We narrowed to 15,661 results for: genome

Showing: 5281 - 5320 of 15661 results

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p15a_AcCas12n

Plasmid

#203806

Purpose

p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12n

Use

CRISPR

Promoter

rpsl

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCB578

Plasmid

#141095

Purpose

E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Inserts

Cas9 and tracrRNA
repeat-spacer-repeat array

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLdCN

Plasmid

#84290

Purpose

Express gRNA and Cas9 in Leishmania with Neomycin resistance

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

gRNA and Cas9

Use

CRISPR; Leishmania

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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NLS-SpCas9mSA-His

Plasmid

#182951

Purpose

Express SpCas9mSA in bacteria

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

NLS-SpCas9mSA-His

Use

CRISPR

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pnCasPA-BEC

Plasmid

#113349

Purpose

A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_B2515

Plasmid

#91072

Purpose

Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

AtU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0112

Plasmid

#177044

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase (CrGES) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0818

Plasmid

#177020

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geranyl pyrophosphate synthase; geranyl diphosphate synthase

Use

Synthetic Biology

Mutation

transit peptide removed

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Article

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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All_in_one_CRISPR/Cas9_LacZ

Plasmid

#74293

Purpose

"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

Depositor

Lynne Postovit

Article

Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

Inserts

LacZ-alpha
Cas9
mCherry

Use

CRISPR

Tags

HA

Expression

Bacterial and Mammalian

Promoter

Lac promoter, SV40, and T7 promoter

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB) (PX460)

Plasmid

#48873

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV- PRDX2

Plasmid

#31338

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PRDX2 (PRDX2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pNP2922 [pNP2321 - Cloning backbone, bRNA Landing Pad, with Ef1a-ISCro4 Recombinase]

Plasmid

#247255

Purpose

Cloning backbone for cloning bridge RNAs for ISCro4

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CRBN-P2A-Hygro

Plasmid

#124303

Purpose

Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CRBN (CRBN Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

Plasmid

#203842

Purpose

Flp-dependent Cre and EGFP expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has Service

AAV8

Insert

Cre

Use

AAV

Tags

EGFP-2A

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pNP3620 [pNP2916 - ISCro4 bRNA (TBL2, WT + DBL1, WT)]

Plasmid

#247267

Purpose

Express ISCro4 bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2916 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSIBR-Int4-Cas9-NT

Plasmid

#235166

Purpose

Expresses the SIBR-Int4-Cas9 system and a NT sgRNA

Depositor

Constantinos Patinios

Article

Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

Insert

SIBR-Int4-Cas9

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pDL17

Plasmid

#106483

Purpose

pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
exo
lacI
rhaR
rhaS

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2797

Plasmid

#63639

Purpose

pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2875

Plasmid

#63640

Purpose

pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2989

Plasmid

#63636

Purpose

pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Expression

Worm

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MiniCoopR mitfa:EGFP

Plasmid

#118850

Purpose

Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

mitfa: EGFP (mitfa Synthetic, Zebrafish)

Use

Zebrafish expression, tol6

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Promoter

Ptet

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 15,661 results for: genome