9,049 results

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pKA-207I10

Plasmid

#79845

Purpose

Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSS9

Plasmid

#71655

Purpose

Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

gfpuv

Use

Synthetic Biology

Promoter

T7A1

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETA-PAGFP

Plasmid

#11911

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Insert

pRSETA-PAGFP

Expression

Bacterial

Mutation

The PAGFP has four mutations (L64F/T65S/V163A/T20…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP15

Plasmid

#141268

Purpose

Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLew100::NLS-ISceI-HA

Plasmid

#21299

Depositor

Nina Papavasiliou

Article

Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

Insert

I-SceI (SCEI )

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-Renilla cDNA

Plasmid

#29783

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

Renilla

Use

Lentiviral

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del1(T790M)

Plasmid

#32072

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete E746-A750, T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Del 2)

Plasmid

#16602

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 2) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-2 (KpnI->PvuII). The KpnI site is at -1194…

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pCMV4-NIK-HA

Plasmid

#27554

Depositor

Shao-Cong Sun

Article

Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

Insert

NIK (MAP3K14 Human)

Tags

HA

Expression

Mammalian

Mutation

NIK starts at amino acid 3; C52R and R139H mutati…

Promoter

CMV

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-RET

Plasmid

#23906

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RET (RET Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-14-3-3 eta GST

Plasmid

#13277

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 eta (YWHAH Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pEYFP SUMO-1

Plasmid

#13380

Depositor

Mary Dasso

Article

Ayaydin et al Mol Biol Cell. 2004 Dec . 15(12):5208-18

Insert

SUMO-1 (SUMO1 Human)

Tags

EYFP

Expression

Mammalian

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pTrcAgBIS (CO)

Plasmid

#35153

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pTrc

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG RIG-I MIII

Plasmid

#52877

Purpose

Expresses RIG-I with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

DDX58 (RIGI Human)

Tags

FLAG

Expression

Mammalian

Mutation

T409A, S411A

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-ERG

Plasmid

#51301

Purpose

Expression of ERG after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-rab9 DN

Plasmid

#12664

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pC017 - LwCas13a guide expression backbone with tRNAval promoter

Plasmid

#91907

Purpose

Backbone for cloning LwCas13a guides under tRNAval promoter

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a Guide site

Expression

Mammalian

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-YEE-BE3

Plasmid

#85177

Purpose

Expresses YEE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-UtrCH

Plasmid

#26739

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

monomeric RFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS-NICD

Plasmid

#26891

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD (Notch1 Mouse)

Tags

FLAG and b-globin polyA

Expression

Mammalian

Mutation

E2299K

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
Nampt-PET28a

Plasmid

#25630

Depositor

Cynthia Wolberger

Article

Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

Insert

Nampt (Nampt Mouse)

Tags

His6

Expression

Bacterial

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
hTLR3 flag

Plasmid

#13084

Depositor

Ruslan Medzhitov

Insert

TLR3 (TLR3 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-17 have been replaced by mammalian …

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pFLAG/HA-DGCR8

Plasmid

#10921

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

DGCR8 (DGCR8 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
E[beta]P

Plasmid

#24313

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBF3038

Plasmid

#26850

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Otx2

Plasmid

#35001

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Homeobox protein OTX2 (Otx2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-c-Myc-IP

Plasmid

#15921

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
IRE1 alpha KA-pcDNA3.EGFP

Plasmid

#13010

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Expression

Mammalian

Mutation

R4L, K599A

Promoter

CMV

Available Since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlagNICD4

Plasmid

#20186

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 4 Intracellular Domain (Notch4 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 4 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
pCIP-caTfeb

Plasmid

#79013

Purpose

For mammalian expression of a shortened, constitutively active version of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (TFEB Human)

Expression

Mammalian

Mutation

Removed first ~400 bp from 5' end (N-terminu…

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
901 pLNCX myr HA Akt1

Plasmid

#9005

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-YTB

Plasmid

#26721

Depositor

Edward Callaway

Article

Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

Insert

venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Chicken, Rabies virus (strain SAD B19))

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
T/Brachyury-eGFP Rex Neo

Plasmid

#21222

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFLAG-dCas9/pMXs-neo

Plasmid

#51260

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA cyclin D1 HA

Plasmid

#11181

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-USP42

Plasmid

#22603

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP42 (USP42 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
hMSH2 Clone E3 (full-length)

Plasmid

#16453

Depositor

Bert Vogelstein

Article

Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

Insert

MSH2 (MSH2 Human)

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 NSP10

Plasmid

#141264

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 3, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
piRFP682-N1

Plasmid

#45459

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Expression

Mammalian

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn1-mRuby2

Plasmid

#99126

Purpose

An AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-KDR

Plasmid

#23925

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KDR (KDR Human)

Use

Gateway donor vector

Mutation

V297I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19/human IL-8

Plasmid

#17610

Depositor

Joost Oppenheim

Article

Matsushima et al J Exp Med. 1988 Jun 1. 167(6):1883-93.

Insert

IL8 (CXCL8 Human)

Expression

Bacterial

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-TRBP

Plasmid

#15666

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

TRBP (TARBP2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
Flag pLJM1 RagB 54L

Plasmid

#19314

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T54L

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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9,049 results