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20+ requests

19,331 results

Showing: 521 - 560 of 19331 results

  10. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    David Bartel
    Article
    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12178-plasmid-map-sequence-id-181181

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  16. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Tags
    Expression
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

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  20. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid
    #113950
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from HLA class I …
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113950-plasmid-map-sequence-id-224188

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  22. pVITRO1-102.1F10-IgE/λ

    Plasmid
    #50365
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50365-plasmid-map-sequence-id-367944

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  23. pSLTS

    Plasmid
    #59386
    Purpose
    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Type
    Empty backbone
    Use
    Genome engineering
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59386-plasmid-map-sequence-id-380750

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  26. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101893-plasmid-map-sequence-id-211159

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  27. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαs-LgB113 (GNAS Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  28. GLuc-PM

    Plasmid
    #164783
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    Use
    Tags
    GLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164783-plasmid-map-sequence-id-326675

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  32. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

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  34. pIGneo

    Plasmid
    #52116
    Purpose
    a bicistronic IRES-GFP containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Tags
    IRES-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52116-plasmid-map-sequence-id-206319

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  40. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
    Use
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

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Showing: 521 - 560 of 19331 results