159,258 results

1. tubP-FRT>GAL80-6-FRT>

   Plasmid

   #63173

   Purpose

   ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "Flp-Out" GAL80
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR4-HaloTag (w876-1)

   Plasmid

   #29644

   Depositor

   Eric Campeau

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-p18G2A-HA

   Plasmid

   #42327

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p18 G2A (LAMTOR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G2A

   Promoter

   CMV, SP6

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCMV(MinDis).iGluSnFR

   Plasmid

   #41732

   Purpose

   Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   Myc, PDGFR transmembrane helix (aa 513-561), and …

   Expression

   Mammalian

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   CMV

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWP78-PVCpnf1-16

   Plasmid

   #198271

   Purpose

   Unmodified pPVC; contains WT PVC structural/accessory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf1-16
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-hACE2

   Plasmid

   #145033

   Purpose

   Express human ACE2 with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   human ACE2 (ACE2 Human)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. mCherry-pCAG-donor(R2Tg)-GFP(intron)

   Plasmid

   #223248

   Purpose

   Mammalian expression of the R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACU2_CD4-mIFP T2A HO1

   Plasmid

   #72441

   Purpose

   Expressing UAS-CD4-mIFP T2A HO1 in drosophila

   Depositor

   Xiaokun Shu

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CD4-mIFP T2A HO1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid

    #192552

    Purpose

    Expresses nuclear localized tdTomato under CAG promoter

    Depositor

    Guoping Feng

    Has Service

    AAV PHP.eB and AAV9

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    deleted E273 and G274 compared to FPbase ID: PGG5S

    Promoter

    CAG

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-TK-5UTR-BsmBI-Luciferase

    Plasmid

    #114670

    Purpose

    For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

    Depositor

    Lynne Postovit

    Article

    Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

    Insert

    LacZ
    

    Use

    Luciferase

    Tags

    for cloning 5' UTR-Luciferase fusions

    Expression

    Mammalian

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAY5 (mAID-EGFP-NLS piggyBac)

    Plasmid

    #140532

    Purpose

    mAID-EGFP-NLS piggyBac

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eGFP STAT1 WT

    Plasmid

    #12301

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Jun 26. ():.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRsetB-PercevalHR

    Plasmid

    #49081

    Purpose

    Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Syn-CD4-sTb(C)-M13-GFP

    Plasmid

    #219784

    Purpose

    sTb(C) half of CaST for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTb(C)-M13-GFP
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIS1-Eef25UTR-TOPmut-renilla

    Plasmid

    #38236

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The first nucleotides of the 5' UTR were cha…

    Promoter

    Endogenous Eef2

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

20. GFP KNL1 wt hardened

    Plasmid

    #45223

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    GFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)
    

    Use

    Tags

    TEV S15

    Expression

    Mutation

    E172G

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pCAGGS Donor mClover-LMNA

    Plasmid

    #98970

    Purpose

    Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST47-SARA

    Plasmid

    #67473

    Purpose

    Mammalian expression of hSara with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCDNA5/FRT-ChemoB-ATP

    Plasmid

    #193819

    Purpose

    CMV overexpression of ChemoB-ATP in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mEmerald-ALK5-N-13

    Plasmid

    #62751

    Purpose

    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    ALK5 (TGFBR1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pBluescript-mouse cyclin D2

    Plasmid

    #86791

    Purpose

    bacterial expression of mouse cyclin D2

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D2 (Ccnd2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCMV4-3 HA/IkB-alpha(SS32,36AA)

    Plasmid

    #24143

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    IkB alpha (NFKBIA Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S32A S36A

    Promoter

    Available since

    March 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV GFP

    Plasmid

    #49055

    Purpose

    adeno-associated virus (AAV) encoding a green fluorescent protein

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tdTomato-Keratin-17

    Plasmid

    #58106

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pENN226_CRISPRcharm

    Plasmid

    #220834

    Purpose

    CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.4_StrepII_TEV_PP2Ac

    Plasmid

    #217477

    Purpose

    Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    PP2Ac
    

    Use

    Tags

    StrepII, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-Rac1-2G

    Plasmid

    #66111

    Purpose

    Second generation Rac1 FRET biosensor for lentivirus production

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

    Plasmid

    #170048

    Purpose

    Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    mWasabi dN
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLQ6332 - ACTB CRISPRa CLIP Donor

    Plasmid

    #210015

    Purpose

    CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    dLbCpf1-miniVPR-T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTP288

    Plasmid

    #113460

    Purpose

    human Flag tagged Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pQF2WB

    Plasmid

    #61312

    Purpose

    Enhancer trap version of QF2, analogous to pGAWB

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHAGE-MET

    Plasmid

    #116762

    Purpose

    Lentiviral expression of MET

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMVHA SUZ12

    Plasmid

    #24232

    Purpose

    Mammalian expression of human SUZ12 with HA tag

    Depositor

    Kristian Helin

    Article

    Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSTAR-mOrange2-sfGFP

    Plasmid

    #115693

    Purpose

    Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

    Depositor

    David Suter

    Article

    Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

    Insert

    SA-mOrange2-sfGFP-SD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Use

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. VPS13D^EGFP

    Plasmid

    #174109

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    With an internal EGFP tag in aminoacid position 1…

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pDNA2.0 6H-TEV-KRAS

    Plasmid

    #111849

    Purpose

    Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pPB-cT3G-cERP2-YBX1

    Plasmid

    #192904

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    YBX1 (YBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEVOL-pylT-AznLRS

    Plasmid

    #172482

    Purpose

    For E.Coli expression, 306L309L348I384F411W

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

    Insert

    AznLRS and pylTcua
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLenti-X1-Neo-HA-UFL1

    Plasmid

    #139860

    Purpose

    Lentiviral expression of HA-tagged human UFL1

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFL1 (UFL1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3-HA-CUL1 Y42A/M43A

    Plasmid

    #19940

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 Y42A/M43A (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    CUL1 with a SKP1 binding mutant

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only