162,536 results

1. pDONR221_SLC6A15

   Plasmid

   #131877

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A15 (SLC6A15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pX330A_dCas9-1x4

   Plasmid

   #63598

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD_dGFPmut3-AAV

   Plasmid

   #187379

   Purpose

   arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

   Depositor

   Roi Avraham

   Insert

   dGFPmut3_AAV
   

   Use

   Tags

   AAV

   Expression

   Bacterial

   Mutation

   C-terminal AAV tail

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH-AFID-3

   Plasmid

   #167361

   Purpose

   For inducing predictable deletions in rice plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MSCV-N E4orf1

   Plasmid

   #38063

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   E4 orf1
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC2HB

   Plasmid

   #63734

   Purpose

   bait plasmid for an adapted two-hybrid system for C. albicans

   Depositor

   Patrick Van Dijck

   Article

   Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

   Type

   Empty backbone

   Use

   C. albicans bait plasmid

   Tags

   HA, LexA, and NLS

   Expression

   Mutation

   Promoter

   Met3

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST2T18ms

   Plasmid

   #72647

   Purpose

   Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pJK-caCas9-NatMX-Neut5L-Leu2 drive

   Plasmid

   #89578

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Leu2 gene drive
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDe-CAS9

   Plasmid

   #61433

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   PcUBI4-2

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TH

    Plasmid

    #39079

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TH (TH Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pC2HP

    Plasmid

    #63735

    Purpose

    prey plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans prey plasmid

    Tags

    FLAG, NLS, and VP domain

    Expression

    Mutation

    Promoter

    Met3

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71294

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    GNSTM glycosylation motif, HA, Lamp2 signal pepti…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGFP-Cytochrome C

    Plasmid

    #41181

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C (CYCS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. scAAV-hIBA1a-GFP

    Plasmid

    #214146

    Purpose

    Self-complementary AAV vector packaging GFP under the human Iba1 promoter

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD2:35S-TMV-Rep-MP

    Plasmid

    #106217

    Purpose

    Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

    Depositor

    Tomáš Moravec

    Insert

    Infectious clone of Tobacco mosaic virus, 5' part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_PKIA_WT

    Plasmid

    #82153

    Purpose

    Gateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PKIA (PKIA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSG5-HA-p300

    Plasmid

    #89094

    Purpose

    mammalian expression of full-length human p300 in pSG5

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5_mEGFP-CRTC1-MAML2

    Plasmid

    #237638

    Purpose

    For overexpression of mEGFP-CRTC1-MAML2

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    CRTC1-MAML2 (CRTC1, MAML2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMPMA3dPlac-PpipB-GFPLVA

    Plasmid

    #23344

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AY22_pgRNA.R5.1

    Plasmid

    #100294

    Purpose

    CCR5-targeting gRNA expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 RNA Pol III promoter human snRNA

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGCS-C6(eGFP)

    Plasmid

    #85729

    Purpose

    Gateway destination vector

    Depositor

    Hai-Ning Du

    Article

    Wang et al Acta Biochim Biophys Sin (Shanghai). 201

    Type

    Empty backbone

    Use

    Mammalian/avian/xenopus/zebrafish

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSNAP-CLC-SNAP

    Plasmid

    #38012

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    SNAP26m

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mEmerald-Sec23A

    Plasmid

    #166893

    Purpose

    mammalian expression of Sec23A tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    SEC23A (SEC23A Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

    Plasmid

    #170712

    Purpose

    dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Inserts

    mNeonGreen
    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TNNT2 and aMHC

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRZ-CAS9-mCherry

    Plasmid

    #80974

    Purpose

    Cas9, mCherry expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET19b-rDAD2 cPGFP(G164)

    Plasmid

    #165994

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(G164)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pWPI-N3ICD

    Plasmid

    #185524

    Purpose

    Constitutive overexpression of human NOTCH3 intracellular domain

    Depositor

    Eric Shusta, Sean Palecek

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH3 intracellular domain (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. iLID-GFP-GCN4-ppKin14

    Plasmid

    #240420

    Purpose

    -end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    ppKin14Vib
    

    Use

    Tags

    GCN4, GFP, and iLID

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HA-KIF5A-FRB

    Plasmid

    #240425

    Purpose

    +end oriented kinesin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Vim-mCh-FKBP

    Plasmid

    #240423

    Purpose

    Tagged vimentin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. KIF5A-mVenus-iLID

    Plasmid

    #240422

    Purpose

    +end oriented kinesin for optogenetic pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    iLID and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Vim-mCh-SspB

    Plasmid

    #240421

    Purpose

    Tagged vimentin for optogenetic vimentin pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    SspB and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FRB-BFP-GCN4-ppKin14

    Plasmid

    #240426

    Purpose

    -end oriented kinesin for rapalog induced pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    ppKin14Vib
    

    Use

    Tags

    FRB, GCN4, and tagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge