Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,521 results

Showing: 5801 - 5840 of 179521 results

  2. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SHP2 (PTPN11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  7. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    178885-plasmid-map-sequence-id-354971

    Enlarge

  8. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid
    #171379
    Purpose
    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized Cas13X.1
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171379-plasmid-map-sequence-id-337344

    Enlarge

  10. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Publication
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Promoter
    T7, tetPA
    Available since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    161875-plasmid-map-sequence-id-316881

    Enlarge

  12. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Publication
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    155297-plasmid-map-sequence-id-307858

    Enlarge

  13. CGBE1 (pRZ3885)

    Plasmid
    #140252
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, D10A in Cas9
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140252-plasmid-map-sequence-id-291762

    Enlarge

  14. GFP-Dcp1a

    Plasmid
    #153972
    Purpose
    Fluorescent protein fused to mRNA decapping enzyme 1a
    Depositor
    Gia Voeltz
    Publication
    Lee et al Science. 2020 Jan 31;367(6477). pii: 367
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRNA decapping enzyme 1a (DCP1A Human)
    Tags
    pAcGFP-C1
    Expression
    Mammalian
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153972-plasmid-map-sequence-id-306138

    Enlarge

  17. pLKO.1 mCherry

    Plasmid
    #128073
    Purpose
    puro exchanged to mCherry
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128073-plasmid-map-sequence-id-255273

    Enlarge

  23. pB-CAGGS-dCas9-KRAB

    Plasmid
    #110822
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110822-plasmid-map-sequence-id-265907

    Enlarge

  30. pMaRSC

    Plasmid
    #89189
    Purpose
    The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.
    Depositor
    David Tirrell
    Publication
    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flag-L274G-T2A-mCherry (Mars1 Mouse)
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89189-plasmid-map-sequence-id-175705

    Enlarge

  32. pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84239
    Purpose
    Expresses ABA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sp dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84239-plasmid-map-sequence-id-160811

    Enlarge

  33. pLV_Zika_NS5_Flag

    Plasmid
    #79639
    Purpose
    Third generation Self-inactivating lentivector expressing NS5 from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS5
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79639-plasmid-map-sequence-id-148807

    Enlarge

  38. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

    Enlarge

  40. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72248-plasmid-map-sequence-id-131868

    Enlarge

Showing: 5801 - 5840 of 179521 results