19,178 results

1. ymScarlet

   Plasmid

   #111917

   Purpose

   yeast optimized mScarlet

   Depositor

   Patrick Lajoie

   Article

   Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

   Insert

   ymScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmCherry-U6-empty

   Plasmid

   #140580

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti Lifeact-iRFP670 BlastR

   Plasmid

   #84385

   Purpose

   Lentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSET-mSA

   Plasmid

   #39860

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOlyA(E69A)

   Plasmid

   #132392

   Purpose

   Mutant version of OlyA that senses sphingomyelin without or with cholesterol

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A point mutant (E69A)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, E69A, C94S, S151C

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-EML4-ALK variant 1

   Plasmid

   #183828

   Purpose

   Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-13 and ALK exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFPC2-Gephyrin P1

   Plasmid

   #68815

   Purpose

   expression of fluorescent tagged Geph in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyri…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 Mfn2-HA

   Plasmid

   #139192

   Purpose

   Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DNA-PKcs PQR>ala

   Plasmid

   #83319

   Purpose

   human DNA-PKcs PQR>ala

   Depositor

   Katheryn Meek

   Article

   Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5 phosphorylation sites in the PQR cluster substi…

   Promoter

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET His6 MBP prescission LIC cloning vector (HMPKS)

    Plasmid

    #29721

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and Prescission cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

    Plasmid

    #106309

    Purpose

    The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
    

    Use

    Tags

    6xflag-3xHaloTag(MtoL)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28CLY1

    Plasmid

    #21760

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible one SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Human pIgR(SC)

    Plasmid

    #158216

    Purpose

    Express the ectdomain (SC) of human pIgR

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    PIGR (PIGR Human)
    

    Use

    Tags

    8×his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Fuw-dCas9-Dnmt3a-P2A-tagBFP

    Plasmid

    #84569

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGIP

    Plasmid

    #26777

    Purpose

    Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    EF1a-GFP-IRES-Puro (gfp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHairyRed

    Plasmid

    #69709

    Purpose

    Fluorescent reporter of Agrobacterium rhizogenes mediated transformed roots

    Depositor

    Peter Gresshoff, Brett Ferguson

    Article

    Lin et al Mol Plant. 2011 May;4(3):537-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-minCMV-mCherry

    Plasmid

    #27970

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nrf1-Myc pcDNA3.1(+)-C-Myc

    Plasmid

    #169125

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_hSynapsin_psychLight2

    Plasmid

    #163909

    Purpose

    To generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoter

    Depositor

    Lin Tian

    Article

    Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

    Has Service

    AAV9

    Insert

    psychLight2
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBabe-GFP-IKBalpha-mut (super repressor)

    Plasmid

    #15264

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLPC-NFLAG TRF1

    Plasmid

    #16058

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    aa2-439

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

23. pLNCX2-Scrib

    Plasmid

    #24600

    Depositor

    Senthil Muthuswamy

    Article

    Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

    Insert

    Scribble (SCRIB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGGS_L_EBOV

    Plasmid

    #103052

    Purpose

    Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    L gene of Ebola virus (L Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hTLR4

    Plasmid

    #13086

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (TLR4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

    Plasmid

    #175544

    Purpose

    Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag9-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRXIP TMEM41B-GFP

    Plasmid

    #117414

    Purpose

    Expresses TMEM41B tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-Neo-Bam p53 R249S

    Plasmid

    #16438

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R249S (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R249S

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.3_KLF4

    Plasmid

    #26815

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-KLF4-3'UTR (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Starts from second ATG

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHsp83-NLS-HA-2xMCP-2xTagRFP-T

    Plasmid

    #71242

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    NLS-2xMCP-2xTagRFP-T
    

    Use

    P element-mediated germline transformation

    Tags

    TagRFP-T

    Expression

    Insect

    Mutation

    Promoter

    Hsp83

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. hCas9_D10A

    Plasmid

    #41816

    Purpose

    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9_D10A
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A nickase

    Promoter

    CMV

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EGFR D770-N771 insNPG

    Plasmid

    #11016

    Purpose

    Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR D770-N771 insNPG (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D770-N771 insNPG

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

37. Flag-HA-USP46

    Plasmid

    #22584

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP46 (USP46 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNCA

    Plasmid

    #17363

    Depositor

    Stephen Goff

    Article

    Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

    Insert

    MMLV proviral DNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pSLIK-IDH2-R172K-FLAG

    Plasmid

    #66807

    Purpose

    Tet-inducible expression of mutant IDH2 in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH2 (IDH2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FLAG-Stx17

    Plasmid

    #45911

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Myc-Nix

    Plasmid

    #100795

    Purpose

    Mammalian expression of Nix (Bnip3L)

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    BCL2 interacting protein 3 like (BNIP3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Green Pegassos

    Plasmid

    #163114

    Purpose

    Expresses biosensors for pyruvate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Pegassos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pICE-EGFP-FLAG-PLIN3

    Plasmid

    #161918

    Purpose

    Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Perilipin-3 (PLIN3 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. SNAP-Sec61B

    Plasmid

    #141152

    Purpose

    Fluorescent labeling of ER membrane in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    L79M on Sec61B

    Promoter

    CMV

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGL3-Basic-8x-gRNA-eGFP

    Plasmid

    #60718

    Purpose

    eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-synapsin-HaloCaMP1b-EGFP

    Plasmid

    #138328

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1b
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. BIC-Gag-CAS9

    Plasmid

    #119942

    Purpose

    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

    Depositor

    Théophile Ohlmann, Emiliano Ricci, Philippe Mangeot

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG FMLV fused to spCAS9
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCR4-24XPP7SL

    Plasmid

    #31864

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    24xPP7 Stem Loop Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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