Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

169,656 results

Showing: 7601 - 7650 of 169656 results

  11. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available Since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

    Enlarge

  16. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

    Enlarge

  20. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

    Enlarge

  24. plix403-V5-N-mEGFP

    Plasmid
    #223957
    Purpose
    Gateway cloning of genes of interest with an N-terminal V5-mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-mEGFP
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223957-plasmid-map-sequence-id-450838

    Enlarge

  28. TFORF1713

    Plasmid
    #142832
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LDB2 (LDB2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142832-plasmid-map-sequence-id-396018

    Enlarge

  35. pJK369

    Plasmid
    #73594
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    FLAG tag
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73594-plasmid-map-sequence-id-136122

    Enlarge

  36. AAV-CAG-jGCaMP8s-WPRE

    Plasmid
    #179256
    Purpose
    AAV-mediated expression of GCaMP8s under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8s
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179256-plasmid-map-sequence-id-354036

    Enlarge

  38. pCMV-MA-VN

    Plasmid
    #123283
    Purpose
    Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    Matrix
    Tags
    VN: N-terminus of split Venus (a.a. V1–A154) I152…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123283-plasmid-map-sequence-id-239665

    Enlarge

  39. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Available Since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98306-plasmid-map-sequence-id-181809

    Enlarge

  40. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Promoter
    TRE3GV
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

    Enlarge

  46. STARR-seq luciferase validation vector_SCP1_CMV

    Plasmid
    #99314
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99314-plasmid-map-sequence-id-190943

    Enlarge

Showing: 7601 - 7650 of 169656 results