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8,637 results

Showing: 6851 - 6900 of 8637 results

  2. pT2/BH

    Plasmid
    #26556
    Depositor
    Perry Hackett
    Insert
    pT2/BH
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    26556-plasmid-map-sequence-id-203999

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  6. pUHD Cdk1 WT HA

    Plasmid
    #27652
    Depositor
    Greg Enders
    Insert
    Cdk1 (CDK1 Human)
    Use
    Tags
    HA (YDVPDYA)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. pKHR8

    Plasmid
    #74625
    Purpose
    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Inserts
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    Use
    Cre/Lox; Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-crystallin and cmlc2
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74625-plasmid-map-sequence-id-200444

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  15. pAAV-Syn-SomaGCaMP7

    Plasmid
    #158759
    Purpose
    Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158759-plasmid-map-sequence-id-310665

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  18. mCherry-Farnesyl-5

    Plasmid
    #55045
    Purpose
    Localization: Plasma Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55045-plasmid-map-sequence-id-314088

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  20. pLionII

    Plasmid
    #1730
    Purpose
    This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system
    Depositor
    Garry Nolan
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1730-plasmid-map-sequence-id-228221

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  21. pSBbi-RH

    Plasmid
    #60516
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60516-plasmid-map-sequence-id-97530

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  31. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Use
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  37. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  39. pN1 Lck-GCaMP5G

    Plasmid
    #34924
    Purpose
    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane
    Depositor
    Baljit Khakh
    Article
    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.
    Insert
    Lck-GCaMP5G
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Plasmid contains N-terminal 26 amino acid membran…
    Promoter
    CMV
    Available since
    Feb. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    34924-plasmid-map-sequence-id-42429

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  49. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

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Showing: 6851 - 6900 of 8637 results