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cytoplasmic EKAR (Cerulean-Venus)

Plasmid

#18679

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nup88

Plasmid

#64283

Purpose

GFP-tagged mammalian expression of full-length Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
p4xACO-Luc

Plasmid

#16533

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

4xACO

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Km

Plasmid

#46813

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and PGK1

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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mEos3.2-Lifeact-7

Plasmid

#54696

Purpose

Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mEos3.2-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-link-yomCherry-Kan

Plasmid

#44903

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS082

Plasmid

#90516

Purpose

sgRNA entry vector

Depositor

Tom Ellis

Insert

Yeast sgRNA expression vector

Use

CRISPR; Yeast gap repair

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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CF-Ins54p

Plasmid

#20155

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (INP54 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Stop codon at position 331.

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGs

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-GFPd2

Plasmid

#14760

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFPd2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

David Root, John Doench

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI-IRES-Bla-Ak-ACE2-TMPRSS2

Plasmid

#154983

Purpose

Lentiviral vector expressing Human ACE2 and TMPRSS2

Depositor

Sonja Best

Insert

ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-NTRK2

Plasmid

#23883

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK2 (NTRK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-Rheb

Plasmid

#19996

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 (RHEB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Use

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
multi-shRNA vector

Plasmid

#12391

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP3SA11

Plasmid

#89170

Purpose

Express Rotavirus SA11 NSP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Lb

Plasmid

#86197

Purpose

LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

gRNA cloning site

Use

CRISPR

Tags

Expression

Mutation

Promoter

Maize ubiquitin 1

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-roxSTOProx-ZsGreen

Plasmid

#51274

Purpose

Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

roxSTOProx-ZsGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5 PPAR alpha

Plasmid

#22751

Depositor

Bruce Spiegelman

Insert

PPARalpha (Ppara Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-6XHis-FLKSRP

Plasmid

#23001

Depositor

Douglas Black

Article

Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

Insert

KSRP (KHSRP Human)

Use

Tags

EGFP and His

Expression

Mammalian

Mutation

valine inserted at aa#2

Promoter

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCP FLAG B55 alpha

Plasmid

#13804

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha Flag (PPP2R2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
C1(1-29)-miniTurbo-V5_pLX304

Plasmid

#107176

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Lentiviral

Tags

Expression

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-tTA

Plasmid

#99118

Purpose

An AAV genome that ubiquitously expresses tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV2

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPZpGAL4DBDUw

Plasmid

#26233

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

Zip-GAL4DBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMVtight Puro DEST (w768-1)

Plasmid

#26430

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNCX chick v-src

Plasmid

#14578

Depositor

Joan Brugge

Insert

v-Src (SRC Chicken)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
mTFP1-C1

Plasmid

#54613

Purpose

Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Promoter

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP1SA11

Plasmid

#89168

Purpose

Express Rotavirus SA11 NSP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-GFP

Plasmid

#58867

Purpose

AAV expression of GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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