5
Plasmid#183637PurposeExpresses anti-mouse nanobody fused with Tn5DepositorInsertanti mouse secondary nanobody fused to Tn5
Tagsanti-mouse nanobodyExpressionBacterialAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
ExpressionBacterialPromoterno promoterAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-IMP2-2
Plasmid#42175DepositorInsertInsulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
TagsGFPExpressionMammalianPromoterT7Available SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-3xHA
Plasmid#102932PurposeLentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)DepositorInsertLAMP1
UseLentiviralTagsRFP-HAPromoterubcAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_STA5A
Plasmid#167799PurposeExpresses STA5A in mammalian cellsDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BAF
Plasmid#172447PurposeExpression of nuclear protein BAF with an N-terminal mCherry tagDepositorInsertBAF
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-acc(3)IV
Plasmid#69862PurposepCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceNov. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N EBNALP
Plasmid#37959DepositorInsertEBNALP (EBNA-LP H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianPromoterPKGAvailable SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Venus (EDCPV)
Plasmid#90085PurposeThis plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianPromoterEFSAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
repressilator (pZS1-lTlrLLtCL)
Plasmid#26489DepositorInsertLacI, tetR
ExpressionBacterialAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJL-TRBO-G
Plasmid#80083PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.DepositorInsertGFP
ExpressionPlantPromoterCaMV35SAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-KFERQ-NE
Plasmid#102365PurposeLentiviral overexpression of PA-mCherry-KFERQ fusionDepositorInsertsPA-mCherry
KFERQ peptide
UseLentiviralTagsNEExpressionMammalianPromoterEF-1aAvailable SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-ERK2-L4A-MEK1_fusion
Plasmid#39197DepositorTagsMycExpressionMammalianMutationL4A = L33A, L37A, L40A, L42APromoterCMVAvailable SinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-YAP
Plasmid#174172PurposeLentiviral vector to constitutively express YAP under control of the human PGK promoter.DepositorAvailable SinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits